Investigation of bacteria with polyketide synthase genes and antimicrobial activity isolated from South China Sea sponges

Zhang, W.; Zhang, F.; Li, Z.; Miao, Xiangshui; Meng, Qingqing; Zhang, X.

doi:10.1111/j.1365-2672.2009.04241.x

Cited by 34 publications

(25 citation statements)

References 61 publications

(132 reference statements)

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“…Degenerate forward primer ‘RTRGAYCCNCAGCAICG’ and reverse primer ‘VGTNCCNGTGCCRTG’ (Zhang et al . ; Chakraborty et al . ) were used to analyse the bacterial strain for the ability to excrete biosynthetic metabolites.…”

Section: Methodsmentioning

confidence: 99%

Antibacterial aryl-crowned polyketide from Bacillus subtilis associated with seaweed Anthophycus longifolius

2017

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Aims: Microbiological, biotechnological and chemical characterization of a previously undescribed aryl-crowned polyketide from Bacillus subtilis MTCC 10403 isolated from brown seaweed Anthophycus longifolius with activity against opportunistic Gram-negative food-borne pathogenic bacterial strains. Methods and Results: A culture-dependent method was used to isolate heterotrophic B. subtilis associated with A. longifolius and assessed for its antimicrobial properties. Minimum inhibitory concentration (MIC) of the title compound against the test pathogens was analysed by microtube dilution coupled with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromidebased colorimetric endpoint detection. Bacillus subtilis MTCC 10403 was found to be antagonistic against Gram-negative food-borne pathogenic Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae, Salmonella enterica serotype Typhi, Aeromonas hydrophila and Vibrio sp. (diameter of zone of growth inhibition 13-22 mm). Bacillus subtilis was assessed for the presence of secondary metabolite coding polyketide synthase (pks) gene (KC589397, 700-bp gene product) and carboxylate siderophore framework in the aryl-crowned polyketide designated as 7-O-6 0 -(2″-acetylphenyl)-5 0 -hydroxyhexanoate-macrolactin by exhaustive spectroscopic techniques. The MIC assay showed that the reference antibiotics tetracycline and ampicillin were active at 25 lg ml À1 against the test pathogens, whereas the newly isolated polyketide displayed anti-infective properties against E. coli, A. hydrophilla, P. aeruginosa and Vibrio sp. at a lower concentration (MIC <13 lg ml À1 ). The MIC of the aryl macrolactin against K.pneumoniae was comparable with that of the referral antibiotics (~25 lg ml À1 ).The mode of antimicrobial action of acryl-crowned macrolactin was found to be iron chelating similar to siderophores. Putative biosynthetic pathway of the pks gene product further validated its molecular attributions.Conclusions: This study recognized new variant of antimicrobial aryl-crowned polyketide bearing methyl 6 0 -(2″-acetylphenyl)-5 0 -hydroxyhexanoate moiety at the C-7 position of the macrolactin system from A. longifolius-associated bacterium B. subtilis. Significance and Impact of the Study: This study revealed seaweed-associated micro-organisms as promising biological strata to produce new-generation anti-infective agents.

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Section: Methodsmentioning

confidence: 99%

Antibacterial aryl-crowned polyketide from Bacillus subtilis associated with seaweed Anthophycus longifolius

2017

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“…Marine invertebrates which are abundant in the Indo-Pacific regions, are rich in secondary metabolites and are becoming targets of continuing search for bioactive compounds [189]. The China Sea has become an important source of marine natural compounds since 2001 [190]. Among metazoans, the phylum Porifera contains the taxa which produce the highest diversity of secondary metabolites [191].…”

Section: Ecological and Cultural Aspects Of Sponge Symbiontsmentioning

confidence: 99%

“…The study of metabolite-related genes of microorganisms associated with sponges may give insight into the origin of sponge-derived natural products. Polyketides, comprising a large and structural diverse family of bioactive natural products are one of the most important classes of marine natural compounds [190]. Polyketide synthase genes of host sponge and associated bacteria are predicted to be biosynthetic modules of polyketide analogues as well as phospholipases [51].…”

