Investigation of American lobster,Homarus americanus, for the presence of chlorinated dibenzo-p-dioxins and dibenzofurans

Introduction The aim of this study was to evaluate potential protective effects of propolis on furan-induced hepatic damage by assessing the levels of malondialdehyde (MDA) and reduced glutathione (GSH), antioxidant enzyme activities, and histopathological changes in the liver. Material and Methods Albino Wistar rats were divided into six groups: a control, propolis-treated (100 mg/kg b.w./day), low-dose furan-treated (furan-L group; 2 mg/kg b.w./day), high-dose furan-treated (furan-H group; 16 mg/kg b.w./day), furan-L+propolis treated, and furan-H+propolis treated group. Propolis and furan were applied by gavage; propolis for 8 days, and furan for 20 days in furan-L groups and 10 days in furan-H groups. ResultsWhile MDA levels were elevated in furan-treated groups, levels of GSH and activities of antioxidant enzymes decreased (p < 0.001). The levels of MDA and GSH and activities of antioxidant enzymes were normal in the furan+propolis groups, especially in the furan-L+propolis group (p < 0.001). While the aspartate transaminase, alanine transaminase, alkaline phosphatase, and lactate pdehydrogenase activities were elevated in the furan-H treated group (p < 0.05 and p < 0.001), they were unchanged in the furan-L treated group. Histopathologically, several lesions were observed in the liver tissues of the furan-treated groups, especially in the higher-dose group. It was determined that these changes were milder in both of the furan+propolis groups. Conclusion The results indicate that propolis exhibits good hepatoprotective and antioxidant potential against furan-induced hepatocellular damage in rats.

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“…Laboratory studies on animals have shown that furan is highly toxic even at low concentrations (9). Wilson .et al .…”

Section: Introductionmentioning

confidence: 99%

Protective role of propolis on low and high dose furan-induced hepatotoxicity and oxidative stress in rats

Kaya

Yılmaz

Çeribaşı

2019

Journal of Veterinary Research

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“…Composite samples have been used to measure contaminant concentrations from segmented populations including Great Lakes fish (e.g., ref 7), marine fish (e.g., ref 8), and birds (e.g., ref 9). However, many studies fail to describe how composite samples were formed (e.g., refs [10][11][12], and others provide descriptions of sample preparation techniques that have the potential for yielding biased results. Composites formed from identifiable units have unique characteristics and statistical properties that have not been addressed as thoroughly as those for composites formed from bulk samples.…”

Section: Introductionmentioning

confidence: 99%

Procedures for Formation of Composite Samples from Segmented Populations

Fabrizio¹,

Frank²,

Savino³

1995

Environ. Sci. Technol.

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“…1 2,3,7,8-Tetrachlorodibenzo-p-dioxin is considered the most toxic of the 75 PCDD congeners. 2 The PCDDs are extremely toxic requiring their measurement at pg g-' wet mass concentrations in foodstuffs. Polychlorinated dibenzofurans (PCDFs) are similar in source, structure and toxicity to PCDDs, with 2,3,7,8-tetrachlorodibenzofuran the most toxic of the 135 PCDF congeners;' PCDDs and PCDFs are present as trace contaminants in several industrial chemicals, e.g., pentachlorophenol, chlorophenoxy acids and chlorobenzenes, and are formed by incineration.'…”

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confidence: 99%

Rapid screening of fish tissue for polychlorinated dibenzo-p-dioxins and dibenzofurans

King

Uthe²,

Musial³

1993

Analyst

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A rapid, simple method for screening polychlorinated dibenzo-p-dioxin and dibenzofuran classes in shellfish tissue at pg g-1 wet mass concentrations is described. The method does not require a clean room facility and is based on saponification followed by extraction into hexane, clean-up using gel-permeation chromatography and sulfuric acid treatment, and measurement using capillary gas chromatography-low resolution mass spectrometry with selected ion monitoring. Selected ion monitoring using multiple ions eliminates interferences not removed by clean-up or chromatography. The detector response factor is constant for isomers within a class, e.g., tetrachlorodibenzo-p-dioxin isomers gave a mean response of 0.977 +/- 0.075 area counts fg-1, but varied significantly between classes. Thus one isomer serves as a 'standard' for all members of its class. Recoveries of added polychlorinated dibenzo-p-dioxins and dibenzofurans (20-500 pg g-1 wet mass) averaged 95.6 +/- 6.9 and 99.0 +/- 5.7%, respectively. The limits of detection (five times the noise level) are 20 pg g-1 tetrachlorodibenzo-p-dioxins and dibenzofurans, 20 pg g-1 pentachlorodibenzo-p-dioxins and dibenzofurans, 40 pg g-1 hexachlorodibenzo-p-dioxins and dibenzofurans, 40 pg g-1 heptachlorodibenzo-p-dioxins and dibenzofurans, and 100 pg g-1 octachlorodibenzo-p-dioxin and dibenzofuran. Above the limits of detection, the method gave results for polychlorinated dibenzo-p-dioxin and dibenzofuran classes in shellfish tissue comparable to those obtained by gas chromatography-high resolution mass spectrometry.

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Investigation of American lobster,Homarus americanus, for the presence of chlorinated dibenzo-p-dioxins and dibenzofurans

Cited by 6 publications

References 12 publications

Protective role of propolis on low and high dose furan-induced hepatotoxicity and oxidative stress in rats

Protective role of propolis on low and high dose furan-induced hepatotoxicity and oxidative stress in rats

Procedures for Formation of Composite Samples from Segmented Populations

Rapid screening of fish tissue for polychlorinated dibenzo-p-dioxins and dibenzofurans

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