Investigation of endothelin-1 type A receptor gene polymorphism (−231 G &gt; A) in preeclampsia susceptibility

Lisi,; Paternoster, D.M.; Stecca, Anna; Miccichè, Flavia; Fantinato, S.; Leon, A. S.; Damante, Giuseppe; Fabbro, Dora; Clementi, Maurizio

doi:10.1080/14767050601127797

Cited by 7 publications

(5 citation statements)

References 27 publications

(30 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microarray analysis indicated a number of genes that were up- or down-regulated by the miR-518b mimic. Among the 124 target genes down-regulated more than 2-fold (Supplementary Table S1), two genes (tyrosine hydroxylase: TH and hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1: HSD3B1 ) were previously demonstrated to be involved in the pathogenesis of preeclampsia202122, and five genes (endothelin receptor type A: EDNRA , advanced glycosylation end product-specific receptor: AGER , wingless-type MMTV integration site family member 2: WNT2 , complement component 9: C9 , and transient receptor potential cation channel, subfamily M, member 2: TRPM2 ) play roles in preeclampsia with foetal growth restriction23242526272829303132 (Table 1). Likewise, among the 112 target genes up-regulated over 2-fold by the miR-518b mimic (Supplementary Table S2), four genes [hemopexin: HPX , serpin peptidase inhibitor, clade B (ovalbumin), member 2: SERPINB2 , lipoprotein, Lp(a): LPA , and tumour necrosis factor superfamily, member 10: TNFSF10 ] show involvement in preeclampsia35363738, and two genes (CD69 molecule: CD69 and stanniocalcin 1: STC1 ) are involved in preeclampsia with foetal growth restriction333439 (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…miR-518b seems to control multiple target genes located on various chromosomes. Interestingly, some miR-518b target genes were previously demonstrated to associate with preeclampsia (e.g., TH and HSD3B1 for down-regulated genes, and HPX, SERPINB2, LPA and TNFSF10 for up-regulated genes)20212235363738 and with preeclampsia with foetal growth restriction (e.g., EDNRA, AGER, WNT2, C9 , and TRPM2 for down-regulated genes, and CD69 and STC1 for up-regulated genes)23242526272829303132333439. However, further experiments are needed to demonstrate a causal relationship between the expression of pregnancy-associated miRNAs and pregnancy-related disorders.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Feasibility of placenta-derived mesenchymal stem cells as a tool for studying pregnancy-related disorders

Fuchi

Miura

Doi

et al. 2017

Sci Rep

View full text Add to dashboard Cite

The cellular and molecular mechanisms responsible for pregnancy-related disorders remain unclear. We investigated the feasibility of using placenta-derived mesenchymal stem cells (MSCs) as a tool to study such pregnancy-related disorders. We isolated and expanded adequate numbers of cells with characteristic features of MSCs from the chorionic plate (CP-MSCs), chorionic villi (CV-MSCs), and decidua basalis (DB-MSCs) of human term placental tissues. All placenta-derived MSCs expressed pregnancy-associated C14MC microRNA (miRNA) (miR-323-3p). Interestingly, the placenta-specific C19MC miRNAs (miR-518b and miR517a) were clearly expressed in CP-MSCs and CV-MSCs of foetal origin, but were barely expressed in DB-MSCs of maternal origin. Furthermore, expression levels of placenta-specific C19MC miRNAs in CV-MSCs remained stable during the ex vivo expansion process and across different pregnancy phases (first trimester versus third trimester). High-efficiency siRNA transfection was confirmed in twice-passaged CV-MSCs with little toxicity, and microarray analysis was used to screen for miR-518b target genes. Placenta-derived MSCs, especially CV-MSCs, are a potential tool for investigating the role of placental miRNAs in pregnancy-related disorders.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Feasibility of placenta-derived mesenchymal stem cells as a tool for studying pregnancy-related disorders

Fuchi

Miura

Doi

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Wang et al showed a significant association between the TNF-α-308G/A polymorphism and parity (Wang et al, 2018). Similarly, no significant relation in a study by Lisi et al was found between preeclampsia risk factors, such as systolic and diastolic blood pressure, proteinuria and endothelin-1 type-A receptor gene polymorphism (7231 G4A) [40]. Although the current report revealed the genetic association between rs2069740(T/A) and rs34255686(C/A) polymorphisms in the IL-13 gene and the risk of preeclampsia, a more significant sample size is required to validate the observations of the present study.…”

