Investigation of antioxidant and anticancer potential of fucoidan from Sargassum polycystum

Palanisamy, Subramanian; Vinosha, Manoharan; Manikandakrishnan, Muthushanmugam; Anjali, Ravichandran; Rajasekar, Periyannan; Marudhupandi, Thangapandi; Manikandan, R.; Vaseeharan, Baskaralingam; Prabhu, Nagesh

doi:10.1016/j.ijbiomac.2018.04.163

Cited by 88 publications

(50 citation statements)

References 64 publications

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“…When cells were treated with low concentration of WLP (1 mg/mL), the basic morphology of HepG2 cancer cells altered unobviously. However, HepG2 cells cultured with medium (5 mg/mL) and high (9 mg/mL) doses of WLP showed obvious characteristics of apoptotic, including cell contraction deformation, blurred contour, chromatin condensation and nuclear condensation (Palanisamy et al, 2018). In this study, the apoptotic morphology of HepG2 cells induced by WLP were ulteriorly verified by DAPI-staining.…”

Section: Wlp Induces Apoptosis Of Hepg2 Cellsmentioning

confidence: 59%

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Inhibition Effect of Walnut Leaf Polysaccharide on HepG2 Cells in vitro

Yang¹,

Xiao²,

Yu³

et al. 2019

American Journal of Biochemistry and Biotechnology

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In this study, the effect of Walnut Leaf Polysaccharides (WLP) on human liver tumor HepG2 cells was investigated. The results showed that WLP had dose-dependent and time-dependent inhibitory effects on HepG2 cell viability. Typical apoptosis characteristics were observed in WLP-treated HepG2 cells through 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolyl-carbocyanine iodide (DAPI) staining and Acridine Orange (AO)/Ethidium Bromide (EB) dual staining. Meanwhile, significant decrease in Mitochondrial Membrane Potential (MMP) and increase in intracellular Reactive Oxygen Species (ROS) levels of WLP-induced HepG2 cells were detected by 5,5′,6,6′-Tetrachloro-1,1′,3,3′tetraethylbenzimidazolcarbocyanine iodide (JC-1) and 2′,7′-Dichlorodihydrofluorescein Diacetate (DCFH-DA) fluorescent probes, respectively. Moreover, cell viability, MMP and ROS level of HepG2 cells were observed after intervention with antioxidant NAC. Thus, we speculated that WLP may induce apoptosis of HepG2 cells by decreasing MMP and increasing intracellular ROS levels. The results suggest that WLP could be developed as a promising natural anticancer active substance in the pharmaceutical and functional food industries.

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Section: Wlp Induces Apoptosis Of Hepg2 Cellsmentioning

confidence: 59%

“…The cellular morphology changes of cells were visualized by DAPI staining and AO/EB dual staining methods (Palanisamy et al, 2018). HepG2 cells in logarithmic growth-phase were seeded in 6-well plates (3×10 4 /well).…”

Section: Dapi and Ao/eb Staining Analysismentioning

confidence: 99%

Inhibition Effect of Walnut Leaf Polysaccharide on HepG2 Cells in vitro

Yang¹,

Xiao²,

Yu³

et al. 2019

American Journal of Biochemistry and Biotechnology

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“…Therefore, the pH value of the applied solvents is critical during chromatographic purification. One option for this uses anionic exchange resins (e.g., diethylaminoethyl cellulose or DEAE-cellulose), which was performed at pH 7.2 using 0.1 M sodium phosphate buffer [140]. An alternative is cationic dyes (e.g., toluidine blue-or perylene diimide derivative), modified resins or chitosan functioning in buffered solutions [27,102].…”

Section: Purificationmentioning

confidence: 99%

“…Both anion exchange and dye affinity chromatography involve the use of highly concentrated NaCl elution solvents. As a result, a subsequent purification step using chromatographic gel permeation [141] or dialysis [140] is required to remove salts, increasing the production costs. Other methods based on the use of biological macromolecules, such as lectins and anti-thrombin III, were also reported [53].…”

