Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis

Bradbury, W C; Pearson, Andrew D.J.; Marko, M A; Congi, R V; Penner, J L

doi:10.1128/jcm.19.3.342-346.1984

Cited by 89 publications

(29 citation statements)

References 20 publications

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“…We were not able to allocate antibiotic resistance or other phenotypic markers to these plasmids. Plasmid profiling has not been used extensively as an epidemiological typing tool for Campylobacter species (19), mainly because plasmids are recovered from clinical isolates of C. jejuni at low rates (5). If it is confirmed by other investigators that "C. upsaliensis" is quite different from C. jejuni, in that a high proportion of strains contain multiple plasmids, then plasmid profiling might become a useful marker for distinguishing "C.…”

Section: Discussionmentioning

confidence: 99%

Characterization and description of "Campylobacter upsaliensis" isolated from human feces

Goossens¹,

Pot²,

Vlaes³

et al. 1990

J Clin Microbiol

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During a 3-year period, "Campylobacter upsaliensis" was isolated from 99 patients. Phenotypic characterization and numerical analysis of protein electrophoregrams showed evidence that "C. upsaliensis" is a distinct Campylobacter species with unique characteristics. The MBCs of 13 antibiotics were determined. In general, these organisms were highly susceptible to drugs that were present in the selective isolation media, making none of the available selective media suitable for the isolation of "C. upsaliensis." Ten strains were found to be resistant to erythromycin (MBCs, .12.50 mg/liter). Plasmid DNA was detectable in 89 of the 99 strains; 16 plasmid profiles could be identified. Plasmid pattern 16, containing four plasmids of 52, 32, 5.5, and 2.6 megadaltons, represented 60.7% of the plasmid-containing strains. None of the "C. upsaliensis" strains could be agglutinated with antisera against heat-labile antigens from C. jejuni, C. coli, or C. laridis. "C. upsaliensis" was found to be susceptible to serum killing and was readily phagocytized by human polymorphonuclear cells. Catalase-negative or weakly positive (CNW) strains of Campylobacter were first isolated from dogs by Sandstedt et al. (31). In the same report, those investigators were able to demonstrate, by DNA hybridization, that these organisms belonged to a new species; the name "Campylobacter upsaliensis" was proposed in 1986 (K. Sandstedt and J. Ursing, Abstr. XIV Int. Congr. Microbiol. P.B8-17, p. 61, 1986). The species description was minimal, although a type strain (NCTC 11541 = LMG 8850) was designated. Neither the species name nor the type strain has standing in the literature, because the species description was not published in a peer-reviewed journal. CNW strains were isolated from five children with diarrhea by Mégraud and Bonnet in France (F. Mégraud and F. Bonnet, Letter, J. Infect. 12:275-276, 1986); Steele and McDermott (32) recovered eight CNW isolates from diarrheic stools of Australian children and one CNW isolate from an adult; Patton et al. (27) showed evidence that "C. upsaliensis" is responsible for enteritis in both adults and children in the United States; Taylor et al. (36) isolated "C. upsaliensis" from seven patients, both adults and children, with diarrhea in Canada; Lastovica et al. (16) isolated these organisms from blood cultures of pediatric patients in South Africa; Walmsley and Karmali (39) isolated "C. upsaliensis" from six children. Most recently, Fox et al. (8) isolated "C. upsaliensis" from three asymptomatic cats. In this report, we characterize and describe the "C. upsaliensis" group, using strains isolated in our hospital. We use the species name in quotation marks to indicate its proposed name and refer to the "type strain" as a reference strain.

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Section: Discussionmentioning

confidence: 99%

Characterization and description of "Campylobacter upsaliensis" isolated from human feces

Goossens¹,

Pot²,

Vlaes³

et al. 1990

J Clin Microbiol

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“…However, the limited availability of adequate strain markers has curtailed studies aimed at elucidating the sources and modes of transmission of this organism. Procedures defining unique serotypes (1,19,26,28,30), biotypes (6,18), plasmid profiles (34), and chromosomal restriction patterns (7) have all been used to identify epidemic-causing strains. The usefulness of these techniques, however, has been limited by their complexity and limited availability.…”

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confidence: 99%

Use of auxotyping for epidemiological studies of Campylobacter jejuni and Campylobacter coli infections

Tenover¹,

Knapp²,

Patton³

et al. 1985

Infect Immun

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A chemically defined medium developed for Neisseria gonorrhoeae was modified to support the growth of Campylobacterjejuni and Campylobacter coli. A total of 76 isolates of C. jejuni and 14 isolates of C. coli were tested on this medium, which was designated Campylobacter defined medium (CDM), over a 3-month period. Although none of the C. coli isolates appeared to require amino acids, 51% of the C. jejuni tested required one and 7% required multiple amino acids for growth. An analysis of isolates obtained from three household outbreaks of campylobacteriosis demonstrated that auxotyping identified the epidemic strain within each outbreak. Among 70 isolates of C. jejuni examined, no correlation could be drawn between a specific serotype and auxotype or between auxotype and plasmid profile.

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“…Recently, serotyping and chromosomal DNA restriction enzyme analysis have been used successfully to investigate water-or milk-borne C. jejuni and C. coli outbreaks (4,8,13,19). Strains of the same genotype would be expected to have the same restriction patterns, whereas strains of different genotypes could possess genes encoding the biosynthesis of the same thermostable antigenic specificity.…”

Section: Discussionmentioning

confidence: 99%

Investigation of zoonotically acquired Campylobacter jejuni enteritis with serotyping and restriction endonuclease DNA analysis

et al. 1989

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Five strains of Campylobacter jejuni were isolated from asymptomatic coyotes being maintained in a vivarium. According to the results of serotyping with thermostable antigens and of bacterial chromosomal restriction endonuclease analysis, one of the C. jejuni strains isolated from the coyotes was responsible for enteritis in a laboratory animal technician providing husbandry for the laboratory-maintained coyotes. Although zoonotic spread of Campylobacter jejuni has been linked to pet animals, detailed analysis of the human and animal C. jejuni strains isolated in these outbreaks to substantiate animal-to-human transmission of the organism has not been reported (1-3; M.

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Investigation of a Campylobacter jejuni outbreak by serotyping and chromosomal restriction endonuclease analysis

Cited by 89 publications

References 20 publications

Characterization and description of "Campylobacter upsaliensis" isolated from human feces

Characterization and description of "Campylobacter upsaliensis" isolated from human feces

Use of auxotyping for epidemiological studies of Campylobacter jejuni and Campylobacter coli infections

Investigation of zoonotically acquired Campylobacter jejuni enteritis with serotyping and restriction endonuclease DNA analysis

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