Investigation of binding behavior of important metal ions to thioredoxin reductase using mobility-shift affinity capillary electrophoresis: A preliminary insight into the development of new metal-based anticancer drugs

Alhazmi, Hassan A.; Javed, Sadique A.; Ahsan, Waquar; Rehman, Ziaur; Bratty, Mohammed Al; Deeb, Sami El; Saleh, Safaa F.

doi:10.1016/j.microc.2018.10.035

Cited by 16 publications

(13 citation statements)

References 35 publications

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“…ACE includes several modes based either on (i) the separation of the interacting species and the determination of their equilibrium concentration, such as in the Hummel-Dreyer method [121,124], the vacancy peak method [121,125], frontal analysis [126][127][128], continuous frontal analysis [121,129], and kinetic CE [130], or (ii) the detection of a specific physicochemical property of the complexed analyte or its binding partner, mostly the changes of analyte effective mobility in the mobility shift ACE [131] or changes of migration times of analyte in the partial-filling ACE (PF-ACE) [132,133]. All these methods utilize the differences in the migration velocities of the interacting species; their critical comparison can be found in the recent review [121].…”

Section: Affinity Electrophoresismentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Kašička

2021

Electrophoresis

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The review provides a comprehensive overview of developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) for analysis, microscale isolation, and physicochemical characterization of peptides from 2019 up to approximately the middle of 2021. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis, such as sample preparation, sorption suppression, EOF control, and detection, are presented. New developments in the individual CE and CEC methods are demonstrated and several types of their applications are shown. They include qualitative and quantitative analysis, determination in complex biomatrices, monitoring of chemical and enzymatic reactions and physicochemical changes, amino acid, sequence, and chiral analyses, and peptide mapping of proteins. In addition, micropreparative separations and determination of significant physicochemical parameters of peptides by CE and CEC methods are described.

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Section: Affinity Electrophoresismentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Kašička

2021

Electrophoresis

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“…However, with affinity chromatography, cost considerations become important owing to the use of affinity columns besides a large amount of sample for analysis [13,14]. Recently, ACE was successfully used for studying the interaction of various proteins with different metal ions [15][16][17][18]. Various isoforms of proteins were also investigated simultaneously using ACE, which…”

Section: Analytical Techniques For Protein-metal Interaction Studymentioning

confidence: 99%

“…Dynamic equilibrium mode using mobility shift ACE was selected for this study because it is highly sensitive and is capable of detecting even very weak interactions correctly. e precision of the method can further be increased by calculating the mobility ratio for the interaction screening [15][16][17][18][19]. In ACE, the mobility ratio (R), which is the ratio of the analyte mobility (μ) and can be expressed as…”

Section: Affinity Capillary Electrophoresis (Ace)mentioning

confidence: 99%

Rapid Screening and Estimation of Binding Constants for Interactions of Fe3+ with Two Metalloproteins, Apotransferrin and Transferrin, Using Affinity Mode of Capillary Electrophoresis

Bratty

Alhazmi

Javed

et al. 2021

Journal of Spectroscopy

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The interaction behavior of Fe3+ with transferrin and apotransferrin (iron-free form) was investigated in this study using affinity capillary electrophoresis. Change in the mass and charge of protein upon binding to the metal ion in the capillary tube led to variation in its migration time and was used to measure the noncovalent binding interactions by fast screening method. Acetanilide was used as the electroosmotic flow (EOF) marker to avoid possible errors due to the change in EOF during the experiment. The binding results were calculated from the mobility ratios of protein (Ri) and EOF marker (Rf) using the formula (Ri − Rf)/Rf or ∆R/Rf. For more comprehensive understanding, the kinetics of the interaction was studied and binding constants were calculated. Results showed that the Fe3+ displayed insignificant interaction with both proteins at lower metal ion concentrations (5–25 μmol/mL). However, transferrin exhibited significant interactions with the metal ion at 50 and 100 μmol/mL (ΔR/Rf = 0.0114 and 0.0201, resp.) concentrations and apotransferrin showed strong binding interactions (ΔR/Rf = −0.0254 and 0.0205, resp.) at relatively higher Fe3+ concentrations of 100 and 250 μmol/mL. The binding constants of 18.968 mmol−1 and −13.603 mmol−1 were recorded for Fe3+ interaction with transferrin and apotransferrin, respectively, showing significant interactions. Different binding patterns of Fe3+ with both proteins might be attributed to the fact that the iron-binding sites in transferrin have already been occupied, which was not the case in apotransferrin. The present study may be used as a reference for the investigation of protein-metal ion, drug-protein, drug-metal ion, and enzyme-metal ion interactions and may be helpful to provide preliminary insight into the new metal-based drug development.

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“…Such metal complexes have attained special attention owing to their sensitivity, synthetic flexibility in addition to selectivity towards various metal ions [2]. Transition metal complexes were proved as more advantageous and applicable in comparison with conventional organic-based drugs because the pathogens have developed resistance against these drugs [3]. Likewise, these complexes have also been extensively investigated as bioactive compounds because of their wide-ranging biological activities [4].…”

Section: Introductionmentioning

confidence: 99%

Metal incorporated aminothiazole-derived compounds: synthesis, density function theory analysis,in vitroantibacterial and antioxidant evaluation

et al. 2021

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The present study advocates the combined experimental and computational study of metal-based aminothiazole-derived Schiff base ligands. The structure and electronic properties of ligands have been experimentally studied by spectroscopic methods (UV-Vis, FT-IR, 1 H-NMR and 13 C-NMR), mass spectrometry, elemental analysis and theoretically by density function theory (DFT). Computational calculations employing the B3LYP/6-31 + G(d,p) functional of DFT were executed to explore the optimized geometrical structures of ligands along with geometric parameters, molecular electrostatic potential (MEP) surfaces and frontier molecular orbital (FMO) energies. Global reactivity parameters estimated from FMO energy gaps signified the bioactive nature of ligands. The synthesized ligands were used for chelation with 3 d -transition metals [VO(IV), Cr(III), Fe(II), Co(II), Ni(II), Cu(II) and Zn(II)] in 1 : 2 (metal : ligand) molar ratio. The spectral and magnetic results confirmed the formation of octahedral geometry around all the divalent and trivalent metal centres, whereas the tetravalent vanadyl centres were confirmed to have square-pyramidal geometry. All the as-synthesized compounds were investigated for in vitro antibacterial potential against two Gram-negative ( Salmonella typhimurium and Escherichia coli ) and two Gram-positive ( Bacillus subtilis and Staphylococcus aureus ) bacteria. Antibacterial assay results displayed pronounced activity, and their activity is comparable to that of a standard drug (streptomycin). The antioxidant potential of these compounds was assessed by employing diphenyl picryl hydrazide radical scavenging activity. The results displayed that all the metal chelates have exhibited more bioactivity in contrast with free ligands. The chelation was the main reason for their enhanced bioactivity. These results indicated that the thiazole metal-based compounds could be exploited as antioxidant and antimicrobial candidates.

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Investigation of binding behavior of important metal ions to thioredoxin reductase using mobility-shift affinity capillary electrophoresis: A preliminary insight into the development of new metal-based anticancer drugs

Cited by 16 publications

References 35 publications

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Recent developments in capillary and microchip electroseparations of peptides (2019–mid 2021)

Rapid Screening and Estimation of Binding Constants for Interactions of Fe3+ with Two Metalloproteins, Apotransferrin and Transferrin, Using Affinity Mode of Capillary Electrophoresis

Metal incorporated aminothiazole-derived compounds: synthesis, density function theory analysis,in vitroantibacterial and antioxidant evaluation

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