Investigation of apoptosis based on fluorescence lifetime imaging microscopy with a mitochondria-targeted viscosity probe

Abstract

Here we designed a mitochondria-targeted two-photon viscosity probe (TPA-Mit), which sensitively responds to viscosity variations with fluorescence lifetime changes.

Lifetime‐Based Responsive Probes: Design and Applications in Biological Analysis

“…It is demonstrated that the lifetime of Biotin‐V can be used to monitor viscosity in mitochondrial. In addition, Liu's group designed another viscosity probe based on two‐photon (TPA‐Mit), as shown in Figure 6b [44] . Using the TPA‐Mit probe, it could be detected that after SKOV‐3 cells were induced to apoptosis by paclitaxel (PTX), the fluorescence lifetime of TPA‐Mit was increased.…”

“…(b) Chemical structures of TPA‐Mit and FLIM images of cells incubated with PTX at different times Scale bar: 10 μm. Reproduced with permission [44] . Copyright 2021, The Royal Society of Chemistry.…”

“…In addition, Liu's group designed another viscosity probe based on two-photon (TPA-Mit), as shown in Figure 6b. [44] Using the TPA-Mit probe, it could be detected that after SKOV-3 cells were induced to apoptosis by paclitaxel (PTX), the fluorescence lifetime of TPA-Mit was increased. This proves that the degree of apoptosis is positively correlated with its viscosity.…”

“…Reproduced with permission. [44] Copyright 2021, The Royal Society of Chemistry. (c) Structure of MMP probe.…”

Fine Tuning of Multiphoton AIE Emission Behavior, Organelle Targeting, and Fluorescence Lifetime Imaging of Terpyridine Derivatives by Alkyl Chain Engineering

“…Multiphoton fluorescence lifetime imaging microscopy (FLIM) is considered to be an excellent alternative to traditional fluorescence imaging, which could effectively eliminate short-lived background fluorescence and provide fluorescence images with a high signal-to-noise ratio. − So far, multiphoton FLIM imaging agents are mostly organometallic complexes containing heavy metals such as europium, ruthenium, and iridium . These complexes with long excited-state lifetimes are expensive and even potentially toxic, which severely limits their biological applications.…”