Investigation into the effect that probe immobilisation method type has on the analytical signal of an EIS DNA biosensor

Lillis, Brian J.; Manning, Mary; Hurley, Eileen; Berney, Helen; Duane, Russell; Mathewson, A.; Sheehan, Michelle M.

doi:10.1016/j.bios.2006.05.021

Cited by 25 publications

(4 citation statements)

References 25 publications

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“…Table 2E presents performances of some label-free DNA biosensors or microarrays. 75,101,102,134,136,[366][367][368][369][370][371][372][373][374][375][376][377] DNA detection can be based on the natural electroactivity of the nucleotide residues present in DNA. This has been exploited in particular by Palecek group who showed that DNA and RNA are electroactive compounds producing reduction and oxidation signals after hybridization.…”

Section: Label-free Electrochemical Detectionmentioning

confidence: 99%

DNA Biosensors and Microarrays

2007

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Section: Label-free Electrochemical Detectionmentioning

confidence: 99%

DNA Biosensors and Microarrays

2007

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“…Direct functionalization by covalent attachment of a self-assembled monolayer of DNA probes to the sensor surface (either gold or oxide based) is most commonly used [11,16,17,71,72]. Nonetheless, electrostatic immobilization of probe DNA has also been described [15,22,73,74].…”

Section: Applications Of Field Effect Biosensorsmentioning

confidence: 99%

Field Effect Sensors for Nucleic Acid Detection: Recent Advances and Future Perspectives

Veigas

Fortunato

Baptista

2015

Sensors

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In the last decade the use of field-effect-based devices has become a basic structural element in a new generation of biosensors that allow label-free DNA analysis. In particular, ion sensitive field effect transistors (FET) are the basis for the development of radical new approaches for the specific detection and characterization of DNA due to FETs’ greater signal-to-noise ratio, fast measurement capabilities, and possibility to be included in portable instrumentation. Reliable molecular characterization of DNA and/or RNA is vital for disease diagnostics and to follow up alterations in gene expression profiles. FET biosensors may become a relevant tool for molecular diagnostics and at point-of-care. The development of these devices and strategies should be carefully designed, as biomolecular recognition and detection events must occur within the Debye length. This limitation is sometimes considered to be fundamental for FET devices and considerable efforts have been made to develop better architectures. Herein we review the use of field effect sensors for nucleic acid detection strategies—from production and functionalization to integration in molecular diagnostics platforms, with special focus on those that have made their way into the diagnostics lab.

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“…Integration of the sensor in a fluidic set-up allows differential measurements of several sensors simultaneously. 24 While Faradaic impedance measurements, or electrochemical impedance spectroscopy (EIS), with a macroscopic electrode are done in the presence of a redox probe, non-Faradaic impedance spectroscopy is performed in the absence of a redox probe. The combination of (non-Faradaic) impedance spectroscopy with interdigitated micro-electrode arrays 25,26 offers a sensitive label-free biosensor able to detect DNA oligonucleotides.…”

Section: Introductionmentioning

confidence: 99%

Label-free detection of DNA with interdigitated micro-electrodes in a fluidic cell

Berdat

Rodríguez²,

Herrera

et al. 2008

Lab Chip

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We investigate the analytical performance of an interdigitated electrode sensor for the label-free detection of DNA, by monitoring the complex impedance of 5 microm wide interdigitated Pt microelectrodes on a glass substrate. We detect the hybridization of unlabeled 38-mer target ssDNA with a complementary probe that is bound on the glass in between the electrodes by a disuccinimidyl terephtalate and aminosilane immobilization procedure. The sensor is mounted in a microfluidic flow cell, in which hybridization is monitored and in situ compared with a reference. After hybridization, the cell is perfused with deionised water and the dependence of the measured conductance due to the immobilized target DNA layer, to target DNA concentrations down to 1 nM is demonstrated. Subsequently, we apply our sensor to the detection of pathogen DNA from Salmonella choleraesuis in dairy food.

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Investigation into the effect that probe immobilisation method type has on the analytical signal of an EIS DNA biosensor

Cited by 25 publications

References 25 publications

DNA Biosensors and Microarrays

DNA Biosensors and Microarrays

Field Effect Sensors for Nucleic Acid Detection: Recent Advances and Future Perspectives

Label-free detection of DNA with interdigitated micro-electrodes in a fluidic cell

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