Investigation of antioxidant ability of grape seeds extract to prevent oxidatively induced DNA damage by gas chromatography-tandem mass spectrometry

Aybastıer, Önder; Dawbaa, Sam; Demir, Cevdet

doi:10.1016/j.jchromb.2017.11.044

Cited by 30 publications

(16 citation statements)

References 23 publications

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“…Several studies with extracts from winemaking byproducts rich in phenolic compounds have been carried out for evaluation of antioxidant properties [23][24][25]. Although in vitro antioxidant activity of winemaking byproducts has been widely studied by different spectrophotometric methods, studies on the in vitro anti-cholinesterase properties of these products are still scarce.…”

Section: Introductionmentioning

confidence: 99%

Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour

et al. 2020

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Extraction solvent is a very important factor in the recovery of antioxidants from natural matrices. In this study, the effect of three solvents (ethanol, ethanol/water and water) on the phenolic composition, antioxidant and anti-cholinesterase activities and electrochemical behaviour of four winemaking byproducts (seeds, skins, stems, and pomace) was evaluated. Phenolic composition was determined by the Folin–Ciocalteu method and ultra-high-performance liquid chromatography (UHPLC), antioxidant activity by the capacity to scavenge 2,2-diphenyl-1-picrylhydrazyl and hydroxyl radicals, anti-cholinesterase activity by the Ellman’s method, and electrochemical behaviour by cyclic voltammetry. Eight phenolic compounds were quantified with higher content in water/ethanol extracts (e.g., epicatechin in pomace: 17 mg/100 g vs. 7 and 6 mg/100 g in ethanol and water extracts, respectively), although there were some exceptions (e.g., gallic acid in seeds was most abundant in water extracts). Moreover, the highest total phenolic content (TPC) and antioxidant activity were found in ethanol/water extracts (between 2 and 30-fold the values of the other extracts). Overall, the most active extracts in inhibiting both acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes were ethanol/water and ethanol extracts from seeds (between 31.11 and 53.90%). The electrochemical behaviour allowed for differentiating the extracts depending on the solvent and the byproduct. Our findings indicate that winemaking byproducts represent a source of phenolic compounds with antioxidant and anti-cholinesterase activities and suggest that cyclic voltammetry is a promising technique to evaluate the phenolic extraction process from these byproducts.

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Section: Introductionmentioning

confidence: 99%

Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour

et al. 2020

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“…Furthermore, plant extracts generally contain polyphenols and are widely formulated in cosmetics and in functional foods as natural antioxidants. In fact, it is well‐known that grape seeds contain catechin, gallic acid, chlorogenic acid, and ellagic acid as polyphenolic compounds . Furthermore, several polyphenols, such as quercetin, kaempferol, pterostilbene, and cynaropicrin, have been reported as activators of Nrf2 signaling .…”

Section: Discussionmentioning

confidence: 99%

A red pumpkin seed extract reduces melanosome transfer to keratinocytes by activation of Nrf2 signaling

Endo

Mizutani

Okano

et al. 2018

J of Cosmetic Dermatology

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“…The treatment of PD is still a challenging issue to the physician and there is no doubt that there is a need to find new therapeutic agents to improve therapeutic outcome or decrease neuronal loss in the course of the disease. In this study, the effect of a standardized GSE rich in proanthocyanidins that possesses antioxidant [23,24] and antiapoptotic properties [23,25], was therefore evaluated for a potential neuroprotective effect against the rotenone neurotoxicity. Our findings suggest that GSE could be of value in this respect.…”

Section: Rosmentioning

confidence: 99%

“…Grape seed polyphenolic extracts obtained from Vitis vinifera seeds are rich in the polyphenolic compounds proanthocyanidins, (+)catechin, (-)-epicatechin, (-)-epicatechin-O-gallate [21,22]. GSEs exert antioxidant [23,24] and antiapoptotic [23,25] actions. In vitro, GSE protected neonatal mouse hippocampal neurons against glutamate excitotoxicity with the effect being attributable to polyphenols and/or procyanidin oligomers [26].…”

Section: Introductionmentioning

confidence: 99%

Grape Seed Extract Exerts an Anti-Apoptotic Effect and Attenuates the Decrease in Striatal Tyrosine Hydroxylase in Rotenone-Treated Mice

Abdel-Salam

El-Shamarka

Omara

2019

ROS

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The potential neuroprotective effect of grape seed extract (GSE) was evaluated in the rotenoneinduced Parkinson's disease in mice. Rotenone was administered at the dose of 1.5 mg/kg subcutaneously (sc) three times per week for 2 weeks alone or in combination with GSE at doses of 13.5 and 27 mg/kg, sc, daily. The control group received the vehicle. The brain levels of the lipid peroxidation product malondialdehyde (MDA), reduced glutathione (GSH), nitric oxide (nitrite), and paraoxonase-1 (PON-1) were determined. Histopathology, caspase-9 immunohistochemistry in different brain regions, and tyrosine hydroxylase immunoreactivity (TH-ir) in the substantia nigra were performed. Behavioral testing included rearing activity, locomotor activity, and stair test paradigms. Results indicated significantly increased lipid peroxidation and nitric oxide contents along with a significant decrease in GSH level and marked inhibition of PON-1 activity in the striatum and in the rest of the brain tissue in rotenone-treated mice. Rotenone caused significant decreases in rearing and locomotor activities and impaired motor strength. Treatment with GSE at 27 mg/kg resulted in decreased MDA and nitric oxide by 22.8%/17.9% and 38.5%/45.5%, respectively, in the striatum and the rest of the brain. GSH was increased by 20.8% and 26%, while PON-1 activity increased by 204% and 142.9% after GSE treatment in the striatum and in the rest of the brain tissue, respectively, compared with the corresponding rotenone control values. GSE given at 27 mg/kg almost completely corrected the decrease in motor activity and motor strength caused by rotenone. Neuronal degeneration and the increase in caspase-9 expression caused by rotenone in different brain regions as well as the loss of substantia nigra TH-ir were markedly reduced by GSE. These data indicate that GSE was effective in improving brain oxidative stress and in preventing the behavioral deficits and neurodegeneration induced by rotenone in the mouse brain. It is suggested that GSE might be useful as an adjunctive treatment in patients with Parkinson's disease.

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Investigation of antioxidant ability of grape seeds extract to prevent oxidatively induced DNA damage by gas chromatography-tandem mass spectrometry

Cited by 30 publications

References 23 publications

Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour

Extraction of Antioxidants from Winemaking Byproducts: Effect of the Solvent on Phenolic Composition, Antioxidant and Anti-Cholinesterase Activities, and Electrochemical Behaviour

A red pumpkin seed extract reduces melanosome transfer to keratinocytes by activation of Nrf2 signaling

Grape Seed Extract Exerts an Anti-Apoptotic Effect and Attenuates the Decrease in Striatal Tyrosine Hydroxylase in Rotenone-Treated Mice

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