Investigation of cellular responses upon interaction with silver nanoparticles

An, Seong Soo A.; Subbiah, Ramesh; Jeon, Seongbeom; Yun, Kyusik; Ahn, Sang Jung

doi:10.2147/ijn.s88508

Cited by 9 publications

(12 citation statements)

References 36 publications

(51 reference statements)

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“…In this work, we found that a typical model of alveolar epithelial cell exposed to Fe 2 O 3 and TiO 2 NPs presented a reduction in cell stiffness. From previous studies, Subbiah et al (2015) reported an increase in cell stiffness after exposure to silver NPs in several cell types including A549 cells, human bone marrow stromal cells (H-S5) and breast cancer cells (NIH3T3). However, the dose employed by these authors was the highest tested here (40 μg/mL of NPs), which presented clear cytotoxic effects on all the three types of cells used.…”

Section: Discussionmentioning

confidence: 99%

Biomechanical Response of Lung Epithelial Cells to Iron Oxide and Titanium Dioxide Nanoparticles

et al. 2019

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Increasing evidence shows that lungs can be damaged by inhalation of nanoparticles (NPs) at environmental and occupational settings. Recent findings have associated the exposure to iron oxide (Fe 2 O 3 ) and titanium dioxide (TiO 2 ) – NPs widely used in biomedical and clinical research – with pulmonary oxidative stress and inflammation. Although changes on cellular mechanics could contribute to pulmonary inflammation, there is no information regarding the effects of Fe 2 O 3 and TiO 2 on alveolar epithelial cell biomechanics. The aim was to investigate the NPs-induced biomechanical effects in terms of cell stiffness and traction forces exerted by human alveolar epithelial cells. Cell Young’s modulus ( E ) measured by atomic force microscopy in alveolar epithelial cells significantly decreased after exposure to Fe 2 O 3 and TiO 2 (∼28 and ∼25%, respectively) compared to control conditions. Moreover, both NPs induced a similar reduction in the traction forces exerted by the alveolar epithelial cells in comparison to the control conditions. Accordingly, immunofluorescence images revealed a reduction of actomyosin stress fibers in response to the exposure to NPs. However, no inflammatory response was detected. In conclusion, an acute exposure of epithelial pulmonary cells to Fe 2 O 3 and TiO 2 NPs, which was mild since it was non-cytotoxic and did not induce inflammation, modified cell biomechanical properties which could be translated into damage of the epithelial barrier integrity, suggesting that mild environmental inhalation of Fe 2 O 3 and TiO 2 NPs could not be innocuous.

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Section: Discussionmentioning

confidence: 99%

Biomechanical Response of Lung Epithelial Cells to Iron Oxide and Titanium Dioxide Nanoparticles

et al. 2019

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“…Thus, AFM is a powerful tool to analyze the interaction between the cells and AgNPs at high accuracy. In this respect, Subbiah et al employed AFM to investigate the physicomechanical responses of A549, human bone marrow stromal cells (HS-5) and mouse fibroblasts (NIH3T3) exposed to AgNPs [226]. Bioassays (CCK-8, GSH, and lipid peroxidation) were also concurrently performed.…”

Section: In Vitro Cell Cultivationmentioning

confidence: 99%

“…NP: nanoparticles; Y. M.: Young’s modulus. Reproduced from [226] with permission of Dove Medical Press Ltd.…”

Section: Figurementioning

confidence: 99%

Bactericidal and Cytotoxic Properties of Silver Nanoparticles

Liao

Tjong

2019

IJMS

698

436

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Silver nanoparticles (AgNPs) can be synthesized from a variety of techniques including physical, chemical and biological routes. They have been widely used as nanomaterials for manufacturing cosmetic and healthcare products, antimicrobial textiles, wound dressings, antitumor drug carriers, etc. due to their excellent antimicrobial properties. Accordingly, AgNPs have gained access into our daily life, and the inevitable human exposure to these nanoparticles has raised concerns about their potential hazards to the environment, health, and safety in recent years. From in vitro cell cultivation tests, AgNPs have been reported to be toxic to several human cell lines including human bronchial epithelial cells, human umbilical vein endothelial cells, red blood cells, human peripheral blood mononuclear cells, immortal human keratinocytes, liver cells, etc. AgNPs induce a dose-, size- and time-dependent cytotoxicity, particularly for those with sizes ≤10 nm. Furthermore, AgNPs can cross the brain blood barrier of mice through the circulation system on the basis of in vivo animal tests. AgNPs tend to accumulate in mice organs such as liver, spleen, kidney and brain following intravenous, intraperitoneal, and intratracheal routes of administration. In this respect, AgNPs are considered a double-edged sword that can eliminate microorganisms but induce cytotoxicity in mammalian cells. This article provides a state-of-the-art review on the synthesis of AgNPs, and their applications in antimicrobial textile fabrics, food packaging films, and wound dressings. Particular attention is paid to the bactericidal activity and cytotoxic effect in mammalian cells.

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“…In addition, the type of reducing agents used for the synthesis of AgNPs is a crucial factor for the determination of cytotoxicity. 1 , 26 Dissolved Ag cations are biochemically active agents that interfere with bacterial cell membrane permeability and cellular metabolism. Ag also contributes to the formation of reactive oxygen species and other mechanisms that potentially affect prokaryotic cells.…”

Section: Discussionmentioning

confidence: 99%

<p>Beneficial Effect of TaON-Ag Nanocomposite Titanium on Antibacterial Capacity in Orthopedic Application</p>

Chang

et al. 2020

IJN

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In this study, a novel oxygenated nanocomposite thin film, TaON-Ag, was investigated in vitro and in vivo to evaluate its biocompatibility and antibacterial ability. Material and Methods: The antibacterial ability of TaON-Ag nanocomposite-coated titanium (Ti) was evaluated using the Kirby-Bauer disk diffusion susceptibility test. The effects of TaON-Ag nanocomposite-coated metal on osteogenesis were further evaluated in an in vitro osteogenic culture model with rat marrow-derived mesenchymal stem cells (rMSCs). Furthermore, titanium rods coated with TaON-Ag were implanted into a rat femur fracture model either with or without osteomyelitis to investigate the effects of TaON-Ag in osteogenesis. Results: The TaON-Ag-coated Ti exhibited an effective antibacterial effect against Staphylococcus aureus, coagulase-negative Staphylococcus, and the Gram-negative strains Escherichia coli and Pseudomonas aeruginosa. Using an osteogenic culture with rMSCs and a rat femoral fracture model, the TaON-Ag-coated Ti did not interfere with the ossification of rMSCs in vitro or during fracture healing in vivo. Field-emission scanning electron microscopy (FE-SEM) revealed that coating with TaON-Ag could inhibit pathogen adhesion and biofilm formation in both Staphylococcus aureus and Escherichia coli. Conclusion: Using the proposed novel oxygenation process, TaON-Ag nanocomposite-coated Ti yielded robust biocompatibility and antibacterial ability against common microorganisms in orthopedic infections, thereby demonstrating potential for use in clinical applications.

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Investigation of cellular responses upon interaction with silver nanoparticles

Cited by 9 publications

References 36 publications

Biomechanical Response of Lung Epithelial Cells to Iron Oxide and Titanium Dioxide Nanoparticles

Biomechanical Response of Lung Epithelial Cells to Iron Oxide and Titanium Dioxide Nanoparticles

Bactericidal and Cytotoxic Properties of Silver Nanoparticles

<p>Beneficial Effect of TaON-Ag Nanocomposite Titanium on Antibacterial Capacity in Orthopedic Application</p>

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