Section: Purificationmentioning

confidence: 99%

Fucoidans: Downstream Processes and Recent Applications

Zayed

Ulber

2020

Marine Drugs

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Fucoidans are multifunctional marine macromolecules that are subjected to numerous and various downstream processes during their production. These processes were considered the most important abiotic factors affecting fucoidan chemical skeletons, quality, physicochemical properties, biological properties and industrial applications. Since a universal protocol for fucoidans production has not been established yet, all the currently used processes were presented and justified. The current article complements our previous articles in the fucoidans field, provides an updated overview regarding the different downstream processes, including pre-treatment, extraction, purification and enzymatic modification processes, and shows the recent non-traditional applications of fucoidans in relation to their characters. of 22reproductive, immune and cell-to-cell communicative roles [23]. As recommended by the International Union of Pure and Applied Chemistry (IUPAC), fucoidans is a general term used to describe sulfated L-fucose-based polymers including sulfated fucans cited by the Swedish scholar Kylin, as well as other fucose-rich sulfated heteropolysaccharides [23,28]. Their chemical structures, in terms of monomeric composition and branching, are quite simple in marine invertebrates compared to their analogues in brown algae [13,29].Hundreds of articles have thoroughly discussed and reviewed the biological, pharmacological and pharmaceutical applications of fucoidans [30][31][32][33], including nanomedicine, [34] which has made it a hot topic in the last few decades [35][36][37]. All these studies tried to investigate fucoidans molecular mechanisms in relation to their chemical structure and physicochemical properties. Therefore, different hypotheses were suggested for each activity, such as anti-tumor [31,[38][39][40], anti-coagulant [41,42], anti-viral [43,44] and anti-inflammatory activity [45,46]. These investigations revealed that various factors are relevant, such as molecular weight, sulfation pattern, sulfate content and monomeric composition [47][48][49]. For example, different fractions were produced with different physicochemical properties in our previous experiments; sulfation pattern and sulfate content were highly related to anti-viral and cytotoxic activities against HSV-1 and Caco-2 cell lines, respectively, while molecular weight and sugar composition were potential factors in anti-coagulation activity [41,50]. In addition, degree of purity was reported as an influential factor [32], where co-extracted contaminants (e.g., phlorotannins or polyphenols) could lead to significant interference in anti-oxidant activity and, consequently, cosmetic applications [51,52].Therefore, several key production challenges regarding fucoidans were discussed in our last review article in order to obtain a product that follows the universal good manufactured practice (GMP) guidelines. The article discussed sources of heterogeneity in extracted fucoidans, including the different biotic (e.g., biogenic, geographical and seas...

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“…Fucoidan, sulfated heteropolysaccharide, presents in marine brown seaweed which is a very popular raw material in Vietnam and many parts of the world . This polysaccharide is known to be involved in many biological activities including anticoagulant, anti‐viral, anti‐oxidation, anti‐cancer, anti‐inflammatory, anti‐tumor, and anti‐diabetes . Biological activities of fucoidan are associated with their structural features, especially sulfate group attached to the fucose monomer of fucoidan .…”

Section: Introductionmentioning

confidence: 99%

Ultrasound‐Assisted Extraction of Fucoidan from Vietnamese Brown Seaweed Sargassum mcclurei and Testing Bioactivities of the Extract

Sung

Dat

et al. 2020

ChemistrySelect

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In this study, fucoidan was extracted from the Vietnamese brown seaweed Sargassum mcclurei by ultrasound -assisted extraction method (UAE) using ethanol as a solvent. The effects of single factors on total polysaccharide (TPS) content were then investigated, followed by the screening of main factors affecting TPS content based on their effect sizes and p-values in analysis of variance. The response surface methodology using the Box-Behnken design was applied to investigate simultaneous effects of four main factors including solvent-tomaterial ratio, extraction time, ultrasonic power, and extraction temperature. The optimal conditions for fucoidan extraction were identified with solvent-to-material ratio of 24 : 1 mL/g, extraction time of 49 min, ultrasonic power of 360 W, and extraction temperature of 54 � C. The highest yield of TPS obtained under these conditions was 7.1203 mg/g. Besides, extraction of fucoidan using hot water method was also carried out to compare with UAE method in terms of TPS content. The bioactivity tests showed the extract had the anti-oxidation activity with the half-maximal inhibitory concentration (IC 50 ) value of 0.4261 � 0.0008 mg/mL, cytotoxic effect on breast carcinoma cells and human lung carcinoma with IC 50 values of 95.80 � 4.75 and 129.00 � 0.66 μg/mL, respectively.

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Investigation of antioxidant and anticancer potential of fucoidan from Sargassum polycystum

Cited by 88 publications

References 64 publications

Inhibition Effect of Walnut Leaf Polysaccharide on HepG2 Cells in vitro

Inhibition Effect of Walnut Leaf Polysaccharide on HepG2 Cells in vitro

Fucoidans: Downstream Processes and Recent Applications

Ultrasound‐Assisted Extraction of Fucoidan from Vietnamese Brown Seaweed Sargassum mcclurei and Testing Bioactivities of the Extract

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