Investigation of 3111T/C polymorphism of the CLOCK gene in obese individuals with or without binge eating disorder: Association with higher body mass index

Monteleone, Palmiero; Tortorella, Alfonso; Docimo, Ludovico; Maldonato, Mauro; Canestrelli, Benedetta; Luca, Luca De; Maj, Mario

doi:10.1016/j.neulet.2008.02.003

Cited by 68 publications

(53 citation statements)

References 14 publications

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“…Tortorella (Tortorella et al, 2007) observed an association of the C allele of this SNP with lifetime lower body weight in a group of subjects with eating disorders. Whereas Monteleone (Monteleone et al, 2008) did not find any association between the 3111T/C SNP and human obesity…”

Section: Introductionmentioning

confidence: 85%

Physical Activity and Sex Modulate Obesity Risk Linked to 3111T/C Gene Variant of theCLOCKGene in an Elderly Population: The SUN Project

Galbete

Contreras

Martínez

et al. 2012

Chronobiology International

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Genetic factors may interact with physical activity levels to modify obesity risk. Our aim was to explore the effect of rs1801260 SNP (3111T/C) of CLOCK gene, on obesity risk and to examine their interaction with lifestyle factors in an elderly population of the SUN Project. Subjects (n=903, aged 69±6) were recruited from the SUN ("Seguimiento Universidad de Navarra") Project. DNA was obtained from saliva and lifestyle and dietary data were collected by validated self-reported questionnaires. Genotyping was assessed by RT-PCR plus allele discrimination. A significant interaction between the 3111T/C SNP of CLOCK gene and sex for overweight/obesity risk was observed (p for interaction [Sex*CLOCK] interaction <0.001). Our results showed that women carrying the C allele of CLOCK gene had a decreased overweight/obesity risk compared to non carrier -TT-subjects (OR 0.61, 95% CI: 0.36-1.04, p=0.069). Moreover, the protective effect of the 3111T/C gene variant may be enhanced in those women with a high physical activity practice. We found that women practicing more than 16.8 METsh/week had a significantly lower overweight/obesity risk (OR 0.36, 95%CI: 0.17-0.79, p=0.011). A significant interaction between the 3111T/C gene variant and physical activity for overweight/obesity risk was observed in women (p for [PA x CLOCK] interaction=0.015). In conclusion, it appears that physical activity levels may act as an effect modifier for overweight/obesity risk linked to the 3111T/C SNP (rs1801260) of the CLOCK gene in an elderly population of the SUN Project.

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Section: Introductionmentioning

confidence: 85%

Physical Activity and Sex Modulate Obesity Risk Linked to 3111T/C Gene Variant of theCLOCKGene in an Elderly Population: The SUN Project

Galbete

Contreras

Martínez

et al. 2012

Chronobiology International

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“…Although the resulting phenotype appears partially dependent on genetic background, mutation of clock-related genes results in metabolic dysregulation (Turek et al, 2005;Oishi et al, 2006;Green et al, 2007;Kennaway et al, 2007). Similar genetic links have been found in humans, with clock gene polymorphisms being associated with obesity, metabolic syndrome, type 2 diabetes mellitus and circadian rhythm sleep disorders (Woon et al, 2007;Monteleone et al, 2008;Scott et al, 2008;Sookoian et al, 2008;Pereira et al 2005;Lee et al, 2007;Pedrazzoli et al, 2007).…”

