Investigation of a small-diameter decellularised artery as a potential scaffold for vascular tissue engineering; biomechanical evaluation and preliminary cell seeding

Campbell, E.M.; Cahill, Paul A.; Lally, Caitríona

doi:10.1016/j.jmbbm.2012.06.001

Cited by 38 publications

(29 citation statements)

References 30 publications

(71 reference statements)

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“…The Darcy's permeability coefficient of the 70/30 Alg/Gel coated wadding are comparable to values reported in the literature, in the range of 1.2 × 10 −16 to 3 × 10 −12 m 2 , valid for decellularized arteries, human umbilical veins, and synthetic porous scaffolds, such as polycaprolactone, silk fibroin, and PGA with PLA copolymers [54][55][56][57]. This evidence confirms the coating was efficient to prevent leakage under low-pressure flow (16 mmHg) and shear stress of about 25 dyne/cm 2 .…”

Section: Discussionsupporting

confidence: 84%

Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering

Tresoldi

Pacheco

Formenti

et al. 2019

Materials Science and Engineering: C

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Aiming to perfuse porous tubular scaffolds for vascular tissue engineering (VTE) with controlled flow rate, prevention of leakage through the scaffold lumen is required. A gel coating made of 8% w/v alginate and 6% w/v gelatin functionalized with fibronectin was produced using a custom-made bioreactor-based method. Different volumetric proportions of alginate and gelatin were tested (50/50, 70/30, and 90/10). Gel swelling and stability, and rheological, and uniaxial tensile tests reveal superior resistance to the aggressive biochemical microenvironment, and their ability to withstand physiological deformations (~10%) and wall shear stresses (5-20 dyne/cm 2). These are prerequisites to maintain the physiologic phenotypes of vascular smooth muscle cells and endothelial cells (ECs), mimicking blood vessels microenvironment. Gels can induce ECs proliferation and colonization, especially in the presence of fibronectin and higher percentages of gelatin. The custom-designed bioreactor enables the development of reproducible and homogeneous tubular gel coating. The permeability tests show the effectiveness of tubular scaffolds coated with 70/30 alginate/gelatin gel to occlude wadding pores, and therefore prevent leakages. The synthesized double-layered tubular scaffolds coated with alginate/gelatin gel and fibronectin represent both promising substrate for ECs and effective leakproof scaffolds, when subjected to pulsatile perfusion, for VTE applications.

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Section: Discussionsupporting

confidence: 84%

Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering

Tresoldi

Pacheco

Formenti

et al. 2019

Materials Science and Engineering: C

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“…Similar to this study, Kochova et al demonstrated that Triton could partially remove smooth muscle cells; however, it strengthened, rather than weakened, the mechanical stiffening of the arterial wall . On the contrary, Campbell et al found that decellularization treatment maintained the mechanical integrity of porcine coronary artery . The unique mechanical properties of the artery are obtained by the coordinated interaction between the ECM and smooth muscle cells.…”

Section: Discussionsupporting

confidence: 46%

“…62 On the contrary, Campbell et al found that decellularization treatment maintained the mechanical integrity of porcine coronary artery. 63 The unique mechanical properties of the artery are obtained by the coordinated interaction between the ECM and smooth muscle cells. Furthermore, the results of the present study confirmed that the decrease of mechanical properties was closely related to the removal of smooth muscle cells and disruption of elastin.…”

Section: Discussionmentioning

confidence: 99%

Determining the optimal protocol for preparing an acellular scaffold of tissue engineered small‐diameter blood vessels

Pan

et al. 2017

J Biomed Mater Res

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Although detergent-based decellularization protocols have been widely used to obtain a natural extracellular matrix (ECM) scaffold in tissue engineering, some key challenges still exist. To achieve an optimum natural decellularized scaffold for the construction of tissue-engineered small-diameter blood vessels (TEBV), porcine carotid arteries (PCAs) were decellularized by combining sodium dodecyl sulfate (SDS), sodium deoxycholate (SDC) and Triton X-100 (Triton) in different concentrations. Tissue samples were processed and their histological, biochemical and biomechanical characteristics were investigated. Results showed that only two methods 0.5% (SDS + SDC) and 1% (SDS + SDC) could completely remove of the cellular contents and preserve the native ECM architecture. Furthermore, 1% (SDS + SDC) based methods acquire preferable porosity and suitable mechanical strength. Residual Triton in the ECM scaffold holds intensive cytotoxity. In conclusion, 1%(SDS + SDC) based method can obtain a superior PCAs scaffold for the construction of TEBV. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 619-631, 2018.

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“…14 They showed that the structure of ECM was preserved. Sodium hydroxide and Triton x100 following by SDS were used to decellularize ovine coronary arteries during 74 h. 15 In a recent study, sodium hydroxide was able to remove cells from porcine coronary arteries in a process that took 9 h. 16 Although decellularized vessels have been previously reported, the use of this technique for human small-diameter vessels is still limited. Schaner et al showed a decellularized human saphenous vein using 0.1% SDS for 15 h at 37 ° C. 17 They revealed that decellularized grafts have intact collagen morphology; however, their elastin staining decreased.…”

Section: Discussionmentioning

confidence: 99%

Decellularization of Human Internal Mammary Artery: Biomechanical Properties and Histopathological Evaluation

Kajbafzadeh

Khorramirouz

Kameli

et al. 2017

BioResearch Open Access

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This study undertook to create small-diameter vascular grafts and assess their structure and mechanical properties to withstand arterial implantation. Twenty samples of intact human internal mammary arteries (IMAs) were collected and decellularized using detergent-based methods. To evaluate residual cellular and extracellular matrix (ECM) components, histological analysis was performed. Moreover, collagen typing and ECM structure were analyzed by Picrosirius red and Movat's pentachrome staining. Scanning electron microscopy was also applied to assess microarchitecture of both endothelial and adventitial surfaces of native and decellularized arterial samples. Furthermore, mechanical tests were performed to evaluate the rigidity and suture strength of the arteries. Human IMAs were completely decellularized in all three segments (proximal, middle, and distal). ECM proteins such as collagen and elastic fibers were efficiently preserved and no structural distortion in intima, media, and adventitial surfaces was observed. The parameters of the mechanical tests revealed no significant differences in the mechanical properties of decellularized arteries in comparison to native arteries with considerable strength, suture retention, and stress relaxation (Young's modulus [MPa] = 0.22 ± 0.023 [native] and 0.22 ± 0.015 [acellular]; and suture strength 0.56 ± 0.19 [native] vs. 0.56 ± 0.12 [acellular], respectively). Decellularized IMA represents a potential arterial scaffold as an alternative to autologous grafts for future arterial bypass surgeries. By this technique, microarchitecture and mechanical integrity of decellularized arteries were considerably similar to native arteries. The goal of this study was to introduce an efficient method for complete decellularization of human IMA and evaluate the ECM and biomechanical properties.

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Investigation of a small-diameter decellularised artery as a potential scaffold for vascular tissue engineering; biomechanical evaluation and preliminary cell seeding

Cited by 38 publications

References 30 publications

Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering

Shear-resistant hydrogels to control permeability of porous tubular scaffolds in vascular tissue engineering

Determining the optimal protocol for preparing an acellular scaffold of tissue engineered small‐diameter blood vessels

Decellularization of Human Internal Mammary Artery: Biomechanical Properties and Histopathological Evaluation

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