Investigation and quantification of key periodontal pathogens in patients with type 2 diabetes

Field, C. A.; Gidley, Mark D.; Preshaw, Philip M.; Jakubovics, Nicholas S.

doi:10.1111/j.1600-0765.2011.01455.x

Cited by 63 publications

(73 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Actually, both groups showed moderate to high frequency of all bacterial species, even for the pathogen A. actinomycetemcomitans, showing and approximate overall range from 40 to 90%. This observed frequency was compatible to other studies that evaluated chronic periodontitis subjects with no microbiological differences between case and control groups 14,28 . It seems that subgingival bacterial frequency was more dependent on local periodontal clinical status than on systemic diabetes status.…”

Section: Discussionsupporting

confidence: 91%

“…Although Gram-negative bacteria are less glucolytic some species are able to uptake and use glucose as a substrate 29 . Despite the fact that other researchers also failed to report microbial differences between diabetics and non-diabetics 14,28 , the present study design offered a better support for this lack of difference. Our findings can partially justify why antibiotics or antiseptics only offer diabetic subjects the same degree of benefits observed in systemic healthy controls.…”

Section: Discussioncontrasting

confidence: 66%

“…Although several studies have compared the composition of subgingival biofilm between diabetic and non-diabetic patients many aspects are still unclear [13][14][15] . Up to now literature is not enough to sustain whether a periodontal microbial difference exists or not.…”

Section: Discussionmentioning

confidence: 99%

“…However, microbial results are still controversial. According to Field et al 14 , Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans), Fusobacterium nucleatum (F. nucleatum) and Porphyromonas gingivalis (P. gingivalis) counts did not differ significantly between diabetics and non-diabetics. On the contrary, Sardi et al 15 reported that diabetics had a higher prevalence of Candida spp.…”

Section: Introductionmentioning

confidence: 95%

See 3 more Smart Citations

Salivary and microbiological parameters of chronic periodontitis subjects with and without type 2 diabetes mellitus: a case-control study

Cortelli

Pinheiro

Costa

et al. 2014

Rev. odontol. UNESP

View full text Add to dashboard Cite

ResumoIntrodução: Diversos estudos têm investigado as diferenças dos parâmetros salivares e microbianos entre pacientes diabéticos e não diabéticos, contudo, diferenças específicas ainda não estão claras, principalmente devido aos efeitos de variáveis de confusão. Objetivo: O objetivo deste estudo caso-controle foi avaliar os parâmetros salivares e microbianos de indivíduos com doença periodontal crônica com ou sem diagnóstico de diabetes melito tipo 2. Material e método: Este estudo caso-controle incluiu 60 indivíduos com periodontite crônica, 30 diabéticos (casos) e 30 não diabéticos (controles), pareados pela severidade da doença periodontal, gênero e idade. Saliva total estimulada foi coletada de todos os voluntários para mensuração do pH salivar e fluxo salivar. Amostras bacterianas foram coletadas com pontas de papel absorvente dos sítios periodontais com maior profundidade de sondagem e perda de inserção clínica. A frequência de A. actinomycetemcomitans, P. intermedia, P. gingivalis, T. forsythia e C. rectus foi avaliada por PCR. Os dados foram analisados estatisticamente pelo teste t de Student, Mann -Whitney e Qui-quadrado (p < 0,05). Resultado: Diabéticos apresentaram maior nível de glicose salivar e menor fluxo salivar em comparação aos não diabéticos. P. gingivalis e T. forsythia foram os patógenos mais frequentes (p < 0,05). Frequência bacteriana não diferiu entre os casos e controles. Conclusão: A condição diabetes influenciou o fluxo e os níveis de glicose salivar. Dentro da mesma severidade da periodontite crônica, indivíduos diabéticos não mostraram maior frequência de patógenos periodontais em comparação aos seus controles.Descritores: Saliva; bactérias; periodontite crônica; diabetes mellitus. AbstractBackground: Several studies have investigated the differences in salivary parameters and microbial composition between diabetic and non-diabetic patients, however, specific differences are still not clear mainly due to the effects of confounder. Aim: The aim of this case-control study was to evaluate the salivary and microbial parameters of chronic periodontitis subjects with and without type 2 diabetes mellitus. Material and method: This case-control study included 60 chronic periodontitis subjects, 30 diabetics (case group) and 30 non-diabetics (control group), paired according to periodontitis severity, gender and age. Stimulated whole saliva was collected from all volunteers to measure the salivary pH and the salivary flow rate. Bacterial samples were collected with paper points from periodontal sites showing the deepest periodontal pocket depth associated with the highest clinical attachment loss. The frequency of A. actinomycetemcomitans, P. intermedia, P. gingivalis, T. forsythia and C. rectus was evaluated by PCR. Data was statistically analyzed by Student's t, Mann-Whitney and Chi-square (p<0.05). Result: Diabetic subjects showed higher salivary glucose levels and lower stimulated flow rates in comparison to non-diabetic controls. P. gingivalis and T. forsythia were the most frequent pat...

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussioncontrasting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

See 2 more Smart Citations

Salivary and microbiological parameters of chronic periodontitis subjects with and without type 2 diabetes mellitus: a case-control study

Cortelli

Pinheiro

Costa

et al. 2014

Rev. odontol. UNESP

View full text Add to dashboard Cite

show abstract

“…Diabetic patients seem to be more prone to periimplantitis, in comparison with the healthy population (8,9). NIDDM patients may have -in addition to a proinflammatory phenotype -a highly pathogenic microflora, due to advanced glycosilation end product (AGE) accumulation in gingival crevicular fluid (GCF), although this fact remains controversial (10).…”

mentioning

confidence: 99%

Dental Implant Sites in Healthy versus Diabetic Subjects: A Two-Year Clinical and Bacteriological Assessment

Bignozzi

Ciobanu

Quaranta³

et al. 2013

Eur J Inflamm

View full text Add to dashboard Cite

The aim of this study was to analyze by Real-Time Polymerase Chain Reaction (PCR) possible differences in periimplant microbiota of patients without significant systemic diseases versus patients affected by non-insulin-dependent diabetes mellitus (NIDDM), both treated with dental implants with the same implant-abutment system. Patients suffering from NIDDM, and those with no history of major systemic diseases, treated with dental implants at the Prosthodontics Operative Unit of “Sapienza” University of Rome in the period February 2009 - March 2010 were considered. Clinical parameters as well as microbiological profile were evaluated for each implant site at 3, 6,12, and 24 month follow-up. Crevicular fluid was collected for microbial sampling and analyzed by Real-Time PCR in order to identify the possible presence of Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum and Prevotella intermedia. Eight patients suffering from NIDDM and 22 with no history of major systemic diseases were included in the present investigation, each having received one to three dental implants. All the implants had been loaded 3 months after surgery, and the average follow-up after implant placement was 26.37±3.86. Clinical parameters showed no noticeable difference between the two groups, except for the Implant Stability Quotient (ISQ) that showed significantly lower values in NIDDM patients. A slightly higher amount of the considered pathogenic bacteria were retrieved in samples collected from patients with NIDDM (7.38×105) in comparison with those of healthy subjects (6.78×105), though the differences were below statistical significance. