Investigation of conception rates achieved with the transfer of sexed and unsexed bovine embryos

Karaşahi̇n, Tahir; Akyol, Numan; Satilmiş, Muharrem; Kizil, Sedat Hamdi; Bucak, Mustafa Numan; Çoyan, Kenan

doi:10.3906/vet-1310-22

Cited by 3 publications

(2 citation statements)

References 20 publications

(31 reference statements)

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“…Prior to flushing, each cow received epidural anesthesia (5 to 7 mL of 2% lidocaine; Vilcain, Vilsan, Türkiye). Ova/ embryos were collected non-surgically 6.5 to 7 days after the first TAI using lactated Ringer's solution containing 1% calf serum (CS, N4762; Sigma-Aldrich, USA) and 125 mg/L of kanamycin (Kanovet, Vetaş, Türkiye) [14,15] . Each uterine horn was flushed with 800 to 1000 mL of the solution.…”

Section: Ova/embryo Collectionmentioning

confidence: 99%

Laktasyodaki Simental İneklerde Follikül Aspirasyonu Zamanında Tek Bir Ultrason Muayenesi ile Tahmin Edilen Dominant Follikül Varlığı veya Yokluğunun Süperovulatör Cevaplar ve Embriyo Üretimi Üzerindeki Etkileri

Cirit¹,

Özmen²,

Köse³

et al. 2019

Kafkas Univ Vet Fak Derg

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The aim of the study was to evaluate effects of presence or absence of a dominant follicle (DF) estimated by a single ultrasound examination at the time of follicular aspiration (FA) on superstimulatory and superovulatory responses and embryo production in lactating Simmental cows. At random stages of the estrous cycle, the ovaries of cows (n=42) were examined by transrectal ultrasonography (US) and all follicles ≥3 mm were counted. Donors with <10 follicles 3-8 mm in diameter were considered to have a dominant follicle (group DF+; n=30), while donors ≥10 small follicles 3-8 mm were classified as having no dominant follicle (group DF-; n=12). Just after US examination, all cows were subjected to ultrasoundguided transvaginal aspiration of all follicles ≥5 mm and a progesterone-releasing device was placed in the vagina. Thirty-six h after FA, all cows were superstimulated with FSH, which was given as twice-daily injections over 6 days. Cows were pre-treated with a single dose of 400 IU of eCG 24 h before the start of FSH treatments. It was concluded from this study that the presence of a DF estimated by a single ultrasound examination at the time of FA effects negatively the superstimulatory and superovulatory responses, fertilization rate and embryo quality (P<0.05) but not the number of embryos collected. It was also concluded that estimation of a dominant follicle by a single ultrasound examination at the time of follicular aspiration based on the number of small follicles may be used the selection of potential donor cows and can significantly contribute to improvements in superstimulatory and superovulatory responses and embryo quality. ÖzBu çalışmanın amacı, laktasyondaki Simental ineklerde follikül aspirasyonu (FA) zamanında tek bir ultrason muayenesi ile saptanan dominant follikül (DF) varlığı veya yokluğunun süperstimülatör ve süperovulatör cevaplar ve embriyo üretimi üzerindeki etkilerinin değerlendirilmesiydi. Östrus siklusunun tesadüfi aşamalarında, ineklerin (n=42) ovaryumları ultrason (US) ile muayene edilerek ≥3 mm tüm folliküller sayıldı. Üç-8 mm çapında <10 küçük follikülü olan inekler DF'ü var olarak (DF+ grubu; n=30), ≥10 küçük follikülü olan inekler ise DF'ü yok olarak (DF-grubu; n=12) sınıflandırıldı. Ultrason muayenesinden hemen sonra tüm ineklerin ≥5 mm tüm follikülleri aspire edildi ve vajina içine bir progesteron salıverici araç yerleştirildi. Tüm ineklere, follikül aspirasyonundan 36 saat sonrasından başlanarak 6 gün boyunca günde 2 kez FSH uygulandı. İneklere FSH uygulamasının başlamasından 24 saat önce 400 IU tek doz eCG ile ön tedavi uygulandı. Çalışmadan, FA günü tek bir ultrason muayenesi ile tahmin edilen DF varlığının süperstimülatör ve superovulatör yanıtları, fertilizasyon oranını ve embriyo kalitesini olumsuz etkilediği (P<0.05), fakat toplanan embriyo sayısını etkilemediği sonucuna varıldı. Çalışmadan ayrıca, FA günü yapılan tek bir ultrason muayenesinde küçük folliküllerin sayısına göre DF varlığının tahmin edilmesi işleminin potansiyel vericilerin seçiminde kullanılabileceği v...

