Investigation of a Broad-Spectrum PCR Assay for Human Papillomaviruses in Screening Benign Lesions of the Upper Aerodigestive Tract

Fischer, Markus

doi:10.1159/000089347

Cited by 6 publications

(6 citation statements)

References 29 publications

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“…Included are both case reports and larger series, comprising up to 101 samples analyzed by PCR16 and 67 papillomas examined by ISH110 (Table I). The methods used to evaluate the HPV involvement include the following: IHC,31 DB,51, 52 SB,41, 43, 45, 50, 55, 60, 69, 76 ISH,11, 32, 42, 44, 46, 47, 49, 56, 65, 70, 94, 103, 106, 108, 110, 114, 117, 120 and PCR 13, 15–18, 48, 53, 54, 57, 58, 59, 61–64, 66–68, 71–75, 77–93, 95–102, 104, 105, 107, 109, 111–113, 115, 116, 118, 119, 121. The studies were categorized into the following regions of origin: China and Taiwan, Other Asia, Africa/Middle East, Europe, North America, and South America.…”

Section: Resultsmentioning

confidence: 99%

Detection of human papillomavirus in sinonasal papillomas: Systematic review and meta‐analysis

2012

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Section: Resultsmentioning

confidence: 99%

Detection of human papillomavirus in sinonasal papillomas: Systematic review and meta‐analysis

2012

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“…The protein-DNA and DNA-DNA cross-links formed with formalin fixation hamper DNA extraction for the initial steps of PCR. Only two PCR based studies used prospectively collected frozen tissue samples [32,35] with differing detection rates (4/6 or 67% and 3/26 or 12% respectively).…”

Section: Methodology Does Not Impact Hpv Detection Rates For Ipmentioning

confidence: 99%

The Role of the Human Papillomavirus in the Pathogenesis of Schneiderian Inverted Papillomas: An Analytic Overview of the Evidence

Lawson

Schlecht

Brandwein-Gensler

2008

Head and Neck Pathol

164

167

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We hypothesize that LR HPV may induce IP formation, and then are lost as infected cells are shed, as a "hit and run" phenomenon. HPV detection rates increase in dysplastic IP and SCC-ex-IP with increasing ratio of HR to LR HPV types, compared to nondysplastic IP. We believe that one explanation for the variation in HPV detection rates between different studies may be the actual histologic composition of the cohort. That is, if one series contains a higher frequency of dysplastic and malignant IP, it may have a higher detection rate than another series which contains only nondysplastic IP. We hypothesize that the higher rates of HPV detection in dysplastic and malignant IP may be related to HPV integration. The implication of this is that HPV sub-type testing may identify patients at risk for recurrence, or progression to dysplasia and malignancy, and thus may impact surveillance protocols.

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“…In oral benign lesions associated with HPV, a prevalence of HPV 6 and 11 was observed in squamous cell papilloma (SCP) and condyloma acuminatum, while HPV 2 and 57 were more prevalent in verruca vulgaris lesions [ 59 ]. Fischer et al suggested that the applied broad-spectrum PCR system is a reliable tool in the detection of HPV DNA in benign lesions of the upper aerodigestive tract [ 60 ]. Hoffmann et al suggested that HPV is correlated to a lesser extent to infectious mucosal lesions than to proliferative lesions.…”

Section: Introductionmentioning

confidence: 99%

Role of human papillomavirus and its detection in potentially malignant and malignant head and neck lesions: updated review

et al. 2009

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Head and neck malignancies are characterized by a multiphasic and multifactorial etiopathogenesis. Tobacco and alcohol consumption are the most common risk factors for head and neck malignancy. Other factors, including DNA viruses, especially human papilloma virus (HPV), may also play a role in the initiation or development of these lesions. The pathways of HPV transmission in the head and neck mucosal lesions include oral-genital contact, more than one sexual partner and perinatal transmission of HPV to the neonatal child. The increase in prevalence of HPV infection in these lesions may be due to wider acceptance of oral sex among teenagers and adults as this is perceived to be a form of safe sex. The prevalence of HPV in benign lesions as well as malignancies has been assessed by many techniques. Among these, the polymerase chain reaction is the most sensitive method. Review of literature reveals that HPV may be a risk factor for malignancies, but not in all cases. For confirmation of the role of HPV in head and neck squamous cell carcinoma, large population studies are necessary in an assortment of clinical settings. Prophylactic vaccination against high-risk HPV types eventually may prevent a significant number of cervical carcinomas. Of the two vaccines currently available, Gardasil ® (Merck & Co., Inc.) protects against HPV types 6, 11, 16 and 18, while the other vaccine, Cervarix ® (GlaxoSmithKline, Rixensart, Belgium) protects against HPV types 16 and 18 only. However, the HPV vaccine has, to the best of our knowledge, not been tried in head and neck carcinoma. The role of HPV in etiopathogenesis, prevalence in benign and malignant lesions of this area and vaccination strategies are briefly reviewed here.

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Investigation of a Broad-Spectrum PCR Assay for Human Papillomaviruses in Screening Benign Lesions of the Upper Aerodigestive Tract

Cited by 6 publications

References 29 publications

Detection of human papillomavirus in sinonasal papillomas: Systematic review and meta‐analysis

Detection of human papillomavirus in sinonasal papillomas: Systematic review and meta‐analysis

The Role of the Human Papillomavirus in the Pathogenesis of Schneiderian Inverted Papillomas: An Analytic Overview of the Evidence

Role of human papillomavirus and its detection in potentially malignant and malignant head and neck lesions: updated review

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