Investigation of antimicrobial activities of some sideritis species

Abstract

This research has been conducted on tea grown in the western Mediterranean region and also on the consumption of Sideritis stricta and Sideritis condensate species, in order to investigate their antimicrobial activity against 6 pathogenic bacteria. The plants were collected and dried at room temperature in the flowering stage. The essential oil of the plants was obtained with a Clevenger apparatus by the hydrodistillation method. Antibacterial activities of the extracts were determined with the disc diffusion method. In this method, MIC of the extracts which showed antibacterial activity were determined with the microdilution method. Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 27853, Salmonella enteretidis RSK 95046, Klebsiella pneumoniae ATCC 700613 were used as test bacteria. The results were evaluated according to the Clinical and Laboratory Standards Institute (CLSI) criteria. Ampicillin and penicillin were used as the positive control antibiotics. It was determined that hot water extracts of both plants did not have antibacterial activity. The results of the statistical analysis showed that there was a significant difference (P<0.05) between the antimicrobial activities of the essential oil of Sideritis stricta and Sideritis condensate. It was found that there was no significant difference between the antimicrobial activity of Sideritis stricta and ampicillin used as a control antibiotic. On the other hand, the antimicrobial activity of Sideritis condensata was lower when compared to the control antibiotic.