Investigation of defense response and immune priming in Biomphalaria glabrata and Biomphalaria straminea, two species with different susceptibility to Schistosoma mansoni

Melo, Elverson Soares de; Brayner, Fábio André; Portela, Nairomberg Cavalcanti; França, Iany Raissa Silva; Alves, Luíz Carlos

doi:10.1007/s00436-019-06495-4

Cited by 19 publications

(9 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Following this early THC reduction, a significant increase at the 1 st DPO in both groups was detected especially in the susceptible group. This finding was in agreement with de Melo et al [32] , who stated that the response at the 1 st DPO was more potent than the response during earlier stages of host-parasite interaction. According to Araque et al [33] , the early migration of hemocytes to the internal tissues happens regardless of snail-parasite compatibility status.…”

Section: Discussionsupporting

confidence: 93%

Morphology and kinetics of susceptible and resistant Biomphalaria alexandrina hemocytes during the first week of exposure to Schistosoma mansoni miracidia

Khedr

2020

Parasitologists United Journal

View full text Add to dashboard Cite

Background: Few studies concerning Biomphalaria alexandrina (B. alexandrina) snail hemocytes' subpopulations, and their relation to the compatibility with Schistosoma mansoni (S. mansoni)' miracidia were performed. Manipulation of parasite development inside these snails could be applied as a control measure against schistosomiasis. Objectives: Knowing that the snail hemocytes temporarily bind to the parasites, allowing the development of cercariae that are infective to the definitive host. This study aimed at studying the morphology and kinetics of diverse hemocytes of susceptible and resistant B. alexandrina and their participation in the snail early immune response after challenge by S. mansoni. Material and Methods: Giemsa stained hemocytes were characterized using light microscopy. Total and differential hemocyte counts (THC and DHC) were calculated in the hemolymph of two groups composed of 60 susceptible and 60 resistant snails. Each group was further subdivided as 12 control pre-exposure snails (PE) and 48 post-exposure snails (PO) to S. mansoni at different time points (6 h, 1, 3 and 7 days). THC and DHC counts were recorded by a snail hemogram. Results: Results revealed that granulocytes constituted the most common population all through the experiment with the large dense-granulated granulocytes subpopulation being the largest-sized cells detected. The highly reactive subpopulations that increased in number upon exposure to S. mansoni were the few-granulated and the large-granulated granulocytes, suggesting their possible participation in early parasite destruction. Conclusion:The resulting hemograms helped determine the participation of hemocyte populations and subpopulations in the defense against S. mansoni, aiding in understanding snail compatibility patterns. Further studies to propagate transgenic B. alexandrina snails abundant in large granular granulocytes utilizing (gene editing) CRISPR-Cas9 technique are recommended. This would be required to spread schistosome resistance traits in snail populations, thus, contributing to reduced schistosomiasis transmission in the long run.

show abstract

Section: Discussionsupporting

confidence: 93%

Morphology and kinetics of susceptible and resistant Biomphalaria alexandrina hemocytes during the first week of exposure to Schistosoma mansoni miracidia

Khedr

2020

Parasitologists United Journal

View full text Add to dashboard Cite

show abstract

“…Studies have reported that specific primed immune responses of Drosophila ( 19 ) and silkworm ( 18 , 62 ) were dependent on phagocytes. In mollusks, the total hemocyte count and phagocytic activity were increased after secondary infection in snails ( 63 ), scallop ( 36 ) and oysters ( 20 , 64 ). In the present study, we demonstrated the importance of phagocytosis from a molecular perspective, as some genes in CL1 involved in lysosome, phagosome, endocytosis and Fc gamma R-mediated phagocytosis had higher expression levels in secondary infection.…”

Section: Discussionmentioning

confidence: 99%

The Enhanced Immune Protection in Small Abalone Haliotis diversicolor Against a Secondary Infection With Vibrio harveyi

Tuo

Bai

et al. 2021

Front. Immunol.

View full text Add to dashboard Cite

In recent years, more and more studies have shown that early pathogenic bacterial infection in invertebrates can enhance immunity and significantly reduce mortality when reinfected with the same pathogen. There are mechanisms to explain this phenomenon, but they are relatively few. In addition, dose-dependent primary infection is also associated with increased immunity. In the present study, the initial infection dose and mortality of abalone Haliotis diversicolor after reinfection with Vibrio harveyi were recorded, and the mechanism of immune enhancement was investigated by the transcriptomic response of abalone after two successive stimuli with V. harveyi. Priming with different concentrations of pathogen can enhance immunity; however, higher concentration is not always better. Compared with the first exposure, more genes were up-regulated after the second exposure. Among the commonly expressed genes, the immune related genes were significantly or persistently highly expressed after two infections and included pattern recognition receptors as well as immune effectors, such as toll-like receptors, perlucin 4, scavenger receptor class B-like protein, cytochrome P450 1B1-like, glutathione S-transferase 6, lysozyme and so on; in addition, these immune-related genes were mainly distributed in the pathways related to phagocytosis and calcium signaling. Among the specifically expressed genes, compared with the first infection, more genes were involved in the immune, metabolic and digestive pathways after the second infection, which would be more conducive to preventing the invasion of pathogens. This study outlined the mechanism of immune enhancement in abalone after secondary infection at the global molecular level, which is helpful for a comprehensive understanding of the mechanism of immune priming in invertebrates.

show abstract

“…It is reported that immune protection after identical stimulation is significantly stronger in invertebrates than that in an unvaccinated group and that the effect is similar to that of a vaccine (3,37,38,(54)(55)(56). A mechanism of enhanced immune protection is demonstrated, but it is also reported to be associated with several factors: the interaction between different hosts and pathogens, the induced dose, and the duration (57).…”

Section: Discussionmentioning

confidence: 99%

“…Dubief and colleagues demonstrate that the hemolymph phagocytosis of Haliotis tuberculata is enhanced after reinfection by Vibrio harveyi (37). De Melo and colleagues demonstrate that secondary infection by Schistosoma japonicum caused a significant increase in the number of granulocytes in hemolymph in B. glabrata and Biomphalaria straminea (38). Research into Eriocheir sinensis indicates that the survival rate of individuals immunized with inactivated Aeromonas hydrophila for 7 days was significantly higher than that of untreated individuals when they were infected with live A. hydrophila.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Immune Protection of Mud Crab Scylla paramamosain in Response to the Secondary Challenge by Vibrio parahaemolyticus

et al. 2020

View full text Add to dashboard Cite

Investigation of defense response and immune priming in Biomphalaria glabrata and Biomphalaria straminea, two species with different susceptibility to Schistosoma mansoni

Cited by 19 publications

References 64 publications

Morphology and kinetics of susceptible and resistant Biomphalaria alexandrina hemocytes during the first week of exposure to Schistosoma mansoni miracidia

Morphology and kinetics of susceptible and resistant Biomphalaria alexandrina hemocytes during the first week of exposure to Schistosoma mansoni miracidia

The Enhanced Immune Protection in Small Abalone Haliotis diversicolor Against a Secondary Infection With Vibrio harveyi

Enhanced Immune Protection of Mud Crab Scylla paramamosain in Response to the Secondary Challenge by Vibrio parahaemolyticus

Contact Info

Product

Resources

About