Investigation into Iron Chelating and Antioxidant Potential of Melilotus officinalis in Iron Dextran Induced Iron Overloaded Sprague Dawley Rat Model

Abstract

Excess of iron leads to generates free radicals, causes organ damage. (Fabaceae) reported to have various pharmacological activities. It contains flavonoids and phenolic compounds which have iron chelating and antioxidant property. Hence, present study was designed to investigate the beneficial effects of different fractions of for the management of iron overload disease and its complications. Iron overload was induced by 6 IP injections of iron dextran (12.5 mg/100 g) uniformly distributed over the period of 30 days. The different fractions of were given orally and Deferoxamine (DFO) subcutaneously for 30 days. The iron chelating and various biochemical parameters were estimated on 15 and 30 day. The different fractions of demonstrated dose dependant in-vitro iron chelating ability. There were significant (P<0.01) iron chelating potential shows in rats treated with methanolic fraction of methanolic extract (MFME) and methanolic fraction of aqueous extract (MFAE) of as compared to disease control (DC) rats. The rats treated with MFME and MFAE of shows significant (P<0.01) antioxidant and vital organ protective effect as compared to DC rats. Better iron chelation was observed on 30 day and at higher dose (300 mg/kg) as compared to 15 day and at lower dose (150 mg/kg). The present study concludes that MFME and MFAE of have reversible iron chelating and antioxidant potential in rats. The study also proves the possible mechanism of action, as an iron chelator by increasing the excretion of iron in urine and feces.

Trace Elements and Their Effects on Human Health and Diseases

“…We know that selenium is generally absorbed in the body in the form of L-selenomethionine. Earlier studies confirmed that in MCF-7 breast cancer, the therapeutic effect of methyl selenocysteine combined with tamoxifen and imidoselenocarbamate considered as an antitumour agent reduces the growth of breast cancer [78,79]. Combs et al [80] in their research article suggested that selenium works as anti-oxidant effects via Glutathione peroxidise (GSH-Px) which further protects the body from damaging effect of free radicals.…”

Diatom Chaetoceros Sp. as an Efficient Biological Antidote in Iron Toxicity: In‐Vitro and In‐Vivo Experiments

“…So, an investigation to find an active and effective iron chelator is essential to treat iron over‐dose disorders. Several studies have been devoted to the administration of isolated components from herbal substances including green tea, [28] Danshen, [29] Epilobium hirsutum , [30] Caesalpinia crista , [31] Melilotus officinalis [32] to treat iron overload in in vivo models.…”