Investigation of a capillary electrophoretic approach for direct quantification of apolipoprotein A‐I in serum

Abstract

In the present study a rapid, reproducible and robust capillary electrophoresis (CE) procedure for the quantification of apolipoprotein A-I (Apo A-I) in serum without pretreatment has been developed (total run time, 11 min). The coefficients of variation (CV; n = 10) for the relative peak area are 1.8% at a concentration of 145 mg/dL and 1.6% at 196 mg/dL; and for the inter-assay 8.9% at 161 mg/dL (10 consecutive days), i.e., similar to the CVs of a high-throughput immunonephelometric routine assay. The CV for the migration time is 0.4% (n = 20). The robustness of the CE approach was tested in patient samples with hemolysis, hyperbilirubinemia and hyperlipidemia. A comparison of 99 Apo A-I serum values with results of a fixed-time immunonephelometric routine assay showed a positive constant bias of 60% (mean) for the immunonephelometric values, no deviation from linearity, but significant deviations in several samples. Investigations on interferences in the CE analyses gave no evidence that CE failed. Our study shows that CE is amenable to a fast analysis and a reproducible and reliable quantification of Apo A-I level in sera of various clinical samples.

Capillary electrophoresis of proteins 2003–2005

“…Apolipoprotein A-I was quantified in human serum by CZE without any pretreatment [205]. The robustness of the method was confirmed by the analysis of hemolytic, hyperbilirubinemic, and hypelipoproteinemic sera, and no or low interference was observed.…”

A Simple Label-Free Aptamer-Based Electrochemical Biosensor for the Sensitive Detection of C-Reactive Proteins

“…The current detection methods for CRP include enzyme-linked immunosorbent (ELISA) [ 21 ], latex-enhanced immunoturbidimetry [ 22 ], single radial immunodiffusion (SRID) [ 23 , 24 ], and immunoscattering turbidimetry [ 25 , 26 ]. However, the limitations of these techniques are also evident: They are vulnerable to interference, time consuming, expensive instruments, and require complex operations, and they are particularly inadequate in the clinical development of effective treatment strategies and timely and rapid CRP detections [ 27 ].…”