Investigation of Calpain 10 (rs2975760) gene polymorphism in Asian Indians with Gestational Diabetes Mellitus

Abstract

BackgroundType 2 Diabetes Mellitus (T2DM) and Gestational Diabetes Mellitus (GDM) are part of a heterogeneous and complex metabolic group of disorders that share common pathophysiological circumstances, including β-cell dysfunction and insulin resistance. The protein Calpain 10 (CAPN10) plays a role in glucose metabolism, pancreatic β-cell insulin secretion, and thermogenesis.ObjectivePolymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR–RFLP) based genotyping of CAPN10 (rs2975760) polymorphism was carried out in T2DM and GDM with suitable controls for each of the pathologies from the same population. Genomic DNA was isolated from 787 participants, including 250 cases of T2DM, 287 pregnant women, of which 137 were identified as having GDM and the remaining 150 were confirmed as non-GDM, and 250 healthy control volunteers, and association analysis was carried out for genotypes and alleles.ResultsIn the present study, T2DM was compared with healthy controls and was not found to be associated with the CAPN10 C allele (odds ratio, OR: 1.09; 95% CI = 0.8011–1.484; p = 0.5821). GDM also did not show any association when compared with non-GDM (OR: 1.124; 95% CI = 0.7585–1.667; p = 0.5606) respectively.ConclusionOur study suggests that the CAPN10 (rs2975760) polymorphism scrutinized in this study is not associated with T2DM and GDM.

Validation of the association of <i>TCF7L2</i> and <i>SLC30A8</i> gene polymorphisms with post-transplant diabetes mellitus in Asian Indian population

“…However, there is no consensus on molecular diagnosis and screening in identification of diabetes patients. Current diagnosis and screening are based on biochemical analysis of coagulated blood serum (4). The results of the present study reveal that there is an association between TCF7L2 and SLC30A8 gene polymorphisms in PTDM in Asian Indians.…”

“…Both PTDM and type 2 diabetes mellitus (T2DM) are multifactorial metabolic disorders (3). T2DM is a chronic disease, characterized by insulin resistance and impaired insulin secretion from pancreatic β-cells (4). PTDM incidence has been increasing dramatically throughout the globe, in parallel to T2DM (5).…”

Importance of glucokinase -258G/A polymorphism in Asian Indians with post-transplant and type 2 diabetes mellitus

“…The selection of T2DM subjects, controls, and PTDM subjects and the inclusion and exclusion criteria for the specific groups are described in previous publications (1,5,11). We collected 3 mL of serum for biochemical analysis and 2 mL of anticoagulated blood for mutational analysis.…”

“…Impaired β-cell function and insulin resistance are key determinants of T2DM (1). The etiology of T2DM is thought to involve genetic and environmental factors, and the disease is characterized by decreased insulin secretion and insulin resistance (2).…”

Effect of GSTM1, GSTT1, and GSTP1 IIe105Val polymorphisms on susceptiblity to gestational diabetes mellitus

“…However, not all pregnant women exposed to certain risk factors go on to develop gestational diabetes mellitus, suggesting that hereditary factors may contribute to the occurrence of this disease. Currently, many epidemiologic studies have shown that certain genes, such as melatonin receptor type 1B (MTNR1B), lipoprotein lipase gene, potassium channel voltage gated KQT-like subfamily Q, member 1 (KCNQ1), methylenetetrahydrofolate reductase (MTHFR), factor V Leiden (FVL), and adiponectin gene and adenosine deaminase, contribute to the susceptibility to gestational diabetes mellitus (Khan et al, 2014;Ao et al, 2015;Li et al, 2015a;Takhshid et al, 2015a,b).…”