Investigation of antibacterial mechanism and identification of bacterial protein targets mediated by antibacterial medicinal plant extracts

Yong, Ann-Li; Ooh, Keng-Fei; Ong, Hean-Chooi; Chai, Tsun-Thai; Wong, Fai-Chu

doi:10.1016/j.foodchem.2014.11.103

Cited by 27 publications

(17 citation statements)

References 27 publications

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“…Medicinal plants have been traditionally used for the treatment of diseases caused by microorganisms, and the extracts and bioactive compounds from medicinal plants target a wide range of infections in holistic health care. Research on plant-derived substances, which confirmed their activities and determined their specific mechanisms of action against pathogenic organisms, has been widely pursued [8,9]. In the current study, the response patterns of bacterial populations to plant extracts showed changes in granularity and membrane permeability.…”

Section: Discussionmentioning

confidence: 60%

“…The granularity of the bacteria was lost and the bacterial membranes were damaged by the tested treatments. Most studies have suggested that plant extracts inhibited the growth of bacteria by damaging the cell wall and the cell membrane, thus increasing membrane permeability and causing death of the bacteria [8][9][10]. By using FACS, we found that increasing the extract dose caused further loss of granularity, and of intracellular functions, subsequently inducing cell membrane injuries, and eventually leading to cell death.…”

Section: Discussionmentioning

confidence: 82%

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Antibacterial potential of extracts of various parts of Catunaregam tomentosa (Blume ex DC) Tirveng and their effects on bacterial granularity and membrane integrity

Thimabut¹,

Keawkumpai²,

Permpoonpattana³

et al. 2018

Trop. J. Pharm Res

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Purpose: To investigate the antibacterial activity of extracts from Catunaregam tomentosa on Bacillus subtilis and Staphylococcus aureus, and the bacterial responses to the extracts.Methods: The antibacterial activity of fruit, leaf and stem bark extracts were evaluated against B. subtilis (ATCC6633) and S. aureus (ATCC25923). Using a disc diffusion method, extracts at concentrations ranging from 50 -1,000 µg/disc were tested. The minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) of the extracts against the test bacteria were determined. Fluorescent activated cell sorting (FACS) was used to assess the responses of both types of bacteria to the extracts. Results: The fruit and leaf extracts at 1,000 µg/disc showed optimum efficacy against B. subtilis and S. aureus with MIC of 1,000 µg/mL against both B. subtilis and S. aureus, for the fruit and the leaf extracts. With increasing doses of fruit and leaf extracts at 6 h of incubation, FACS profiles revealed that cell death for B. subtilis increased. The fruit and leaf extracts of C. tomentosa also exhibited antibacterial activity against S. aureus in a dose-and time-dependent manner. The bacteria initially lost their granularity, then lost membrane integrity, and consequently died. Conclusion: The fruit and leaf extracts of C. tomentosa exhibit significant antibacterial potential against Gram-positive bacteria by damaging bacterial granularity and membrane integrity. This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest

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Section: Discussionmentioning

confidence: 60%

Section: Discussionmentioning

confidence: 82%

Antibacterial potential of extracts of various parts of Catunaregam tomentosa (Blume ex DC) Tirveng and their effects on bacterial granularity and membrane integrity

Thimabut¹,

Keawkumpai²,

Permpoonpattana³

et al. 2018

Trop. J. Pharm Res

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“…Thus, the synergistic effects, and the diversity of major and minor constituents present in the essential oils should be taken into account for their biological activity. The significant antibacterial activity of the aqueous extract of M. piperita against S. typhi and E. coli has been reported, the crude ethanolic extract and its subsequent solvent fractions petroleum ether, dichloromethane and ethyl acetate were proved significant antibacterial against E. coli and K. pneumonia 32 . The EOs of M. piperita have exhibited moderate activity against the hyphae and spores of Aspergillus niger 33 .…”

Section: Discussionmentioning

confidence: 98%

“…Studies are in progress in open fields to evaluate any effect of these natural substances on tomato plant development and on qualitative and quantitative tomato fruit production. The inhibitory action of the EO could be attributed to hydrophobic nature of EOs that allows them to penetrate microbial cells and cause alterations in its structure and functionality as indicated by the study of Yong et al 32 .…”

Section: Discussionmentioning

confidence: 99%

Chemical Composition and in vivo Efficacy of the Essential Oil of Mentha piperita L. in the Suppression of Crown Gall Disease on Tomato Plants

et al. 2019

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This study was undertaken to determine the antibacterial efficacy of the essential oil (EO) of peppermint (Mentha piperita L.), in vitro and in vivo, against the phytopathogenic bacteria Agrobacterium tumefaciens (A. tumefaciens). The EO composition of M. piperita L. was investigated by Gas chromatography-mass spectrometry (GC/MS) with 26 identified volatile constituents. The major constituents were menthol (33.59%) and iso-menthone (33.00%). This EO exerted a bactericidal activity against multiple strains of Agrobacterium species with minimum inhibitory concentration (MIC) values ranged from 0.01 to 12.50 mg/mL. In planta experiments, M. piperita EO, tested at concentration of 200 mg/ mL, completely inhibited the formation of tumors on tomato plants inoculated with pathogenic strain A. tumefaciens ATCC 23308 T. These results suggest that M. piperita EO could be used to control plant bacterial disease caused by A. tumefaciens.

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“…Yong et al have identified several distinct proteins with up-regulated expression following medicinal plant exposure, namely chaperonin (60 kDa), flagellin, triacylglycerol lipase, outer membrane protein A, N-acetylmuramoyl-L-alanine amidase, 30S ribosomal protein s1, and stringent starvation protein A. The paper suggests common antibacterial routes for different natural antimicrobial treatments [57].…”

Section: Alterations In Regulation Of Gene Expressionmentioning

confidence: 99%

Nano-Antimicrobial Solutions Using Synthetic-Natural Hybrid Designs

Mocan¹,

Moşteanu²,

Matea³

et al. 2019

Nanomedicines

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Nanotechnology potential in antimicrobial therapy is increasingly demonstrated by various data. Results reveal antibacterial properties, comparable to that of conventional antibiotics. Working on parallel experiments, researchers continue to bring evidence demonstrating age-old-recognized antibacterial properties of various natural components of plant and animal origin. Later years brought an increasing trend for combining synthetic and natural composition in new constructs. The tendency aims to bring more on different essential aspects, such as active substance release, improvement of antibacterial effect, and up-regulation of the mechanisms at the structure-cell interface. Present chapter structures the up-to-date achievements in the field, including the concept of design, biological effects, benefits, mechanisms, and limitations of the field. Also, expected future research directions are to be discussed.

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Investigation of antibacterial mechanism and identification of bacterial protein targets mediated by antibacterial medicinal plant extracts

Cited by 27 publications

References 27 publications

Antibacterial potential of extracts of various parts of <i>Catunaregam tomentosa</i> (Blume ex DC) Tirveng and their effects on bacterial granularity and membrane integrity

Antibacterial potential of extracts of various parts of <i>Catunaregam tomentosa</i> (Blume ex DC) Tirveng and their effects on bacterial granularity and membrane integrity

Chemical Composition and <i>in vivo</i> Efficacy of the Essential Oil of <i>Mentha piperita</i> L. in the Suppression of Crown Gall Disease on Tomato Plants

Nano-Antimicrobial Solutions Using Synthetic-Natural Hybrid Designs

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