Microbial Fuel Cells (MFC) are bioelectrochemical devices capable of producing direct electrical energy from electrogenic microorganisms as catalysts toward a substrate oxidation reaction. Currently, the MFC research is focused in substrates that are normally passive environmental as wastewater, which are treated and simultaneously with power generation. In this context, the dairy wastewater has been studied for MFC substrates because this wastewater has a favorable composition, as amount of biodegradable organic matter, high ionic conductivity and significant nutrient concentrations. In order to diffuse MFC as environmental technology for dairy wastewater treatment, there are a lack of technological knowledge toward to improve energy production performance and studies that address the issues of inorganic pollutants and toxicity removal. For this reason, the present thesis was developed with the construction of four different architectures of MFCs to produce direct electrical energy with the removal of pollutants from the dairy effluent. The air-cathode MFCs were constructed from acrylic as tubular with internal circulation (TCI), tubular biofilter (TB), tubular hybrid upflow (THA) and scaled cubic upflow (CA) bioreactor. The systems were lead in semi-continuous feeding mode with long-term operation using the startup consortium of Shewanella oneidenisis and Clostridium butyricum electrogenic bacteria. The MFCs produced the maximum potential of 649 mV, 540 mV, 616 mV and 583 mV, which resulted in high maximum power densities of 1.3 W/m 3 , 1.1 W/m 3 , 0.5 W/m 3 and 3.5 W/m 3 for TCI, TB, CA and THA, respectively. Notably, the hybrid tubular showed the best performance in the electricity generation. In terms of treatment, MFCs showed high COD and BOD removal with values above 90%, but only the MFC-TCI had a low COD and BOD perfomance with 62% for COD and 46% for BOD. In terms of, the nitrogen and phosphorus removal, the MFC-TB had the best performance with 85% and 92%, respectively, but the MFC-THA also presented significant values for nitrogen and phosphorus as 43% and 95%, respectively. Acute toxicity studies were applied for MFCs TB, CA and THA, in which non acute toxic effect was exhibited in dairy wastewater treated. The chronic toxicity assays were tested only to MFC-THA, which revealed chronic effect for treated effluent concentration in 40%. MFCs also showed high efficiency for nitrate and sulfate removal. Hybrid upflow MFC demonstrated exceptional results that should be explored at larger scalar, in this case electrochemical impedance spectroscopy also showed changes in the anode caused by the exchange of external resistance that is associated with high performance.