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Section: Ova/embryo Collectionmentioning

confidence: 99%

Laktasyodaki Simental İneklerde Follikül Aspirasyonu Zamanında Tek Bir Ultrason Muayenesi ile Tahmin Edilen Dominant Follikül Varlığı veya Yokluğunun Süperovulatör Cevaplar ve Embriyo Üretimi Üzerindeki Etkileri

Cirit¹,

Özmen²,

Köse³

et al. 2019

Kafkas Univ Vet Fak Derg

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“…About 80% (355,205 embryos) were produced in Brazil and 17% were produced in North America (USA and Canada). While the use of IVF has been increasing greatly around the world, especially in countries such as the United States, Brazil, and Argentina, it is still in the research phase, which is only gradually increasing in Turkey (24,32). Birler et al (33) demonstrated the first IVFproduced healthy live-born lambs at İstanbul University in 2000.…”

Section: Ivf and Embryo Culture (Ivc)mentioning

confidence: 99%

Application of reproductive biotechnologies for sustainable production of livestock in Turkey

2018

Turk J Vet Anim Sci

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The majority of farming households in Turkey still depend on low-input semisubsistence agriculture and livestock production. Despite a slow decline in recent years, agriculture and livestock production remain major employers in Turkey and are significant contributors to the country's gross domestic product (GDP) (1). Livestock production is an important and traditional sector in the agricultural economy of Turkey and is facing a decrease in herd numbers of cattle, sheep, and goats, and in meat production, despite growth in domestic demand. Animal production is vital for sufficient and balanced nutrition of the population. Sound evaluation of the critical factors of production, namely capital and labor, are necessary for establishing the baseline and advancing animal science and technology. The livestock industry is also valuable for enabling strategic investments and placements of labor to develop within the sector. The livestock industry is linked with crop production and agricultural economy.The employment rate in animal agriculture is much higher in Turkey than in the USA and EU. The livestock industry helps to reduce unemployment year-round. According to TurkStat, the agriculture sector constituted 7.4% of the total GDP in Turkey (1). However, agriculture is only 1.7% and 1.9% of the total GDP in the USA and EU, respectively (1). In its contribution to the economy, the Turkish agricultural sector consists of three million agricultural farms, most of which typically have mixed farming in which crops and animal production occur together. According to typological classification, about two-thirds of farms specialize in field crop production and livestock production. Only 2%-3% of the total are specialized in livestock production alone (2,3). Although this percentage is low, in recent years there has been intensive investments in livestock by means of low interest rate loans and government subsidies. Livestock production is important, especially milk production, for which Turkey ranks about 15 th in the world.Despite the fluctuations in milk and meat prices, the livestock industry has a significant role in preventing Abstract: Reproductive biotechnology encompasses powerful tools to enhance the efficiency and profitability of livestock reproduction, production, and product quality. These technologies include genomic selection, evaluation of semen (from bulls, bucks, and rams) using advanced cell and molecular technologies, semen cryopreservation and artificial insemination, estrus synchronization, superovulation of the females (cows, dams, and ewes), ovum pick-up, in vitro fertilization and embryo culture, embryo transfer, and pregnancy detection. The extent of applications of these modern technologies and their economic impacts for livestock production in Turkey are elusive. As a result, there is an urgent need for sound economic analyses, research and education, training of animal producers to facilitate technology transfer, and informing of the public of the benefits of animal biotechnology. The objective of th...

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Effect of the interval from follicle aspiration to initiation of lengthened FSH treatment on follicular superstimulatory and superovulatory responses and embryo production in lactating Simmental cows

et al. 2019

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Investigation of conception rates achieved with the transfer of sexed and unsexed bovine embryos

Cited by 3 publications

References 20 publications

Laktasyodaki Simental İneklerde Follikül Aspirasyonu Zamanında Tek Bir Ultrason Muayenesi ile Tahmin Edilen Dominant Follikül Varlığı veya Yokluğunun Süperovulatör Cevaplar ve Embriyo Üretimi Üzerindeki Etkileri

Laktasyodaki Simental İneklerde Follikül Aspirasyonu Zamanında Tek Bir Ultrason Muayenesi ile Tahmin Edilen Dominant Follikül Varlığı veya Yokluğunun Süperovulatör Cevaplar ve Embriyo Üretimi Üzerindeki Etkileri

Application of reproductive biotechnologies for sustainable production of livestock in Turkey

Effect of the interval from follicle aspiration to initiation of lengthened FSH treatment on follicular superstimulatory and superovulatory responses and embryo production in lactating Simmental cows

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