Investigation of an outbreak of sheeppox among native sheep breeds in the Western Himalayas of India

Chahota, Rajesh; Sharma, Pardeep; Kumar, Rajinder; Gupta, Tania Das; Sharma, Mukut

doi:10.1007/s11259-021-09833-z

Cited by 3 publications

(3 citation statements)

References 17 publications

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“… 2016 ; Chahota et al. 2022 ). In endemic regions, the morbidity rates in sheep and goats can reach 50% in adults and 100% in young animals, and the mortality rate can reach up to 50% (Zhou et al.…”

Section: Introductionmentioning

confidence: 99%

“… 2014 ; Chahota et al. 2022 ). The RPO30 gene, a close homolog of the E4L gene of vaccinia virus (VACV), encodes a 30 kDa DNA-dependent RNA polymerase subunit and it is located in the central region of the SPPV genome (ORF 036), known to play an important role in RNA replication (Lamien et al.…”

Section: Introductionmentioning

confidence: 99%

“… 2013 ; Chahota et al. 2022 ) and could potentially assist in designing safer vaccines, antiviral agents and identifying recombinant proteins for the development of diagnostic assays for the detection of SPPV and GTPV.…”

Section: Introductionmentioning

confidence: 99%

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Structural and sequence analysis of the RPO30 gene of sheeppox and goatpox viruses from India

Manjunatha Reddy,

Sumana,

Yogisharadhya

et al. 2024

Veterinary Quarterly

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Sheeppox and goatpox are transboundary viral diseases of sheep and goats that cause significant economic losses to small and marginal farmers worldwide, including India. Members of the genus Capripoxvirus (CaPV), namely Sheeppox virus (SPPV), Goatpox virus (GTPV), and Lumpy skin disease virus (LSDV), are antigenically similar, and species differentiation can only be accomplished using molecular approaches. The present study aimed to understand the molecular epidemiology and host specificity of SPPV and GTPV circulating in India through sequencing and structural analysis of the RNA polymerase subunit-30 kDa ( RPO30 ) gene. A total of 29 field isolates from sheep ( n = 19) and goats ( n = 10) belonging to different geographical regions of India during the period: Year 2015 to 2023, were analyzed based on the sequence and structure of the full-length RPO30 gene/protein. Phylogenetically, all the CaPV isolates were separated into three major clusters: SPPV, GTPV, and LSDV. Multiple sequence alignment revealed a highly conserved RPO30 gene, with a stretch of 21 nucleotide deletion in all SPPV isolates. Additionally, the RPO30 gene of the Indian SPPV and GTPV isolates possessed several species-specific conserved signature residues/motifs that could act as genotyping markers. Secondary structure analysis of the RPO30 protein showed four α-helices, two loops, and three turns, similar to that of the E4L protein of vaccinia virus (VACV). All the isolates in the present study exhibited host preferences across different states of India. Therefore, in order to protect vulnerable small ruminants from poxviral infections, it is recommended to take into consideration a homologous vaccination strategy.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Structural and sequence analysis of the RPO30 gene of sheeppox and goatpox viruses from India

Manjunatha Reddy,

Sumana,

Yogisharadhya

et al. 2024

Veterinary Quarterly

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Cytokine profile in lambs naturally infected with sheeppox virus

Karakurt,

Coskun,

Beytut

et al. 2023

Trop Anim Health Prod

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Pathological studies and ORF 103 gene based molecular characterization of sheep pox virus from outbreaks in Maharashtra, India

Dhaygude

BHUPESH

SHRUTI

et al. 2022

Indian J of Anim Sci

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Sheep and goat pox are endemic in India and causes considerable losses to the animal husbandry sector every year. There are multiple reports of this disease from different states of India; however, information on the occurrence of this disease in Maharashtra state is very scanty. In this context, the present investigation reports the outbreaks of sheep pox in Satara and Solapur districts of Maharashtra state, India, from August 2019 to February 2020 based on clinical signs, gross and histopathological lesions, and molecular detection. Additionally, the ORF 103 gene sequence analysis from field samples revealed 100 to 99% identity with SPPV from Egypt, China, Pune, Ahmedabad, and Makhdoom. In phylogeny, sequences of the present study grouped into SPPVs clade. The finding of this study adds to the knowledge of the epidemiology of sheep pox in Maharashtra state and may help in planning effective prevention and control strategies.

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Investigation of an outbreak of sheeppox among native sheep breeds in the Western Himalayas of India

Cited by 3 publications

References 17 publications

Structural and sequence analysis of the RPO30 gene of sheeppox and goatpox viruses from India

Structural and sequence analysis of the RPO30 gene of sheeppox and goatpox viruses from India

Cytokine profile in lambs naturally infected with sheeppox virus

Pathological studies and ORF 103 gene based molecular characterization of sheep pox virus from outbreaks in Maharashtra, India

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