Investigation of different cross-linking approaches on 3D gelatin scaffolds for tissue engineering application: A comparative analysis

Shankar, K. Gopal; Gostynska, Natalia; Montesi, Monica; Panseri, Silvia; Sprio, Simone; Kon, Elizaveta; Marcacci, Maurilio; Tampieri, Anna; Sandri, Monica

doi:10.1016/j.ijbiomac.2016.11.010

Cited by 58 publications

(55 citation statements)

References 38 publications

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“…Matrix degradability is influenced by the degree of cross-linking [ 38 ]. Previous studies have reported that the vacuum heating technique adds DHT cross-linking to gelatin sponges [ 39 ] and collagen sponges [ 40 ], attenuating biodegradability. In the present study, the degradability of vhEGCG[0.07]-GS was significantly lower than that of EGCG[0.07]-GS in distilled water ( Figure 1 D).…”

Section: Discussionmentioning

confidence: 99%

Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering

Honda

Takeda

et al. 2018

Molecules

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Chemical modification of gelatin using epigallocatechin gallate (EGCG) promotes bone formation in vivo. However, further improvements are required to increase the mechanical strength and bone-forming ability of fabricated EGCG-modified gelatin sponges (EGCG-GS) for practical applications in regenerative therapy. In the present study, we investigated whether vacuum heating-induced dehydrothermal cross-linking of EGCG-GS enhances bone formation in critical-sized rat calvarial defects. The bone-forming ability of vacuum-heated EGCG-GS (vhEGCG-GS) and other sponges was evaluated by micro-computed tomography and histological staining. The degradation of sponges was assessed using protein assays, and cell morphology and proliferation were verified by scanning electron microscopy and immunostaining using osteoblastic UMR106 cells in vitro. Four weeks after the implantation of sponges, greater bone formation was detected for vhEGCG-GS than for EGCG-GS or vacuum-heated gelatin sponges (dehydrothermal cross-linked sponges without EGCG). In vitro experiments revealed that the relatively low degradability of vhEGCG-GS supports cell attachment, proliferation, and cell–cell communication on the matrix. These findings suggest that vacuum heating enhanced the bone forming ability of EGCG-GS, possibly via the dehydrothermal cross-linking of EGCG-GS, which provides a scaffold for cells, and by maintaining the pharmacological effect of EGCG.

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Section: Discussionmentioning

confidence: 99%

Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering

Honda

Takeda

et al. 2018

Molecules

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“…Gelatin, a fibrous protein extracted from denatured native collagen (a major component of the ECM), shares a similar structure with native collagen. With good biocompatibility and low immunogenicity, gelatin has been extensively investigated in regenerating tissues such as synthetic polymer polycaprolactone, poly(lactic-co-glycolic acid) or crosslinking with chondroitin sulfate [ 23 , 24 , 25 , 26 ]. However, the super hydrophilicity and solubility of gelatin in turn bring high dissolubility [ 27 ].…”

Section: Introductionmentioning

confidence: 99%

Electrospun Zein/Gelatin Scaffold-Enhanced Cell Attachment and Growth of Human Periodontal Ligament Stem Cells

et al. 2017

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Periodontitis is a widespread dental disease affecting 10 to 15% of worldwide adult population, yet the current treatments are far from satisfactory. The human periodontal ligament stem cell is a promising potential seed cell population type in cell-based therapy and tissue regeneration, which require appropriate scaffold to provide a mimic extracellular matrix. Zein, a native protein derived from corn, has an excellent biodegradability, and therefore becomes a hotspot on research and application in the field of biomaterials. However, the high hydrophobicity of zein is unfavorable for cell adhesion and thus greatly limits its use. In this study, we fabricate co-electrospun zein/gelatin fiber scaffolds in order to take full advantages of the two natural materials and electrospun fiber structure. Zein and gelatin in four groups of different mass ratios (100:00, 100:20, 100:34, 100:50), and dissolved the mixtures in 1,1,1,3,3,3-hexafluoro-2-propanol, then produced membranes by electrospinning. The results showed that the scaffolds were smooth and homogeneous, as shown in scanning electron micrographs. The diameter of hybrid fibers was increased from 69 ± 22 nm to 950 ± 356 nm, with the proportion of gelatin increase. The cell affinity of zein/gelatin nanofibers was evaluated by using human periodontal ligament stem cells. The data showed that hydrophilicity and cytocompatibility of zein nanofibers were improved by blended gelatin. Taken together, our results indicated that the zein/gelatin co-electrospun fibers had sufficient mechanical properties, satisfied cytocompatibility, and can be utilized as biological scaffolds in the field of tissue regeneration.

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“…These results indicated that gelatin concentration, THC addition and heat treatment notable affected the pore size distribution of scaffolds. Thus, NH and HT 4% w/v scaffolds showed bigger pore size, which could explain lower deformation values [10], as shown by compression results.…”

Section: Mechanical and Morphological Characterisationmentioning

confidence: 77%

“…Therefore, porous gelatin scaffolds have found many applications in tissue engineering research, e.g. for bone, skin, cartilage and nerve regeneration [6][7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

Lactose-crosslinked fish gelatin-based porous scaffolds embedded with tetrahydrocurcumin for cartilage regeneration

Etxabide

Ribeiro

Ferreira

et al. 2018

International Journal of Biological Macromolecules

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Tetrahydrocurcumin (THC) is one of the major colourless metabolites of curcumin and shows even greater pharmacological and physiological benefits. The aim of this work was the manufacturing of porous scaffolds as a carrier of THC under physiological conditions. Fish-derived gelatin scaffolds were prepared by freeze-drying by two solutions concentrations (2.5% and 4% w/v), cross-linked via addition of lactose and heat-treated at 105 °C. This cross-linking reaction resulted in more water resistant scaffolds with a water uptake capacity higher than 800%. Along with the cross-linking reaction, the gelatin concentration affected the scaffold morphology, as observed by scanning electron microscopy images, by obtaining a reduced porosity but larger pores sizes when the initial gelatin concentration was increased. These morphological changes led to a scaffold's strength enhancement from 0.92 ± 0.22 MPa to 2.04 ± 0.18 MPa when gelatin concentration was increased. THC release slowed down when gelatin concentration increased from 2.5 to 4% w/v, showing a controlled profile within 96 h. Preliminary in vitro test with chondrocytes on scaffolds with 4% w/v gelatin offered higher metabolic activities and cell survival up to 14 days of incubation. Finally the addition of THC did not influence significantly the cytocompatibility and potential antibacterial properties were demonstrated successfully against Staphylococcus aureus.

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Investigation of different cross-linking approaches on 3D gelatin scaffolds for tissue engineering application: A comparative analysis

Cited by 58 publications

References 38 publications

Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering

Epigallocatechin Gallate-Modified Gelatin Sponges Treated by Vacuum Heating as a Novel Scaffold for Bone Tissue Engineering

Electrospun Zein/Gelatin Scaffold-Enhanced Cell Attachment and Growth of Human Periodontal Ligament Stem Cells

Lactose-crosslinked fish gelatin-based porous scaffolds embedded with tetrahydrocurcumin for cartilage regeneration

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