Investigation of Biomolecule Interactions: Optical-, Electrochemical-, and Acoustic-Based Biosensors

Plikusienė, Ieva; Ramanavičienė, Almira

doi:10.3390/bios13020292

Cited by 5 publications

(6 citation statements)

References 34 publications

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“…The simplest detection principle is to directly quantify the electroactive products generated by ALP catalysis through different electrochemical techniques. By taking advantages of the characteristics of ALP substrates and products, different signal amplification strategies, including redox cycling and product-triggered in situ metallization, are introduced into detection systems to amplify the signals [ 29 ].Thus, ALP-based electrochemical immunoassays are widely constructed to determine various targets, including nucleic acids, proteins, bacteria, viruses, biological toxins, andother microorganisms in food matrices [ 30 , 31 ]. In addition, such immunoassays are also developed to determine the pesticides and antibiotics in the environment and agriculture [ 32 , 33 , 34 ].…”

Section: Electrochemical Methodsmentioning

confidence: 99%

Progress in Electrochemical Immunosensors with Alkaline Phosphatase as the Signal Label

Chen,

La,

et al. 2023

Biosensors

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Electrochemical immunosensors have shown great potential in clinical diagnosis, food safety, environmental protection, and other fields. The feasible and innovative combination of enzyme catalysis and other signal-amplified elements has yielded exciting progress in the development of electrochemical immunosensors. Alkaline phosphatase (ALP) is one of the most popularly used enzyme reporters in bioassays. It has been widely utilized to design electrochemical immunosensors owing to its significant advantages (e.g., high catalytic activity, high turnover number, and excellent substrate specificity). In this work, we summarized the achievements of electrochemical immunosensors with ALP as the signal reporter. We mainly focused on detection principles and signal amplification strategies and briefly discussed the challenges regarding how to further improve the performance of ALP-based immunoassays.

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Section: Electrochemical Methodsmentioning

confidence: 99%

Progress in Electrochemical Immunosensors with Alkaline Phosphatase as the Signal Label

Chen,

La,

et al. 2023

Biosensors

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“…The special characteristics of the electrochemical substrates, instrumentation, and techniques endow electrochemical biosensors with intrinsic features that include affordability, low-power requirement, portability, sensitivity, specificity, timeliness, ease of use, feasibility for analyzing turbid, opaque, and colored solutions, and easy integration with PON technologies. These features make electrochemical biosensors ideal to be employed by anyone in remote or resource-limited settings. ,, Currently, electrochemical substrates are manufactured cheaply and on a large scale, while electrochemical instruments are commercially available in sizes so small that we could never imagine but keeping a performance equivalent to traditional instruments with the size of a microwave oven …”

Section: Devices With Unique Intrinsic And/or Acquired Attributesmentioning

confidence: 99%

“…These features make electrochemical biosensors ideal to be employed by anyone in remote or resource-limited settings. 1,5,6 Currently, electrochemical substrates are manufactured cheaply and on a large scale, while electrochemical instruments are commercially available in sizes so small that we could never imagine but keeping a performance equivalent to traditional instruments with the size of a microwave oven. 1 Electrochemical biosensors have rapidly profited from the innovations that other technologies have undergone to empower themselves with unique acquired attributes and capabilities.…”

mentioning

confidence: 99%

Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead

Campuzano

Pingarrón

2023

ACS Sens.

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Electrochemical affinity biosensors are evolving at breakneck speed, strengthening and colonizing more and more niches and drawing unimaginable roadmaps that increasingly make them protagonists of our daily lives. They achieve this by combining their intrinsic attributes with those acquired by leveraging the significant advances that occurred in (nano)materials technology, bio(nano)materials and nature-inspired receptors, gene editing and amplification technologies, and signal detection and processing techniques. The aim of this Perspective is to provide, with the support of recent representative and illustrative literature, an updated and critical view of the repertoire of opportunities, innovations, and applications offered by electrochemical affinity biosensors fueled by the key alliances indicated. In addition, the imminent challenges that these biodevices must face and the new directions in which they are envisioned as key players are discussed.

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“…Acoustic sensors are devices composed of piezoelectric materials, combining mechanical waves and electrical signals to provide detection [ 1 ]. Thanks to their versatility and sensitivity, this class of sensors has become increasingly important in various fields and, in particular, in biomedical applications [ 2 , 3 ]. Bio-related applications of acoustic sensors mostly concern the analysis of various biomolecules and cells, but they have shown great potential also in the early detection of diseases and drug screening.…”

Section: Introductionmentioning

confidence: 99%

A Fast and Reliable Method Based on QCM-D Instrumentation for the Screening of Nanoparticle/Blood Protein Interactions

2023

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The interactions that nanoparticles have with blood proteins are crucial for their fate in vivo. Such interactions result in the formation of the protein corona around the nanoparticles, and studying them aids in nanoparticle optimization. Quartz crystal microbalance with dissipation monitoring (QCM-D) can be used for this study. The present work proposes a QCM-D method to study the interactions on polymeric nanoparticles with three different human blood proteins (albumin, fibrinogen and γ-globulin) by monitoring the frequency shifts of sensors immobilizing the selected proteins. Bare PEGylated and surfactant-coated poly-(D,L-lactide-co-glycolide) nanoparticles are tested. The QCM-D data are validated with DLS and UV-Vis experiments in which changes in the size and optical density of nanoparticle/protein blends are monitored. We find that the bare nanoparticles have a high affinity towards fibrinogen and γ-globulin, with measured frequency shifts around −210 Hz and −50 Hz, respectively. PEGylation greatly reduces these interactions (frequency shifts around −5 Hz and −10 Hz for fibrinogen and γ-globulin, respectively), while the surfactant appears to increase them (around −240 Hz and −100 Hz and −30 Hz for albumin). The QCM-D data are confirmed by the increase in the nanoparticle size over time (up to 3300% in surfactant-coated nanoparticles), measured by DLS in protein-incubated samples, and by the trends of the optical densities, measured by UV-Vis. The results indicate that the proposed approach is valid for studying the interactions between nanoparticles and blood proteins, and the study paves the way for a more comprehensive analysis of the whole protein corona.

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Investigation of Biomolecule Interactions: Optical-, Electrochemical-, and Acoustic-Based Biosensors

Cited by 5 publications

References 34 publications

Progress in Electrochemical Immunosensors with Alkaline Phosphatase as the Signal Label

Progress in Electrochemical Immunosensors with Alkaline Phosphatase as the Signal Label

Electrochemical Affinity Biosensors: Pervasive Devices with Exciting Alliances and Horizons Ahead

A Fast and Reliable Method Based on QCM-D Instrumentation for the Screening of Nanoparticle/Blood Protein Interactions

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