Section: Discussionmentioning

confidence: 87%

Preeclampsia Susceptibility Assessment Based on Deep Learning Modeling and Single Nucleotide Polymorphism Analysis

et al. 2023

View full text Add to dashboard Cite

The early diagnosis of preeclampsia, a key outlook in improving pregnancy outcomes, still remains elusive. The present study aimed to examine the interleukin-13 and interleukin-4 pathway potential in the early detection of preeclampsia as well as the relationship between interleukin-13 rs2069740(T/A) and rs34255686(C/A) polymorphisms and preeclampsia risk to present a combined model. This study utilized raw data from the GSE149440 microarray dataset, and an expression matrix was constructed using the RMA method and affy package. The genes related to the interleukin-13 and interleukin-4 pathway were extracted from the GSEA, and their expression levels were applied to design multilayer perceptron and PPI graph convolutional neural network models. Moreover, genotyping for the rs2069740(T/A) and rs34255686(C/A) polymorphisms of the interleukin-13 gene were tested using the amplification refractory mutation system PCR method. The outcomes revealed that the expression levels of interleukin-4 and interleukin-13 pathway genes could significantly differentiate early preeclampsia from normal pregnancy. Moreover, the present study’s data suggested significant differences in the genotype distribution, the allelic frequencies and some of the risk markers of the study, in the position of rs34255686 and rs2069740 polymorphisms between the case and control groups. A combined test of two single nucleotide polymorphisms and an expression-based deep learning model could be designed for future preeclampsia diagnostic purposes.

show abstract

“…It is suggested that the fetus itself is also synthesizing the hormone, as a result of hemodynamic and metabolic changes occurring during uterine contractions in childbirth. It is likely that an increased concentration of EDN1in umbilical blood plasma during childbirth leads to the contraction of umbilical vessels after delivery, which may be one of the mechanisms that prepare the fetus for taking the first breath 25 . Plasma endothelin-1 concentrations were similar in preterm and term infants studied by Kuo Cy 26 and Stefanov G et al .…”

Section: Discussionmentioning

confidence: 99%

Role of endothelial nitric oxide synthase and endothelin-1 polymorphism genes with the pathogenesis of intraventricular hemorrhage in preterm infants

Szpecht

Gadzinowski

Seremak‐Mrozikiewicz

et al. 2017

Sci Rep

View full text Add to dashboard Cite

In the pathogenesis of neonatal intraventricular hemorrhage (IVH) in preterm infants, an important role is played by changes in venous and arterial cerebral flows. It has been shown that the ability of autoregulation of cerebral flows in response to variations in arterial blood pressure in preterm infants is impaired. This impaired autoregulation causes an increased risk of germinal matrix rupture and IVH occurrence. We examined three polymorphisms of genes, related to regulation of blood flow, for an association with IVH in 100 preterm infants born from singleton pregnancy, before 32 + 0 weeks of gestation, exposed to antenatal steroids therapy, and without congenital abnormalities. These polymorphisms include: eNOS (894G > T and −786T > C) and EDN1 (5665G > T ) gene. We found that infants with genotype GT eNOS 894G > T have 3.4-fold higher risk developing of IVH born before 28 + 6 weeks of gestation. Our investigation did not confirm any significant prevalence for IVH development according to eNOS −786T > C genes polymorphism. Our novel investigations in EDN1 5665G > T polymorphism did not show any link between alleles or genotypes and IVH. Future investigations of polymorphisms in blood-flow associated genes may provide valuable insight into the pathogenetic mechanisms underlying the development of IVH.

show abstract

Investigation of endothelin-1 type A receptor gene polymorphism (−231 G > A) in preeclampsia susceptibility

Abstract: No association between the -231 G > A polymorphism in the EDNRA gene and preeclampsia as well as any correlation with the main clinical features of the disorder were found, thus excluding a role for this polymorphism in susceptibility to preeclampsia.

Cited by 7 publications

References 27 publications

Feasibility of placenta-derived mesenchymal stem cells as a tool for studying pregnancy-related disorders

Feasibility of placenta-derived mesenchymal stem cells as a tool for studying pregnancy-related disorders

Preeclampsia Susceptibility Assessment Based on Deep Learning Modeling and Single Nucleotide Polymorphism Analysis

Role of endothelial nitric oxide synthase and endothelin-1 polymorphism genes with the pathogenesis of intraventricular hemorrhage in preterm infants

Contact Info

Product

Resources

About