Investigation of Changes in Spermatozoon Characteristics, Chromatin Structure, and Antioxidant/Oxidant Parameters after Freeze-Thawing of Hesperidin (Vitamin P) Doses Added to Ram Semen

Yeni, Deniz; Güngör, Salim; Avdatek, Fatih; Gülhan, Mehmet Fuat; Olğaç, Kemal Tuna; ̇İnanç, Muhammed Enes; Denk, Barış; Taşdemir, Umut

doi:10.3390/life12111780

Cited by 6 publications

(10 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, the freezing process negatively affects spermatological parameters and fertility (Kulaksız and Daşkın 2009). Due to all these factors, researchers focused on adding substances with high antioxidant properties to extenders during semen freezing (Yeni et al 2022). Capsaicin (8-methyl-N-vanilyl-6-nonenamide) is an active ingredient responsible for the pungency of hot peppers.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Ram Semen Using Capsaicin Supplemented Tris Extender

̇İnanç

Avdatek

Güngör

et al. 2023

Kocatepe Veterinary Journal

View full text Add to dashboard Cite

In this study, it was designed to reveal the effects of capsaicin on oxidative stress and freezability of ram semen. Ejaculates were taken from Sönmez rams and divided into five specimens and diluted with extender at different rates (4 mM, 2 mM, 1 mM, 500 µM) with and without capsaicin (control; C). At the end of the study, sperm motility and kinetic parameters, plasma membrane acrosome integrity (PMAI), mitochondrial membrane potential (MMP), DNA damage, oxidant and antioxidant parameters were analyzed. A decrease was observed in the groups containing capsaicin compared to the C in terms of progressive, total motility and kinetic parameters (p

show abstract

Section: Introductionmentioning

confidence: 99%

Cryopreservation of Ram Semen Using Capsaicin Supplemented Tris Extender

̇İnanç

Avdatek

Güngör

et al. 2023

Kocatepe Veterinary Journal

View full text Add to dashboard Cite

show abstract

“…Supporting the DNA integrity result identified in the JB25 and JB50 groups in this study, it was demonstrated that 2, 4 and 6 μg/ml spirulina extract had a protective effect on spermatozoon DNA integrity, and a toxic effect when > 6 μg/ml of it was used (Mizera et al 2019). The findings of this study were in favour of the findings of the studies conducted by our researcher group in recent years, where we have used various antioxidants (rosmarinic acid, pine bark tree extract, vaccenic acid, hesperidin, thymoquinone) due to their remedial effects on DNA integrity (Yeni et al 2018;Taşdemir et al 2020;Avdatek et al 2022;İnanç et al 2022;Yeni et al 2022). It was concluded that as an antioxidant, JB at appropriate doses (25 and 50 μg/ml) maintains the integrity of DNA and this antioxidant property of JB manifests its healing effect on DNA damage by creating an additive effect along with other cryoprotectants in the extender.…”

Section: Discussionmentioning

confidence: 52%

Evaluation of the in vitro cryopreservative performance of Juniper berry oil (Juniperus communis) on frozen-thawed bull semen

Güngör,

Yeni,

İnanç

et al. 2023

Acta Vet. Brno

Self Cite

View full text Add to dashboard Cite

This study aimed to reveal the effects of juniper (Juniperus communis) berry oil (JB) when added to the Tris extender, on bull (Holstein) sperm motility characteristics, abnormal sperm ratio, DNA fragmentation and some oxidative stress markers. The pooled ejaculate was divided into five equal parts as control (C) and Tris diluent containing 25, 50, 100, 200 μg/ml JB. Diluted semen was incubated at 4 °C for 4 h and then frozen in liquid nitrogen (−196 °C). After freezing-thawing, motility characteristics were determined using a sperm analyzer system. The results showed that the 25 μg/ml dose of JB had an improving effect on overall sperm motility (P < 0.05). The total abnormal spermatozoa ratio was not significantly different between the groups (P > 0.05). The JB25 and JB50 groups had higher DNA integrity than the other groups (P < 0.05). This finding was supported by low malondialdehyde levels (P < 0.05). Especially the 25 μg/ml dose of JB was found to have a remedial effect on DNA integrity, as well as increasing progressive and total motility rates. This study has revealed new information on the effects of JB on bull semen cryopreservation. Juniper berry oil may be used as an antioxidant in bull semen freezing.

show abstract

“…However, an excess concentration of ROS during the semen freezing process causes oxidative stress (Gao et al, 2007). The resulting oxidative stress brings with it undesirable effects that may even include the loss of motility in the sperm cell (Yeni et al, 2022). In this study, it was determined that all doses of SA, except for the 0.5 mM dose, had an improving effect on progressive and total motility.…”

Section: Discussionmentioning

confidence: 99%

Investigation of the effects of syringic acid supplemented to Tris semen diluent on ram semen freezability

Avdatek

̇İnanç

Gülhan

et al. 2023

Reprod Domestic Animals

Self Cite

View full text Add to dashboard Cite

To the best of our knowledge, no research has been conducted to test the effects of syringic acid (SA) on ram semen freezing within the scope of natural antioxidants added to semen extenders. Therefore, this study had two main objectives. First, to test whether adding SA to ram semen freezing extender has a protective effect and contributes positively to sperm kinetic, plasma and acrosome integrity, mitochondrial membrane potential, lipid peroxidation, oxidant and antioxidant and DNA damage parameters after thawing. Second, it was to determine at what concentration the SA supplemented to the extender could be applied by in vitro studies by preserving the fertilization ability of frozen semen at the highest level. In the study, six individuals of Sönmez rams were used. The semen was collected from the rams using an artificial vagina and pooled. The pooled semen was divided into five different groups and extended with 0, 0.5, 1, 2 and 4 mM SA (control C, SA0.5, SA1, SA2 and SA4, respectively). After dilution, the semen samples were kept at 4°C for 3 h, then loaded into 0.25 mL straws and frozen in liquid nitrogen vapour. The SA1 and SA2 groups were higher plasma membrane and acrosome integrity (PMAI), high mitochondrial membrane potential (HMMP), plasma membrane integrity and motility compared to other groups (p < .05). It was observed that SA supplemented to the Tris extender significantly reduced DNA damage, and the lowest values were obtained especially in the SA1 and SA2 treatments (p < .05). Also, lowest MDA level was determined at the SA1 and this was statistically significant compared to SA4 and C (p < .05). In conclusion, it was revealed that SA added to Tris semen extender at 1 and 2 mM treatment doses increased progressive and total motility and preserved PMAI, plasma membrane integrity, HMMP and DNA integrity.

show abstract

Investigation of Changes in Spermatozoon Characteristics, Chromatin Structure, and Antioxidant/Oxidant Parameters after Freeze-Thawing of Hesperidin (Vitamin P) Doses Added to Ram Semen

Cited by 6 publications

References 54 publications

Cryopreservation of Ram Semen Using Capsaicin Supplemented Tris Extender

Cryopreservation of Ram Semen Using Capsaicin Supplemented Tris Extender

Evaluation of the in vitro cryopreservative performance of Juniper berry oil (Juniperus communis) on frozen-thawed bull semen

Investigation of the effects of syringic acid supplemented to Tris semen diluent on ram semen freezability

Contact Info

Product

Resources

About