Investigation of airborne fungi at different altitudes in Shenzhen University

Li, Li; Lei, Chao; Liu, Zhigang

doi:10.4236/ns.2010.25063

Cited by 14 publications

(11 citation statements)

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“…The 4 analyzed urban districts, each comprising a green and a busy site, showed a high airborne fungal diversity, which resulted in the isolation of 560 strains belonging to 110 fungal species in 49 genera. Previous studies have shown the presence of less diverse culturable airborne fungal communities, compare to Tianjin, in other cities of China, such as Beijing, where 40 species belonging to 14 genera were recorded [ 26 ], Hangzhou, with 85 species in 21 genera [ 40 ], and Shenzhen (27 species, 18 genera) [ 46 ]. Overall, a fungal concentration ranging from 0 to 3224.13 CFU m − 3 was recorded across the eight different analyzed sites in Tianjin.…”

Section: Discussionmentioning

confidence: 99%

“…Air samples were collected monthly, when the weather was dry and stable, approximately between 12:00–13:00 o’clock. Airborne fungi were sampled using the open plate method [ 2 , 46 , 75 ], by opening sterile plates containing media for 10 min at a height of 1.5 m above ground, which is representative of the human breathing zone. Minimum distance of 1 m from walls and obstacles was maintained.…”

Section: Methodsmentioning

confidence: 99%

“…Numerous scientific studies have been conducted on outdoor microorganisms in China and reported on national and international forums over the past several years. Airborne fungi concentrations are monitored in many Chinese cities, including Baoding [ 41 ], Beijing [ 26 , 42 – 44 ], Hangzhou [ 40 ], Nanjing [ 45 ], Shenzhen [ 46 ], Tainan [ 1 , 47 ], and Xi’an [ 48 , 49 ]. These studies have increased our basic knowledge on the diversity of airborne fungi in the environment and have provided data on some important issues related to public health [ 40 ].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of culturable airborne fungi in outdoor environments in Tianjin, China

et al. 2021

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Background Fungal spores dispersed in the atmosphere may become cause of different pathological conditions and allergies for human beings. A number of studies have been performed to analyze the diversity of airborne fungi in different environments worldwide, and in particular in many urban areas in China. We investigated, for the first time, the diversity, concentration and distribution of airborne fungi in Tianjin city. We sampled 8 outdoor environments, using open plate method, during a whole winter season. Isolated fungi were identified by morphological and molecular analysis. Environmental factors which could influence the airborne fungi concentration (temperature, humidity, wind speed, and air pressure) were monitored and analyzed. The effect of different urban site functions (busy areas with high traffic flow and commercial activities vs. green areas) on airborne fungal diversity was also analyzed. Results A total of 560 fungal strains, belonging to 110 species and 49 genera of Ascomycota (80 %), Basidiomycota (18 %), and Mucoromycota (2 %) were isolated in this study. The dominant fungal genus was Alternaria (22 %), followed by Cladosporium (18.4 %), Naganishia (14.1 %), Fusarium (5.9 %), Phoma (4.11 %), and Didymella (4.8 %). A fungal concentration ranging from 0 to 3224.13 CFU m− 3 was recorded during the whole study. Permutational multivariate analysis showed that the month was the most influential factor for airborne fungal community structure, probably because it can be regarded as a proxy of environmental variables, followed by wind speed. The two analyzed environments (busy vs. green) had no detectable effect on the air fungal community, which could be related to the relatively small size of parks in Tianjin and/or to the study season. Conclusions Our study shed light on the highly diverse community of airborne fungi characterizing the outdoor environments of Tianjin, and clarified the role that different environmental factors played in shaping the analyzed fungal community. The dominant presence of fungi with potential hazardous effect on human health, such as Alternaria, Cladosporium and Naganishia, deserves further attention. Our results may represent a valuable source of information for air quality monitoring, microbial pollution control, and airborne diseases prevention.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of culturable airborne fungi in outdoor environments in Tianjin, China