Section: Ecological and Cultural Aspects Of Sponge Symbiontsmentioning

confidence: 99%

“…The PKS gene-based molecular approach can be applied to efficient screening of strains of pharmaceutical value and prediction of related compounds. This strategy has been employed to discover the efficiency of polyketide production in Firmicutes especially Bacillus , Actinobacteria and Proteobacteria isolated from sponges of the South China Sea [190]. Isolation and culture of symbiotic microorganisms as producers of secondary metabolites as well as transfer of symbiont biosynthetic genes into cultivable bacteria are subjects of ongoing research [149,204].…”

Section: Ecological and Cultural Aspects Of Sponge Symbiontsmentioning

confidence: 99%

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Marine Drugs from Sponge-Microbe Association—A Review

2010

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The subject of this review is the biodiversity of marine sponges and associated microbes which have been reported to produce therapeutically important compounds, along with the contextual information on their geographic distribution. Class Demospongiae and the orders Halichondrida, Poecilosclerida and Dictyoceratida are the richest sources of these compounds. Among the microbial associates, members of the bacterial phylum Actinobacteria and fungal division Ascomycota have been identified to be the dominant producers of therapeutics. Though the number of bacterial associates outnumber the fungal associates, the documented potential of fungi to produce clinically active compounds is currently more important than that of bacteria. Interestingly, production of a few identical compounds by entirely different host-microbial associations has been detected in both terrestrial and marine environments. In the Demospongiae, microbial association is highly specific and so to the production of compounds. Besides, persistent production of bioactive compounds has also been encountered in highly specific host-symbiont associations. Though spatial and temporal variations are known to have a marked effect on the quality and quantity of bioactive compounds, only a few studies have covered these dimensions. The need to augment production of these compounds through tissue culture and mariculture has also been stressed. The reviewed database of these compounds is available at www.niobioinformatics.in/drug.php.

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“…Bacillus and Streptomyces strains have been widely found in seawater, sediment, marine plants and marine animals. The secondary metabolites produced by these species have antibacterial, antifungal and antitumour activities (Zhang et al ). The PKS‐I gene is found in 30·0% of bacteria in the phylum Firmicutes and is widely distributed in Bacillus (Wang et al ).…”

Section: Discussionmentioning

confidence: 99%

Biosynthetic potential of culturable bacteria associated with Apostichopus japonicus

Chen

et al. 2019

J Appl Microbiol

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Aims Isolating culturable bacteria associated with sea cucumber (Apostichopus japonicus) and investigating their potential bioactivities are important approaches to discover natural marine products. Methods and Results A total of 161 isolates were obtained from sea cucumber collected along the Weihai coast of the North Yellow Sea, China. Identification and phylogenetic analysis of 61 isolates were conducted by 16S rRNA gene sequencing. The isolates belonged to 13 genera from 10 families in three phyla, including Firmicutes, Actinobacteria and Proteobacteria. The antimicrobial activities of all strains were determined using six indicator strains. Of the 161 isolates, 93 showed antibacterial activities against at least one of the indicator strains. The 26 strains with the strongest inhibitory effects were selected for screening the biosynthetic gene clusters of polyketide synthase (PKS‐I, ‐II) and nonribosomal peptide synthetase (NRPS). Phylogenetic trees based on the amino acid sequences of the PKS or NRPS genes were constructed. Eleven strains with PKS genes and four strains with NRPS genes were detected. Conclusions The data reveal the diversity of culturable bacteria associated with A. japonicus. Most strains showed broad‐spectrum antimicrobial activities, and some strains with antimicrobial activities possessed PKS and NRPS genes. Significance and Impact of the Study The results suggest that culturable bacteria associated with A. japonicus may act as a promising source of bioactive substances.

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Investigation of bacteria with polyketide synthase genes and antimicrobial activity isolated from South China Sea sponges

Cited by 34 publications

References 61 publications

Antibacterial aryl-crowned polyketide from Bacillus subtilis associated with seaweed Anthophycus longifolius

Antibacterial aryl-crowned polyketide from Bacillus subtilis associated with seaweed Anthophycus longifolius

Marine Drugs from Sponge-Microbe Association—A Review

Biosynthetic potential of culturable bacteria associated with Apostichopus japonicus

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