Investigation of cat’s-eye zircons from Sri Lanka

Gunawardene, Mahinda; Gunawardene, Marion

doi:10.15506/jog.1988.21.2.88

Cited by 1 publication

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fraction of EC amongst the EE cells remained in the range of 45-51% under these differentiation conditions, which is consistent with their fraction in the body where EC cells make up 50-70% of the EE population in the colon and rectum. 2 To determine whether EC cell enrichment also increased stimulated 5HT secretion, the monolayers were incubated with vehicle (DMSO) or forskolin (10 μM). The 5HT secretion was found to correlate with the observed number of EC cells (Figure 2C).…”

Section: Resultsmentioning

confidence: 99%

“…EE cells consist of at least 15 different subtypes with enterochromaffin (EC) cells being the most abundant, comprising ~40% of EE cells in the gastrointestinal tract. 2,3 EC cells can sense various stimuli from luminal contents (e.g. nutrients, microbial metabolites, irritants, toxins, infection, and mechanical stimuli), and synthesize and secrete the biogenic amine 5hydroxy tryptamine (5HT) also known as serotonin.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enterochromaffin Cell-Enriched Monolayer Platform for Assaying Serotonin Release from Human Primary Intestinal Cells

2020

View full text Add to dashboard Cite

Enteroendocrine (EE) cells within the intestinal epithelium produce a range of hormones that have key roles in modulating satiety and feeding behavior in humans. The regulation of hormone release from EE cells as a potential therapeutic strategy to treat metabolic disorders is highly sought after by the pharmaceutical industry. However, functional studies are limited by the scarcity of EE cells (or surrogates) in both in vivo and in vitro systems. Enterochromaffin (EC) cells are a subtype of EE cell that produce serotonin (5HT). Here, we explored simple strategies to enrich EC cells in in vitro monolayer systems derived from human primary intestinal stem cells. During differentiation of the monolayers, the EC cell lineage was significantly altered by both the culture method (air-liquid interface [ALI] vs. submerged) and the presence of vasoactive intestinal peptide (VIP). Compared with traditional submerged cultures without VIP, VIP-assisted ALI culture significantly boosted the number of EC cells and their 5HT secretion by up to 430% and 390%, respectively. The method also increased the numbers of other subtypes of EE cells such as L cells. Additionally, this method generated monolayers with enhanced barrier integrity so that directional (basal or apical) 5HT secretion was measurable. For all donor tissue, the enriched EC cells improved the signal-to-background ratio and reliability of 5HT release assays. The enhancement in 5HT secretion behavior was consistent over time from a single donor, but significant variation in the amount of secreted 5HT was present among tissues derived from 5 different donors. To demonstrate the utility of the EC-enriched monolayer system, 13 types of pungent food ingredients were screened for their ability to stimulate 5HT secretion. Curcumin found in the spice turmeric derived from the Curcuma longa plant was found to be the most potent secretagogue. This EC-enriched cell-monolayer platform can provide a valuable analytical tool for highthroughput screening of nutrients and gut microbial components that alter the secretion of 5HT.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%