Investigation of Efficacy Enhancing and Toxicity Reducing Mechanism of Combination of Aconiti Lateralis Radix Praeparata and Paeoniae Radix Alba in Adjuvant-Induced Arthritis Rats by Metabolomics

Xie, Yunfei; Feng, Wuwen; Liu, Meichen; Xie, Jun; Yu, Long-Chuan; Gong, Xiaohong; Li, Yunxia; Peng, Cheng

doi:10.1155/2019/9864841

Cited by 19 publications

(21 citation statements)

References 60 publications

(58 reference statements)

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“…Meanwhile, FLS is considered to be the main cellular source of COX-2 in synovial tissue and produce PGE2, which mediates the pain and in ammation in RA (Amin et al, 1999). Notably, in previous research, we have demonstrated that FLA signi cantly alleviated the symptoms of arthritis in AIA mice (Xie et al, 2019). This study found that FLA effectively inhibited the expression and synthesis of pro-in ammatory mediators in IL-1β-induced HFLS-RA.…”

Section: Discussionmentioning

confidence: 53%

Aconiti Lateralis Radix Praeparata Lipid-Soluble Alkaloids Alleviates IL-1β-Induced Inflammation of Human Fibroblast-Like Synoviocytes in Rheumatoid Arthritis by Inhibiting NF-κB and MAPKs Signaling Pathways and Inducing Apoptosis

Guo¹,

He²,

Hu³

et al. 2021

Preprint

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Background: Fuzi lipid-soluble alkaloids (FLA) is the main bioactive components extracted from the traditional Chinese medicine Aconiti Lateralis Radix Praeparata (“Fuzi” in Chinese), which has promising analgesic and anti-inflammatory effects. However, the effects and the underlying mechanisms of FLA on rheumatoid arthritis (RA) have not been studied. The present study aimed to explore the anti-arthritic effects of FLA and its underlying mechanisms. Methods: To standardize the FLA, UPLC-HR-MS was used for quantitative and qualitative analysis of the representative alkaloids. Cell viability was measured by MTT. The anti-inflammatory activity of FLA was examined by analyzing the expression levels of inflammatory mediators such as TNF-α, IL-6, MMP-1, MMP-3, PGE2, and COX-2 using ELISA and RT-PCR analysis. The Annexin V-FITC/PI double staining method was used to detect the apoptosis of HFLS-RA and analyzed by flow cytometry. Western blot analysis was used to analyze the expression of NF-κB, MAPKs and mitochondrial apoptosis pathway related proteins. Results: FLA had a significant inhibitory effect on the proliferation of HFLS-RA induced by IL-1β, which was accompanied by decreased expression levels of TNF-α, IL-6, MMP-1, MMP-3, COX-2 and PGE2. Remarkably, FLA inhibited the activation of NF-κB and MAPKs signaling pathways in IL-1β-induced HFLS-RA, as well as inducing HFLS-RA apoptosis through the mitochondrial apoptosis pathway. Conclusions: FLA inhibited the expression and synthesis of inflammatory mediators by inhibiting the activation of NF-κB and MAPKs signaling pathways in HFLS-RA, and induced apoptosis of HFLS-RA via the mitochondrial apoptosis pathway.

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Section: Discussionmentioning

confidence: 53%

Aconiti Lateralis Radix Praeparata Lipid-Soluble Alkaloids Alleviates IL-1β-Induced Inflammation of Human Fibroblast-Like Synoviocytes in Rheumatoid Arthritis by Inhibiting NF-κB and MAPKs Signaling Pathways and Inducing Apoptosis

Guo¹,

He²,

Hu³

et al. 2021

Preprint

Self Cite

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“…Currently, increasing attention has been paid to the role of natural herbal extracts with a probable therapeutic effect on the treatment of several diseases, including RA ( 37 , 38 ). For example, Astragalus membranaceus , Tripterygium wilfordii and Radix Paeoniae Alba have been proven to have certain therapeutic effects on RA ( 39 - 41 ). The therapeutic effect of traditional Chinese herbs on diseases mainly depends on a variety of active components.…”

