Investigation of anti-inflammatory and anti-arthritic potentials of Terminalia catappa bark using in vitro assays and carrageenan-induced inflammation, complete Freund's adjuvant induced arthritis model in rats

Daram, Prasanthi; Jitta, Srinivas Reddy; Shreedhara, CS; Misra, Chandra Sekhar; Gourishetti, Karthik; Lobo, Richard

doi:10.1016/j.sajb.2021.05.010

Cited by 11 publications

(3 citation statements)

References 24 publications

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“…Crude extracts from T. catappa bark, which Aboriginal people use to treat a sore throat, were anti-inflammatory. When Daram et al (2021) compared ethanol and water extracts from T. catappa bark, ethanol extract was better in inhibiting 50% of protein denaturation in the in vitro egg-albumin denaturation assay, and diclofenac was used as a positive control [266]. Both extracts reduced carrageenan-induced paw oedema at a 500 mg/kg concentration in the rat.…”

Section: Anti-inflammatory Crude Extractsmentioning

confidence: 99%

“…Betulinic acid reduced levels of COX-2, NO, TNF-α, and IL-1β in tissues obtained from mice with λ-carrageenan-induced paw oedema [294]. Leaves extract reduced TPA-induced ear oedema in both acute and chronic male ICR (Institute of Cancer Research) mice models by inhibiting myeloperoxidase (MPO) activity [242]; bark extract reduced carrageenan-induced paw oedema by inhibiting cellular infiltration and MPO activity [266]; stem bark extract inhibited IL-1β and nitrite production in RAW 264.7 cells; also reduced colonic damage and weight/length ratio in TNBS-induced colitis model [339]. Ursolic acid and 2α,3β,23-trihydroxyurs-12-en-28-oic acid reduced TPA-induced ear oedema and inhibited MPO activity [242].…”

Section: Scoparia Dulcismentioning

confidence: 99%

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Indigenous Uses, Phytochemical Analysis, and Anti-Inflammatory Properties of Australian Tropical Medicinal Plants

Yeshi

Turpin

Jamtsho³

et al. 2022

Molecules

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Australian tropical plants have been a rich source of food (bush food) and medicine to the first Australians (Aboriginal people), who are believed to have lived for more than 50,000 years. Plants such as spreading sneezeweed (Centipeda minima), goat’s foot (Ipomoea pes-caprae), and hop bush (Dodonaea viscosa and D. polyandra) are a few popular Aboriginal medicinal plants. Thus far, more than 900 medicinal plants have been recorded in the tropical region alone, and many of them are associated with diverse ethnomedicinal uses that belong to the traditional owners of Aboriginal people. In our effort to find anti-inflammatory lead compounds in collaboration with Aboriginal communities from their medicinal plants, we reviewed 78 medicinal plants used against various inflammation and inflammatory-related conditions by Aboriginal people. Out of those 78 species, we have included only 45 species whose crude extracts or isolated pure compounds showed anti-inflammatory properties. Upon investigating compounds isolated from 40 species (for five species, only crude extracts were studied), 83 compounds were associated with various anti-inflammatory properties. Alphitolic acid, Betulinic acid, Malabaric acid, and Hispidulin reduced proinflammatory cytokines and cyclooxygenase enzymes (COX-1 and 2) with IC50 values ranging from 11.5 to 46.9 uM. Other promising anti-inflammatory compounds are Brevilin A (from Centipeda minima), Eupalestin, and 5′-methoxy nobiletin (from Ageratum conyzoides), Calophyllolide (from Calophyllum inophyllum), and Brusatol (from Brucea javanica). D. polyandra is one example of an Aboriginal medicinal plant from which a novel anti-inflammatory benzoyl ester clerodane diterpenoid compound was obtained (compound name not disclosed), and it is in the development of topical medicines for inflammatory skin diseases. Medicinal plants in the tropics and those associated with indigenous knowledge of Aboriginal people could be a potential alternative source of novel anti-inflammatory therapeutics.

