Investigation into the role of DNA methylation in cyanogenesis in sorghum (Sorghum bicolor L. Moench)

Rosati, Viviana C; Quinn, Alicia A.; Fromhold, Samantha Maree; Gleadow, Roslyn M.; Blomstedt, Cecilia K.

doi:10.1007/s10725-019-00489-z

Cited by 11 publications

(9 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The acdc1 line used in this study has a mutation in the promoter of CYP79A1 gene, the first step in the dhurrin biosynthesis pathway [ 41 ]. Based on the observation that this line is developmentally acyanogenic, i.e., dhurrin concentration decreased earlier in development and to a lower level compared with wild type plants, and that the mutation appears to be regulatory we hypothesized that this phenotype might be the result of altered phytohormone signalling.…”

Section: Resultsmentioning

confidence: 99%

Allocation of Resources to Cyanogenic Glucosides Does Not Incur a Growth Sacrifice in Sorghum bicolor (L.) Moench

Sohail

Blomstedt

Gleadow

2020

Plants

Self Cite

View full text Add to dashboard Cite

In plants, the production of secondary metabolites is considered to be at the expense of primary growth. Sorghum produces a cyanogenic glycoside (dhurrin) that is believed to act as its chemical defence. Studies have shown that acyanogenic plants are smaller in size compared to the wildtype. This study aimed to investigate whether the small plant size is due to delayed germination or due to the lack of dhurrin derived nitrogen. A novel plant system consisting of totally cyanide deficient class 1 (tcd1) and adult cyanide deficient 1 (acdc1) mutant lines was employed. The data for germination, plant height and developmental stage during seedling development and final plant reproductive fitness was recorded. The possible role of phytohormones in recovering the wildtype phenotype, especially in developmentally acyanogenic acdc1 line, was also investigated. The data on plant growth have shown that the lack of dhurrin is disadvantageous to growth, but only at the early developmental stage. The tcd1 plants also took longer to mature probably due to delayed flowering. None of the tested hormones were able to recover the wildtype phenotype. We conclude that the generation of dhurrin is advantageous for plant growth, especially at critical growth stages like germinating seed by providing a ready source of reduced nitrogen.

show abstract

Section: Resultsmentioning

confidence: 99%

Allocation of Resources to Cyanogenic Glucosides Does Not Incur a Growth Sacrifice in Sorghum bicolor (L.) Moench

Sohail

Blomstedt

Gleadow

2020

Plants

Self Cite

View full text Add to dashboard Cite

show abstract

“…Nitrate concentrations in plants are dependent on the genotype, tissue type, and water availability (Rosati et al 2019a ). Our results show that the nitrate concentration in leaves was higher in domesticated sorghum than the wild sorghum plants.…”

Section: Discussionmentioning

confidence: 99%

Transcript profiles of wild and domesticated sorghum under water-stressed conditions and the differential impact on dhurrin metabolism

Ananda

Norton

Blomstedt

et al. 2022

Planta

Self Cite

View full text Add to dashboard Cite

Main conclusion Australian native species of sorghum contain negligible amounts of dhurrin in their leaves and the cyanogenesis process is regulated differently under water-stress in comparison to domesticated sorghum species. Abstract Cyanogenesis in forage sorghum is a major concern in agriculture as the leaves of domesticated sorghum are potentially toxic to livestock, especially at times of drought which induces increased production of the cyanogenic glucoside dhurrin. The wild sorghum species endemic to Australia have a negligible content of dhurrin in the above ground tissues and thus represent a potential resource for key agricultural traits like low toxicity. In this study we investigated the differential expression of cyanogenesis related genes in the leaf tissue of the domesticated species Sorghum bicolor and the Australian native wild species Sorghum macrospermum grown in glasshouse-controlled water-stress conditions using RNA-Seq analysis to analyse gene expression. The study identified genes, including those in the cyanogenesis pathway, that were differentially regulated in response to water-stress in domesticated and wild sorghum. In the domesticated sorghum, dhurrin content was significantly higher compared to that in the wild sorghum and increased with stress and decreased with age whereas in wild sorghum the dhurrin content remained negligible. The key genes in dhurrin biosynthesis, CYP79A1, CYP71E1 and UGT85B1, were shown to be highly expressed in S. bicolor. DHR and HNL encoding the dhurrinase and α-hydroxynitrilase catalysing bio-activation of dhurrin were also highly expressed in S. bicolor. Analysis of the differences in expression of cyanogenesis related genes between domesticated and wild sorghum species may allow the use of these genetic resources to produce more acyanogenic varieties in the future.