Section: Discussionmentioning

confidence: 99%

Astragaloside regulates lncRNA LOC100912373 and the miR‑17‑5p/PDK1 axis to inhibit the proliferation of fibroblast‑like synoviocytes in rats with rheumatoid arthritis

Jiang

Chang

et al. 2021

Int J Mol Med

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Previous studies have confirmed that astragaloside (AST) exerts a positive effect on alleviating synovial and joint injury in rheumatoid arthritis (RA). However, the precise mechanisms through which AST acts in the treatment of RA remain unclear. Long non-coding RNA (lncRNA) LOC100912373 was identified as a key gene related to RA and has been proven to interact with miR-17-5p, in order to regulate the pyruvate dehydrogenase kinase 1 and protein kinase B axis (PDK1/AKT axis). The present study aimed to determine whether AST may treat RA through the interaction between lncRNA LOC100912373 and the miR-17-5p/PDK1 axis. MTT assays and flow cytometry were used to detect the proliferation and cell cycle progression of AST-treated fibroblast-like synoviocytes (FLSs). The expression of lncRNA LOC100912373 and miR-17-5p, as well as relative the mRNA expression of the PDK1 and AKT genes following AST intervention was detected by reverse transcription-quantitative PCR (RT-qPCR), immunofluorescence and western blot analysis. The results revealed that AST inhibited FLS proliferation, reduced lncRNA LOC100912373 expression levels, increased miR-17-5p expression levels, and decreased the PDK1 and p-AKT expression levels. Additionally, consecutive rescue experiments revealed that AST counteracted the effects of lncRNA LOC100912373 overexpression on FLS proliferation and cell cycle progression. On the whole, the present study demonstrates that AST inhibits FLS proliferation by regulating the expression of lncRNA LOC100912373 and the miR-17-5p/PDK1 axis.

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“…It is commonly used for blood deficiency, irregular menstruation, spontaneous sweating, night sweats, flank pain, abdominal pain, limb pain, headache, and dizziness. 6,7 Previous studies showed that PRA could be used for anti-inflammatory effects, 8 immune regulation, 9 treatment of depression 10,11 and anemia, 12 promotion of peripheral nerve regeneration, 13 and treatment of viral infections. 14 Modern studies of pharmacological mechanisms have found that the main active components of PRA include paeoniflorin, paeonol, paeonin, benzoylpaeoniflorin, albiflorin, oxypaeoniflorin, paeoniflorigenone, benzoic acid, β-sitosterol, and gal-lotannin.…”

Section: Pharmacological Studies On the Active Ingredients Of The Sixmentioning

confidence: 99%

<p>Research Progress on Classical Traditional Chinese Medicine Jieyu Pills in the Treatment of Depression</p>

Wang¹,

Miao²

2020

NDT

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Investigation of Efficacy Enhancing and Toxicity Reducing Mechanism of Combination of Aconiti Lateralis Radix Praeparata and Paeoniae Radix Alba in Adjuvant-Induced Arthritis Rats by Metabolomics

Cited by 19 publications

References 60 publications

Aconiti Lateralis Radix Praeparata Lipid-Soluble Alkaloids Alleviates IL-1β-Induced Inflammation of Human Fibroblast-Like Synoviocytes in Rheumatoid Arthritis by Inhibiting NF-κB and MAPKs Signaling Pathways and Inducing Apoptosis

Aconiti Lateralis Radix Praeparata Lipid-Soluble Alkaloids Alleviates IL-1β-Induced Inflammation of Human Fibroblast-Like Synoviocytes in Rheumatoid Arthritis by Inhibiting NF-κB and MAPKs Signaling Pathways and Inducing Apoptosis

Astragaloside regulates lncRNA LOC100912373 and the miR‑17‑5p/PDK1 axis to inhibit the proliferation of fibroblast‑like synoviocytes in rats with rheumatoid arthritis

<p>Research Progress on Classical Traditional Chinese Medicine Jieyu Pills in the Treatment of Depression</p>

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