Section: Introductionmentioning

confidence: 86%

Circadian Rhythmicity in Murine Pre-Adipocyte and Adipocyte Cells

Otway

Frost

Johnston

2009

Chronobiology International

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Adipose tissue is central to metabolic homeostasis, signalling in part through the secretion of molecules termed adipokines. Circadian rhythms play an important role in adipose physiology, with plasma adipokine concentration and ~20% of the murine adipose transcriptome undergoing24 h variation. However, due to the heterogeneity of adipose tissue and rhythmical input from both neuronal and humoral signals, the cellular basis of adipose rhythms is unclear. We tested the hypothesis that adipocyte cells contain a circadian clock that drives rhythmic mRNA expression and adipokine secretion. From the murine preadipocyte 3T3-L1 cell line, we generated populations of both pre-adipocytes and differentiated adipocytes. Cells were then treated with a 2 h serum pulse and sampled every 4 h over a 48 h period. Expression of clock gene, 'metabolic' gene (PPARα, PPARγ, SREBP1) and adipokine mRNA was analyzed by quantitative real-time PCR, and secretion of the adipokines leptin and adiponectin was measured in culture medium from differentiated adipocytes. In pre-adipocytes, we observed robust rhythms of clock genes Per2, Rev-erbα, and Dbp, but not of Per1, Cry1, Bmal1, or any of the 'metabolic' genes. Adipocytes produced similar temporal profiles of mRNA expression, albeit with a markedly reduced amplitude of Per2 and Dbp rhythms. Despite no circadian rhythm of adipokine mRNA expression, leptin accumulation in the culture medium suggested circadian control of leptin secretion from adipocytes. Adiponectin secretion showed temporal variation, but without any apparent circadian rhythmicity. Our data, therefore, suggest that an endogenous adipocyte clock controls the rhythmic expression of only a subset of genes that are reported to exhibit 24 h rhythmicity in murine adipose tissue. Moreover, secretion of leptin may also be regulated by the adipocyte clock.

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“…The mechanisms underlying this phenomenon are still unclear, but the increased risk may, in part, be related to circadian misalignment (Knutsson & Boggild 2000). The circadian clock plays an important role in the regulation of endogenous metabolic processes (Woon et al 2007, Green et al 2008, Monteleone et al 2008, Scott et al 2008, Sookoian et al 2008. Under entrained or adapted circumstances, these endogenous processes are synchronised to or in phase with daily routines, such as food intake and sleep.…”

Section: Introductionmentioning

confidence: 99%

Effect of total sleep deprivation on postprandial metabolic and insulin responses in shift workers and non-shift workers

Wehrens¹,

Hampton²,

Finn³

et al. 2010

Journal of Endocrinology

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Epidemiological studies have shown that shift workers are at a greater risk of developing cardiovascular disease which may, in part, be related to metabolic and hormonal changes. Partial sleep deprivation, a common consequence of rotating shift work, has been shown to affect glucose tolerance and insulin sensitivity. The current study investigated the effects of one night of total sleep deprivation, as a proxy for the first night shift, on postprandial glucose, insulin and lipid (triacylglycerols (TAGs) and non-esterified fatty acids (NEFAs)) responses under controlled laboratory conditions in shift workers and non-shift workers. Eleven experienced shift workers (35 . 7G7 . 2 years, meanGS.D.) who had worked in shifts for 8 . 7G5 . 25 years were matched with 13 non-shift workers who had worked for 32 . 8G6 . 4 years. After an adaptation night and a baseline sleep night, volunteers were kept awake for 30 . 5 h, followed by a nap (4 h) and recovery sleep. Blood samples were taken prior to and after a standard breakfast following baseline sleep, total sleep deprivation and recovery sleep. Basal TAG levels prior to the standard breakfast were significantly lower after sleep deprivation, indicating higher energy expenditure. Basal NEFA levels were significantly lower after recovery sleep. Postprandial insulin and TAG responses were significantly increased, and the NEFA response was decreased after recovery sleep, suggestive of insulin insensitivity. Although there were no overall significant differences between non-shift workers and shift workers, non-shift workers showed significantly higher basal insulin levels, lower basal NEFA levels, and an increased postprandial insulin and a decreased NEFA response after recovery sleep. In future, the reasons for these inter-group differences are to be investigated.

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Investigation of 3111T/C polymorphism of the CLOCK gene in obese individuals with or without binge eating disorder: Association with higher body mass index

Cited by 68 publications

References 14 publications

Physical Activity and Sex Modulate Obesity Risk Linked to 3111T/C Gene Variant of theCLOCKGene in an Elderly Population: The SUN Project

Physical Activity and Sex Modulate Obesity Risk Linked to 3111T/C Gene Variant of theCLOCKGene in an Elderly Population: The SUN Project

Circadian Rhythmicity in Murine Pre-Adipocyte and Adipocyte Cells

Effect of total sleep deprivation on postprandial metabolic and insulin responses in shift workers and non-shift workers

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