show abstract

“…Currently, the technique is used to understand the population epigenetic diversity of living organisms. For example there is clear Methylation diversity of barley (Chwialkowska et al, 2019), Arabidopsis (Kawakatsu et al, 2016) rice (Wang et al, 2016), sorghum (Rosati et al, 2019), wheat (Shaked et al, 2001), almond (Fresnedo-Ramírez et al, 2017), grape (Fournier-Level et al, 2011), tomato (Fray and Zhong, 2015). This analysis clarified that epigenetic is important in population differentiation and influencing morphological changes.…”

Section: Introductionmentioning

confidence: 88%

Variable DNA methylation in Ensete (Ensete ventricosum) clones associated with developmental stage revealed by Amplified Fragment Length Polymorphisms (AFLPs) with Methylation-Sensitive enzyme

Degu¹,

Tesfaye²

2019

Afr. J. Biotechnol.

View full text Add to dashboard Cite

Ensete (Ensete ventricosum) is an important perennial species in Ethiopia. It is used for food and fiber. The mode of propagation is asexual and sexual. Asexual form of reproduction is by natural and anthropogenic induced sucker. This research aims to investigate and measure the methylation diversity of natural and anthropogenic induced sucker forming Ensete. Twenty-seven individuals from Hawassa University research collection were used to identify using Amplified Fragment Length Polymorphisms (AFLPs) and Methylation-Sensitive Amplification Length Polymorphism (MS-AFLP).technology. The average values of Nei's genetic diversity (Hj) were 0.189 and 0.110 for natural sucker forming and anthropogenic induced sucker forming, respectively by AFLPs analysis. With MS-AFLP analysis, this value reduced to 0.145 for natural sucker forming Ensete but increased to 0.172 for anthropogenic sucker inducing populations. The AFLP Shannon information index (Ho) was 0.313 and 0.162 for natural and anthropogenic sucker forming Ensete, respectively. However, this value was reduced to 0.238 for natural sucker forming and 0.252 for anthropogenic sucker formed by the MS-AFLP analysis. UPGMA tree, structure analysis and principal coordinate analysis (PCoA) showed the two populations clustered separately. AMOVA revealed 24% of the genetic differentiation (Fst) occurred among populations. Gene flow (Nm) was limited among all populations. This concludes that AFLP did not show differentiation among populations; however MS-AFLP clearly showed the differentiation of populations which is an indication of epigenetic diversity but limited to developmental stages of Ensete.

show abstract

Investigation into the role of DNA methylation in cyanogenesis in sorghum (Sorghum bicolor L. Moench)

Cited by 11 publications

References 49 publications

Allocation of Resources to Cyanogenic Glucosides Does Not Incur a Growth Sacrifice in Sorghum bicolor (L.) Moench

Allocation of Resources to Cyanogenic Glucosides Does Not Incur a Growth Sacrifice in Sorghum bicolor (L.) Moench

Transcript profiles of wild and domesticated sorghum under water-stressed conditions and the differential impact on dhurrin metabolism

Variable DNA methylation in Ensete (Ensete ventricosum) clones associated with developmental stage revealed by Amplified Fragment Length Polymorphisms (AFLPs) with Methylation-Sensitive enzyme

Contact Info

Product

Resources

About