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Section: Anti-inflammatory Crude Extractsmentioning

confidence: 99%

Section: Scoparia Dulcismentioning

confidence: 99%

Indigenous Uses, Phytochemical Analysis, and Anti-Inflammatory Properties of Australian Tropical Medicinal Plants

Yeshi

Turpin

Jamtsho³

et al. 2022

Molecules

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“…Hemolysis of RBC in a hyposaline solution results in the release of internal hemoglobin into the surrounding solution. Hence, the protection or stabilization of the RBC membrane by anti-in ammatory drugs during hyposaline-induced hemolysis is necessary(Daram et al 2021). In the current investigation, ETSL exhibited the membrane stabilization potential in a concentration-dependent way.…”

mentioning

confidence: 99%

Protective effect of Tecomastans (L.) Juss.exKunth in CFA-induced arthritic rat model

Das,

Kar,

Dash

et al. 2024

Preprint

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Tecoma stans (L.) Juss.exKunth (Bignoniaceae) is mainly found in tropical and subtropical regions of Africa and Asia. The leaves, flowers, roots, and bark are used to treat various aliments includes, skin infections, kidney problems, intestinal disorders, jaundice, toothaches, joint pain and repair cracked bones, antidotes for snake, scorpion, and rat bites. The aim of the study is to assess the anti-arthritic properties of T. stansleaf using Complete Freund's adjuvant (CFA)-induced rat model. The ethanol extract of T. stansleaf (ETSL) was taken for Gas Chromatography-Mass Spectrometry (GC-MS) and Liquid Chromatography-Mass Spectrometry (LC-MS) analysis for the identification of potential bioactive. The in vitro antioxidant and anti-arthritic activity was studied at concentrations of 25, 50, 100, 200, 400, and 500 μg/ml. In vivo anti-arthritic activity was carried out by administering CFA (0.1 ml) into the sub-plantar surface of the right hind paw. The experimental animals were treated with indomethacin (10 mg/kg) and ETSL (250, 500 mg/kg) once a daily for fourteen days. The arthritic parameters such as paw thickness, arthritic index, arthritic score, body weight, organ weight, and hematological and biochemical parameters were evaluated. Pro-inflammatory cytokines; tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), IL-1β, anti-inflammatory cytokines; IL-4 and IL-10 and inflammatory mediator cyclooxygenase-2 (COX-2) were examined in blood serum. In vivo antioxidants parameters; superoxide dismutase (SOD), catalase (CAT), reduced glutathione (GSH), and lipid peroxidation (LPO) was carried out in liver and joint. Radiological and histopathological analysis of joint was performed.A computational molecular docking investigation of the phytoconstituents was conducted against COX-2, IL-1β, IL-6, and TNF-α receptors by utilizing AutoDock 4.2 and BIOVIA-Discovery Studio Visualizer software. The in vitro result showed concentration dependent antioxidant activity with highest percentage of inhibition at 500 µg/ml. The in vivo result demonstrated significant restoration of arthritic parameters, hematological and biochemical indices and oxidative stress in CFA-induced rat which was further supported by radiological histological examination at ETSL 500 mg/kg. In addition, there was significant (p<0.05) reduction in pro-inflammatory cytokines, inflammatory mediators and up-regulation of anti-inflammatory cytokines was observed in the treated group. Verbascoside was found to exhibit better biding affinities -10.4, -7.4, -7 and -6.2 kcal/mol against COX-2, IL-1β, TNF-α, and IL-6 respectively, confirmed through in silico study. The observed outcome suggests that ETSL at a dosage of 500 mg/kg demonstrated notable anti-arthritic effects by suppressing pro-inflammatory cytokines and oxidative stress biomarkers. This effect could potentially be attributed to the presence of bioactive verbascoside identified in the LC-MS analysis.

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Control release of α-mangostin by a novel dual-polysaccharides delivery system for colitis treatment under simulated gastrointestinal conditions

Zeng

Huang

et al. 2023

Food Bioscience

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Investigation of anti-inflammatory and anti-arthritic potentials of Terminalia catappa bark using in vitro assays and carrageenan-induced inflammation, complete Freund's adjuvant induced arthritis model in rats

Cited by 11 publications

References 24 publications

Indigenous Uses, Phytochemical Analysis, and Anti-Inflammatory Properties of Australian Tropical Medicinal Plants

Indigenous Uses, Phytochemical Analysis, and Anti-Inflammatory Properties of Australian Tropical Medicinal Plants

Protective effect of Tecomastans (L.) Juss.exKunth in CFA-induced arthritic rat model

Control release of α-mangostin by a novel dual-polysaccharides delivery system for colitis treatment under simulated gastrointestinal conditions

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