Investigation of differences in follicular penetration of particle-and nonparticle-containing emulsions by laser scanning microscopy

Ossadnik, Michael; Richter, Heike; Teichmann, A.; Koch, Stefan; Schäfer, U.; Wepf, Roger; Sterry, Wolfram; Lademann, Jürgen

doi:10.1134/s1054660x06050033

Cited by 44 publications

(27 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…25 The e±cient penetration of particles sized approximately 600 nm could be explained by examining the hair surface structure. 26 Figure 5 shows the cuticles of a hair shaft. The cuticle thickness amounts to approximately 600 nm.…”

Section: Optimal Particle Size For Penetration Into the Hair Folliclesmentioning

confidence: 99%

Hair follicles as a target structure for nanoparticles

Lademann

Knorr

Richter

et al. 2015

J. Innov. Opt. Health Sci.

Self Cite

View full text Add to dashboard Cite

For at least two decades, nanoparticles have been investigated for their capability to deliver topically applied substances through the skin barrier. Based on¯ndings that nanoparticles are highly suitable for penetrating the blood-brain barrier, their use for drug delivery through the skin has become a topic of intense research. In spite of the research e®orts by academia and industry, a commercial product permitting the nanoparticle-assisted delivery of topically applied drugs has not yet been developed. However, nanoparticles of approximately 600 nm in diameter have been shown to penetrate e±ciently into the hair follicles, where they can be stored for several days. The successful loading of nanoparticles with drugs and their triggered release inside the hair follicle may present an ideal method for localized drug delivery. Depending on the particle size, such a method would permit targeting speci¯c structures in the hair follicles such as stem cells or immune cells or blood vessels found in the vicinity of the hair follicles.

show abstract

Section: Optimal Particle Size For Penetration Into the Hair Folliclesmentioning

confidence: 99%

Hair follicles as a target structure for nanoparticles

Lademann

Knorr

Richter

et al. 2015

J. Innov. Opt. Health Sci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…9 Therefore, pig skin was extensively used as a surrogate for HS for testing nanoparticle penetration, especially when follicular penetration is in scope. [10][11][12] Artificially reconstructed human skin or "skin equivalents" (SE) represent another alternative skin model for conducting penetration/permeation experiments.…”

Section: Introductionmentioning

confidence: 99%

Setup for investigating gold nanoparticle penetration through reconstructed skin and comparison to published human skin data

Thude

Schneider

2012

J. Biomed. Opt

View full text Add to dashboard Cite

This is an by copyright after embargo allowed publisher's PDF of an article published in Labouta, H.I., Thude, S., Schneider, M. Setup for investigating gold nanoparticle penetration through reconstructed skin and comparison to published human skin data (2013) Journal of Biomedical Optics, 18 (6), art. no. 61218.Setup for investigating gold nanoparticle penetration through reconstructed skin and comparison to published human skin data Abstract. Owing to the limited source of human skin (HS) and the ethical restrictions of using animals in experiments, in vitro skin equivalents are a possible alternative for conducting particle penetration experiments. The conditions for conducting penetration experiments with model particles, 15-nm gold nanoparticles (AuNP), through nonsealed skin equivalents are described for the first time. These conditions include experimental setup, sterility conditions, effective applied dose determination, skin sectioning, and skin integrity check. Penetration at different exposure times (two and 24 h) and after tissue fixation (fixed versus unfixed skin) are examined to establish a benchmark in comparison to HS in an attempt to get similar results to HS experiments presented earlier. Multiphoton microscopy is used to detect gold luminescence in skin sections. λ ex ¼ 800 nm is used for excitation of AuNP and skin samples, allowing us to determine a relative index for particle penetration. Despite the observed overpredictability of penetration into skin equivalents, they could serve as a first fast screen for testing the behavior of nanoparticles and extrapolate their penetration behavior into HS. Further investigations are required to test a wide range of particles of different physicochemical properties to validate the skin equivalent-human skin particle penetration relationship.

show abstract

“…Massage facilitates the activation of the pumping mechanism of hair movement that has been proposed as a mechanism for follicular deposition [23, 24, 29-31]. HE images of skin before and after 2-min massage showed that this treatment protocol did not cause any physical damage to the skin barrier.…”

Section: Resultsmentioning

confidence: 93%

Evaluation of Quantum Dot Skin Penetration in Porcine Skin: Effect of Age and Anatomical Site of Topical Application

Nastiti

Mohammed

Telaprolu

et al. 2019

Skin Pharmacol Physiol

View full text Add to dashboard Cite

Background: Pig skin is a widely acknowledged surrogate for human skin for in vitro/ex vivo skin penetration studies with application for small molecules and nanosystems. We have investigated the influence of biological factors such as age and anatomical site on the penetration and distribution of nanoparticles (2.1 nm hydrophilic CdTe/CdS quantum dots: QDs) in adult pig skin (APS), weanling pig skin (WPS) and newborn pig skin (NBPS) at two different anatomical sites (ear and abdomen). Methods: QDs in saline were applied to 1 × 1 cm² skin (62.5 pmol/cm²) with 2-min finger rubbing using a standardized protocol. After 6- or 24-h incubation on Franz diffusion cells, tape stripping (×10) followed by manual follicular casting was conducted. Cadmium in QDs was quantified using inductively coupled plasma mass spectrometry for all samples. The presence of QDs in similarly treated skin samples was also captured using multiphoton tomography. Results: QDs were mainly localized in hair follicles after 6 and 24 h of exposure with no cadmium detected in the Franz cell receptor compartment regardless of pig age or anatomical site. The amount of QDs deposited in the follicles was similar at 6 h but higher on APS and WPS ears compared to NBPS ears at 24 h. This is associated with the high follicle density and small follicle diameter of the NBPS compared to the smaller density of much larger follicles on the APS. NBPS showed consistent QD distribution for ear and abdomen up to 24 h. Conclusions: There is minimal penetration of QDs through pig skin. Density and diameter of follicles in association with age of pigs and application site influenced the amount of QDs deposited in follicles. The structure of the stratum corneum, follicle density and diameter of NBPS are similar to human skin suggesting that NBPS is an appropriate model for human skin in the evaluation of topical applications of a range of chemicals including nanosystems.

show abstract

Investigation of differences in follicular penetration of particle-and nonparticle-containing emulsions by laser scanning microscopy

Cited by 44 publications

References 11 publications

Hair follicles as a target structure for nanoparticles

Hair follicles as a target structure for nanoparticles

Setup for investigating gold nanoparticle penetration through reconstructed skin and comparison to published human skin data

Evaluation of Quantum Dot Skin Penetration in Porcine Skin: Effect of Age and Anatomical Site of Topical Application

Contact Info

Product

Resources

About