et al. 2021

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“…Direct quantification can be performed either by conventional microbiological methods (e.g. spore counting or determining the number of colony forming units, CFUs) (Li et al, 2010;Takahashi, 1997), or by nucleic acid-based analyses (e.g. quantitativePolymerase Chain Reaction (q-PCR) of extracted DNA) (Fröhlich-Nowoisky et al, 2009;Hospodsky et al, 2010;Yamamoto et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Annual distribution of allergenic fungal spores in atmospheric particulate matter in the Eastern Mediterranean; a comparative study between ergosterol and quantitative PCR analysis

et al. 2012

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Abstract. Airborne fungal spores are an important fraction of atmospheric particulate matter and are major causative agents of allergenic and infectious diseases. Predicting the variability and species of allergy-causing fungal spores requires detailed and reliable methods for identification and quantification. There are diverse methods for their detection in the atmosphere and in the indoor environments; yet, it is important to optimize suitable methods for characterization of fungal spores in atmospheric samples. In this study we sampled and characterized total and specific airborne fungal spores from PM10 samples collected in Rehovot, Israel over an entire year. The total fungal spore concentrations vary throughout the year although the species variability was nearly the same. Seasonal equivalent spore concentrations analyzed by real-time quantitative-PCR-based methods were fall > winter > spring > summer. Reported concentrations based on ergosterol analysis for the same samples were and fall > spring > winter > summer. Correlation between the two analytical methods was found only for the spring season. These poor associations may be due to the per-spore ergosterol variations that arise from both varying production rates, as well as molecular degradation of ergosterol. While conversion of genome copies to spore concentration is not yet straightforward, the potential for improving this conversion and the ability of qPCR to identify groups of fungi or specific species makes this method preferable for environmental spore quantification. Identifying tools for establishing the relation between the presence of species and the actual ability to induce allergies is still needed in order to predict the effect on human health.

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“…Direct quantification can be performed either by conventional microbiological methods (e.g. spore counting or determining the number of colony forming units, CFUs) (Li et al, 2010;Takahashi, 1997), or by nucleic acid-based analyses (e.g. quantitative-Polymerase Chain Reaction (q-PCR) of extracted DNA) (Fröhlich-Nowoisky et al, 2009;Hospodsky et al, 2010;Yamamoto et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Annual distribution of allergenic fungal spores in atmospheric particulate matter in the eastern mediterranean; a comparative study between ergosterol and quantitative PCR analysis

Lang‐Yona¹,

Dannemiller²,

Yamamoto³

et al. 2011

Preprint

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Airborne fungal spores are an important fraction of atmospheric particulate matter and are major causative agents of allergenic and infectious diseases. Predicting the variability and species of allergy-causing fungal spores requires detailed and reliable methods for identification and quantification. There are diverse methods for their detection in the atmosphere and in the indoor environments; yet, it is important to optimize suitable methods for characterization of fungal spores in atmospheric samples. In this study we sampled and characterized total and specific airborne fungal spores from PM<sub>10</sub> samples collected in Rohovot, Israel over an entire year. The total fungal spore concentrations vary throughout the year although the species variability was nearly the same. Seasonal equivalent spore concentrations analyzed by real-time quantitative-PCR-based methods were fall > winter > spring > summer. Reported concentrations based on ergosterol analysis for the same samples were and fall > spring > winter > summer. Correlation between the two analytical methods was found only for the spring season. These poor associations may be due to the per-spore ergosterol variations that arise from both varying production rates, as well as molecular degradation of ergosterol. While conversion of genome copies to spore concentration is not yet straightforward, the potential for improving this conversion and the ability of qPCR to identify groups of fungi or specific species makes this method preferable for environmental spore quantification. Identifying tools for establishing the relation between the presence of species and the actual ability to induce allergies is still needed in order to predict the effect on human health

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Investigation of airborne fungi at different altitudes in Shenzhen University

Cited by 14 publications

References 12 publications

Analysis of culturable airborne fungi in outdoor environments in Tianjin, China

Analysis of culturable airborne fungi in outdoor environments in Tianjin, China

Annual distribution of allergenic fungal spores in atmospheric particulate matter in the Eastern Mediterranean; a comparative study between ergosterol and quantitative PCR analysis

Annual distribution of allergenic fungal spores in atmospheric particulate matter in the eastern mediterranean; a comparative study between ergosterol and quantitative PCR analysis

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