Investigation of architecture development and phosphate distribution in <i>Chlorella</i> biofilm by complementary microscopy techniques

Osorio, Jairo Hernán Moreno; Benettoni, Pietro; Schmidt, Matthias; Stryhanyuk, Hryhoriy; Schmitt‐Jansen, Mechthild; Pinto, Gabriele; Pollio, Antonino; Frunzo, Luigi; Lens, Piet N.L.; Richnow, Hans H.; Esposito, Giovanni; Musat, Niculina

doi:10.1093/femsec/fiz029

Cited by 11 publications

(10 citation statements)

References 43 publications

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“…Delayed extraction is now widely used to image organic samples and lately was shown to achieve a 108 nm lateral resolution to visualize single particle in algal biofilms [43,44]. ToF-SIMS is a useful method for studying small molecules [45][46][47] and lipids in cells, especially in lipid-related diseases, such as cancer, Duchenne muscular dystrophy and atherosclerosis ( Figure 2) [48,49]. absorbed by an atom: a photo-electron is ejected from a core orbital of the excited atom leaving a vacancy which is then filled by another electron from a higher orbital.…”

Section: Secondary Ion Mass Spectrometry (Sims)mentioning

confidence: 99%

Subcellular Chemical Imaging: New Avenues in Cell Biology

Decelle

Veronesi

Gallet

et al. 2020

Trends in Cell Biology

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To better understand the physiology and acclimation capability of a cell, one of the great challenges of the future is to access the interior of a cell and unveil its chemical landscape (composition and distribution of elements and molecules). Chemical imaging has greatly improved in sensitivity and spatial resolution to visualize and quantify nutrients, metabolites, toxic elements, and drugs in single cells at the subcellular level. This review aims at presenting the current potential of these emerging imaging technologies and guiding biologists towards a strategy for interrogating biological processes at the nanoscale. We also describe different solutions to combine multiple imaging techniques in a correlative way and provide perspectives and future directions for integrative subcellular imaging across different disciplines.

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Section: Secondary Ion Mass Spectrometry (Sims)mentioning

confidence: 99%

Subcellular Chemical Imaging: New Avenues in Cell Biology

Decelle

Veronesi

Gallet

et al. 2020

Trends in Cell Biology

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“…HIM-SIMS also appears to be a promising tool to study intracellular deposits of certain chemical elements. An example are phosphate granules in algal biofilms, which were previously investigated using multiple microscopes in a correlative study [46]. Another, certainly more challenging, example would be the identification of iron-sulfur clusters in microbes [47] or mitochondria [48] by HIM-SIMS.…”

Section: Secondary Ion Mass Spectrometry (Sims)mentioning

confidence: 99%

“…HIM imaging has already been used to study numerous microorganisms including bacteria [6,18,19,60,79,80], bacteriophages [17,18,81], yeast [82], protozoa [83][84][85], and microalgae [46]. The highly resolved and contrast-rich views of the tiniest creatures that were obtained in these studies are discussed in the following.…”

Section: Microbiologymentioning

confidence: 99%

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Bio-imaging with the helium-ion microscope: A review

Schmidt¹,

Byrne²,

Maasilta³

2021

Beilstein J. Nanotechnol.

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Scanning helium-ion microscopy (HIM) is an imaging technique with sub-nanometre resolution and is a powerful tool to resolve some of the tiniest structures in biology. In many aspects, the HIM resembles a field-emission scanning electron microscope (FE-SEM), but the use of helium ions rather than electrons provides several advantages, including higher surface sensitivity, larger depth of field, and a straightforward charge-compensating electron flood gun, which enables imaging of non-conductive samples, rendering HIM a promising high-resolution imaging technique for biological samples. Starting with studies focused on medical research, the last decade has seen some particularly spectacular high-resolution images in studies focused on plants, microbiology, virology, and geomicrobiology. However, HIM is not just an imaging technique. The ability to use the instrument for milling biological objects as small as viruses offers unique opportunities which are not possible with more conventional focused ion beams, such as gallium. Several pioneering technical developments, such as methods to couple secondary ion mass spectrometry (SIMS) or ionoluminescence with the HIM, also offer the possibility for new and exciting research on biological materials. In this review, we present a comprehensive overview of almost all currently published literature which has demonstrated the application of HIM for imaging of biological specimens. We also discuss some technical features of this unique type of instrument and highlight some of the new advances which will likely become more widely used in the years to come.

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“…A commonly employed complementary approach combines electron microscopy (EM) with secondary ion mass spectrometry (SIMS), thus providing high-resolution EM imaging together with elemental, molecular-and isotope-resolved SIMS analysis. [1][2][3] To further increase the resolution and obtain more surface-detailed images, helium ion microscopy (HIM) may also be applied in combination with EM and SIMS. 4 Electron-and ion-probe experiments require an ultrahigh vacuum (UHV) sample environment and demand distinct sample properties (e.g., regarding thickness, roughness, conductivity, etc.…”

Section: Introductionmentioning

confidence: 99%

Surface cleaning and sample carrier for complementary high-resolution imaging techniques

Benettoni

Holbrook

et al. 2020

Biointerphases

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Nowadays, high-resolution imaging techniques are extensively applied in a complementary way to gain insights into complex phenomena. For a truly complementary analytical approach, a common sample carrier is required that is suitable for the different preparation methods necessary for each analytical technique. This sample carrier should be capable of accommodating diverse analytes and maintaining their pristine composition and arrangement during deposition and preparation. In this work, a new type of sample carrier consisting of a silicon wafer with a hydrophilic polymer coating was developed. The robustness of the polymer coating toward solvents was strengthened by cross-linking and stoving. Furthermore, a new method of UV-ozone cleaning was developed that enhances the adhesion of the polymer coating to the wafer and ensures reproducible surface-properties of the resulting sample carrier. The hydrophilicity of the sample carrier was recovered applying the new method of UV-ozone cleaning, while avoiding UV-induced damages to the polymer. Noncontact 3D optical profilometry and contact angle measurements were used to monitor the hydrophilicity of the coating. The hydrophilicity of the polymer coating ensures its spongelike behavior so that upon the deposition of an analyte suspension, the solvent and solutes are separated from the analyte by absorption into the polymer. This feature is essential to limit the coffee-ring effect and preserve the native identity of an analyte upon deposition. The suitability of the sample carrier for various sample types was tested using nanoparticles from suspension, bacterial cells, and tissue sections. To assess the homogeneity of the analyte distribution and preservation of sample integrity, optical and scanning electron microscopy, helium ion microscopy, laser ablation inductively coupled plasma mass spectrometry, and time-of-flight secondary ion mass spectrometry were used. This demonstrates the broad applicability of the newly developed sample carrier and its value for complementary imaging.

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Investigation of architecture development and phosphate distribution in Chlorella biofilm by complementary microscopy techniques

Cited by 11 publications

References 43 publications

Subcellular Chemical Imaging: New Avenues in Cell Biology

Subcellular Chemical Imaging: New Avenues in Cell Biology

Bio-imaging with the helium-ion microscope: A review

Surface cleaning and sample carrier for complementary high-resolution imaging techniques

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