Investigation into the Wound Healing Activity of Monodora myristica and Monodora tenuifolia Seed Extracts in Albino Rats

Ajayi, I. A.; Raji, Adewale; Umeh, Adonai

doi:10.9734/acsj/2015/19876

Cited by 2 publications

(5 citation statements)

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“…1 L of methanol was added to each of the bottles and left at room temperature of about 32 o C for five days. The contents were stirred continuously for proper mixing with the solvent and then filtered (Ajayi et al, 2015). The filtrate was distilled to recover the solvent from the oil.…”

Section: Methodsmentioning

confidence: 99%

Phytochemical Composition and Wound Healing Activity of Azadirachta Indica Seed Extract in Wistar Rats.

Ibironke¹,

Odejobi²

2021

AIMS Research Publication

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The wound healing activity, antimicrobial and phytochemical constituents of Azadirachta indica seeds were investigated. The methanolic extract of A. indica was prepared, labeled as AIME and studied for phytochemical constituents, antimicrobial activity on transient flora microorganisms of the skin using standard methods. Wound healing study was conducted on 28 Wistar rats distributed into four groups of seven rats each and incised with wound area of 550 mm2. Rats from Groups 1 and 2 were treated with 15 % and 20 % of AIME respectively, Group 3 with 100 % ointment (positive control) while Group 4 were left untreated (negative control). The wound healing activity of the prepared ointments were assessed by the rate of wound contraction, wound area and epitheliazation time on every four days interval. The wound effect on the rats (feed intake, growth parameters, change in body weight, and specific growth rate) were also assessed. The phytochemical analysis of AIME revealed the presence of different bioactive compounds such as alkaloid, reducing sugar, cardiac glycosides, flavonoids, terpenoids and sterols in AIME. The two prepared ointments exhibited significant antimicrobial activity on S. aureus, B. subtilis and P. aeroginosa. The results of the wound healing activity indicated that 15 % AIME had the most potent wound healing capacity as evident from epitheliazation time (15.50±0.55 days), followed by its 20 % counterpart with the epitheliazation time of 23.33±2.73 days; that of the positive control was 18.00±1.87. The activity of the endogenous enzymes on the scar areas were also found to be regulated by the seed extract. The increased wound contraction, the antioxidative and moderate antimicrobial activities of the methanolic extract are indicative of the wound healing potential of A. indica seeds. Keywords: Seed extract; phytochemical; antimicrobial; wound healing; epitheliazation period

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Section: Methodsmentioning

confidence: 99%

Phytochemical Composition and Wound Healing Activity of Azadirachta Indica Seed Extract in Wistar Rats.

Ibironke¹,

Odejobi²

2021

AIMS Research Publication

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“…The obtained seeds were dried and granulated to coarse powder with an electronic blender to build the yield of the concentrate. Around 500 g of dried powdered examples were placed in a suction tool bottle and 1 L hexane added to it at room temperature (30 °C) for 5 days with constant blending to permit it to mix appropriately with the solvent after which it was separated [13]. The filtrate was then refined to recover the solvent from the oil for additional examination.…”

Section: Preparationmentioning

confidence: 99%

“…The rate of wound contraction was determined as percentage reduction of size on a four-day basis. Gradual decrease in the wound size was obtained periodically using transparency paper and a maker, and the wound area was measured graphically to monitor the percentage of wound closure which indicates new epithelial tissue to cover the wound [13]. The percentage wound contraction was measured according to Srivastava and Durgaprasad (2008) formula:…”

Section: Rate Of Wound Contractionmentioning

confidence: 99%

“…The skin and internal organs specimens from the treated animals were collected in 10 % buffered formalin and were subjected to sectioning and 6 mm thickness sections were stained with hematoxylin and eosin. The stained slides were visualized for histological changes under a light microscope [13].…”

Section: Hispathological Analysismentioning

confidence: 99%

“…Packed cell volume, haemaglobin concentration, red blood cell and white blood cell counts were determined using standard techniques as described by Dacie and Lewis [20]. The differential WBC counts mean corpuscular volume and mean corpuscular haemaglobin concentration were calculated [13,21]. Microhaemocrit capillary tubes were filled to two-thirds mark with well mixed venous blood.…”

Section: Haematological Analysismentioning

confidence: 99%

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Wound Healing Activity of Neocarya macropyhlla Seed Extracts in Wistar Rats

Omolere

Ifedi

Ajayi

2022

BJI

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Neocarya macropuylla seed hexane extract (NMSHE) was examined for the presence of various phytoconstituents. The antimicrobial activity was evaluated by testing the extract on selected drug resistant bacteria viz: S. aureus, E. coli, B. subtilis, P. aeruginosa and fungi viz: C. albicans, A. niger using agar technique of pour plate and surface plate dilution that were selected based on their peculiarity to humans. Wound healing activity of NMSHE was investigated by formulating a dosage of 5 % and 10 % concentration of the extract in paraffin. 21 wistar rats shared into three groups with each group housing 7 animals each over an experimental period of 21 days were properly fed and given free access to water throughout the experiment that was authenticated by the weight measurement taken every four days. Investigation of the wound healing activity of the extract was performed by measuring the wound area (mm2) and percentage of wound closure on the 4th, 8th, 12th, 16th and 20th day of the study; the wound epitheliasation was determined from the 16th to 20th days. Histopathological analysis of the control and test groups’ skin tissues, liver, kidney, heart, lung and spleen and haematological evaluation of the blood parameters were carried out at the end of the experiment. The result of the phytochemical analysis revealed the presence of terpenoids and glycosides in NMSHE. At 5 % and 10 % concentrations, NMSHE inhibited the growth of E. coli, P. aeruginosa, S. aureus, B. subtilis, C. albicans and A. niger. Group 2 rats treated with 5 % NMSHE gave a faster and better epitheliasation time than those in group 1 treated with control base ointment. The histopathology and haematology analyses result showed no disparity between the control and the test group; this is a point of reference showing that the extracts seemed to possess some essential constituents which actually hastened the wound healing process. macrophylla seed hexane extract healed the wound created on wistar rats at a faster time than the control ointment hence could probably serve as a replacement to conventional wound healing ointment.

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Investigation into the Wound Healing Activity of Monodora myristica and Monodora tenuifolia Seed Extracts in Albino Rats

Cited by 2 publications

References 0 publications

Phytochemical Composition and Wound Healing Activity of Azadirachta Indica Seed Extract in Wistar Rats.

Phytochemical Composition and Wound Healing Activity of Azadirachta Indica Seed Extract in Wistar Rats.

Wound Healing Activity of Neocarya macropyhlla Seed Extracts in Wistar Rats

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