Investigation of chitin recovery from shrimp waste by yeast fermentation

Abstract

This study investigated the possibility of using yeast fermentation to transform shrimp waste to chitin for further application. The white leg shrimp head was incubated with three yeast strains Yarrowia lipolytica, Candida tropicalis and Pichia kudriavzevii, in comparison with bacteria Bacillus subtilis and commercial protease Alcalase. The efficacy of fermentation was evaluated through deproteinization and demineralization levels after 2 days. A deproteinization of 80.9% was obtained when incubation with Y. lipolytica which was significantly higher than 76.9% and 65.6% obtained with Alcalase hydrolysis and B. subtilis incubation respectively. Besides, C. tropicalis and P. kudriavzevii expressed a similar low level on deproteinization (31.3-31.7%.) All three yeast showed a good demineralization in range 38.2-49.4% on shrimp head while B. subtilis could demineralize only 16.0%. This primary research shows a potential application of yeast fermentation in chitin recovery from shrimp waste.

Efficient Chitin Extraction from Shrimp Exoskeletons through Single-Step Fermentation by Pseudomonas aeruginosa QF50 and Serratia sp. QCS23

“…QCS23 also exhibited an increase in demineralization efficiency (21.20 ± 0.78%) with increasing glucose concentrations, indicating that while it may be less efficient at lower glucose levels, its demineralization capability is significantly enhanced at higher concentrations. These results exceed those reported by Ta et al [37] who achieved an 80.9% deproteinization after two days of incubation using Yarrowia lipolytica and achieved a demineralization range of 38.2-49.4% with shrimp head waste. In contrast, Gharibzadeh et al [38] reached a maximum value of 91.22% deproteinization using Bacillus licheniformis at 45 • C and 96.55% demineralization using Lactobacillus plantarum at 37 • C, both during a single-step process.…”