Investigation of drought induced biochemical and gene expression changes in carrot cultivars

Junaid, Muhammad Daniyal; Gökçe, Zahide Neslihan Öztürk; Gökçe, Zahide Neslihan Öztürk

doi:10.1007/s11033-022-08050-4

Cited by 4 publications

(14 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Four CAT genes, protein transport SEC23, LEC14B homolog were enriched in the plum2 module. The cultivars Coral Orange, Tendersweet and Solar Yellow were tolerant to drought stress, which was supported by their higher transcript levels of CAT , SOD genes [ 76 ]. The drought tolerant cultivars have higher accumulated of CAT gene [ 77 ].…”

Section: Discussionmentioning

confidence: 99%

Physiological responses to drought stress of three pine species and comparative transcriptome analysis of Pinus yunnanensis var. pygmaea

Xiao,

Zhao,

Wang

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

Drought stress can significantly affect plant growth, development, and yield. Fewer comparative studies have been conducted between different species of pines, particularly involving Pinus yunnanensis var. pygmaea (P. pygmaea). In this study, the physiological indices, photosynthetic pigment and related antioxidant enzyme changes in needles from P. pygmaea, P. elliottii and P. massoniana under drought at 0, 7, 14, 21, 28 and 35 d, as well as 7 days after rehydration, were measured. The PacBio single-molecule real-time (SMRT) and Illumina RNA sequencing were used to uncover the gene expression differences in P. pygmaea under drought and rehydration conditions. The results showed that the total antioxidant capacity (TAOC) of P. pygmaea was significantly higher than P. massoniana and P. elliottii. TAOC showed a continuous increase trend across all species. Soluble sugar (SS), starch content and non-structural carbohydrate (NSC) of all three pines displayed a "W" pattern, declining initially, increasing, and then decreasing again. P. pygmaea exhibits stronger drought tolerance and greater recovery ability under prolonged drought conditions. Through the PacBio SMRT-seq, a total of 50,979 high-quality transcripts were generated, and 6,521 SSR and 5,561 long non-coding RNAs (LncRNAs) were identified. A total of 2310, 1849, 5271, 5947, 7710, and 6854 differentially expressed genes (DEGs) were identified compared to the control (Pp0D) in six pair-wise comparisons of treatment versus control. bHLH, NAC, ERF, MYB_related, C3H transcription factors (TFs) play an important role in drought tolerance of P. pygmaea. KEGG enrichment analysis and Gene set enrichment analysis (GSEA) analysis showed that P. pygmaea may respond to drought by enhancing metabolic processes such as ABA signaling pathway, alpha-linolenic acid. Weighted gene co-expression network analysis (WGCNA) revealed GST, CAT, LEC14B, SEC23 were associated with antioxidant enzyme activity and TAOC. This study provides a basis for further research on drought tolerance differences among coniferous species.

show abstract

Section: Discussionmentioning

confidence: 99%

Physiological responses to drought stress of three pine species and comparative transcriptome analysis of Pinus yunnanensis var. pygmaea

Xiao,

Zhao,

Wang

et al. 2024

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…Fluctuations in environmental conditions have remarkably influenced carrot growth and development (Huang et al 2015 ; Que et al 2019 ). Several studies described that water deficit is a major abiotic factor in carrot production and it negatively affects carrot yield and quality (Salter and Goode 1967 ; Groves and Bailey 1994 ; Herppich et al 2001 ; Öztürk Gökçe et al 2022 ; Junaid et al 2023a , b ). However, the influence of low soil moisture on carrot taproots is not clear.…”

Section: Drought Stressmentioning

confidence: 99%

“…However, the influence of low soil moisture on carrot taproots is not clear. Our recent study showed that water scarcity severely affects the chemical composition, dry matter content, and sugar content of susceptible carrot cultivars (Junaid et al 2023a , b ). Decrease in dry matter % under drought stress is also due to inhibition of biomolecules under stress and the inability of carrot cultivars in dry matter partitioning in different parts of their roots (Polania et al 2016 ).…”

Section: Drought Stressmentioning

confidence: 99%

“…Osmo-protectants control cell turgor, water gradient, and water uptake in the cytoplasmic region (Murti et al 2007 ). Different studies reported osmotic adjustment in carrots under abiotic stress (Kamińska et al 2022 ; Junaid et al 2023a ; Öztürk Gökçe et al 2022 ). However, studies are still needed to investigate mechanisms behind osmotic regulation in carrot plants under abiotic stress.…”

Section: Drought Stressmentioning

confidence: 99%

“…Root crops are highly sensitive to water deficit during root formation, hence water scarcity causes a negative influence on the root quality and yield of carrot plants (Junaid et al 2023a , b ). Drought stress threat is increasing at an alarming rate, every year occurrence of drought multiplies and results in severe yield and quality losses (Čimo et al 2020 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Exploitation of tolerance to drought stress in carrot (Daucus carota L.): an overview

Junaid,

Öztürk,

Gökçe

2023

Stress Biology

Self Cite

View full text Add to dashboard Cite

Drought stress is a significant environmental factor that adversely affects the growth and development of carrot (Daucus carota L.), resulting in reduced crop yields and quality. Drought stress induces a range of physiological and biochemical changes in carrots, including reduced germination, hindered cell elongation, wilting, and disrupted photosynthetic efficiency, ultimately leading to stunted growth and decreased root development. Recent research has focused on understanding the molecular mechanisms underlying carrot's response to drought stress, identifying key genes and transcription factors involved in drought tolerance. Transcriptomic and proteomic analyses have provided insights into the regulatory networks and signaling pathways involved in drought stress adaptation. Among biochemical processes, water scarcity alters carrot antioxidant levels, osmolytes, and hormones. This review provides an overview of the effects of drought stress on carrots and highlights recent advances in drought stress-related studies on this crop. Some recent advances in understanding the effects of drought stress on carrots and developing strategies for drought stress mitigation are crucial for ensuring sustainable carrot production in the face of changing climate conditions. However, understanding the mechanisms underlying the plant's response to drought stress is essential for developing strategies to improve its tolerance to water scarcity and ensure food security in regions affected by drought.

show abstract

Comparing vegetative growth patterns of cultivated (Daucus carota L. subsp. sativus) and wild carrots (Daucus carota L. subsp. carota) to eliminate genetic contamination from weed to crop

Godwin,

Pieralli,

Sofkova-Bobcheva

et al. 2024

Journal of Agriculture and Food Research

View full text Add to dashboard Cite

Investigation of drought induced biochemical and gene expression changes in carrot cultivars

Cited by 4 publications

References 44 publications

Physiological responses to drought stress of three pine species and comparative transcriptome analysis of Pinus yunnanensis var. pygmaea

Physiological responses to drought stress of three pine species and comparative transcriptome analysis of Pinus yunnanensis var. pygmaea

Exploitation of tolerance to drought stress in carrot (Daucus carota L.): an overview

Comparing vegetative growth patterns of cultivated (Daucus carota L. subsp. sativus) and wild carrots (Daucus carota L. subsp. carota) to eliminate genetic contamination from weed to crop

Contact Info

Product

Resources

About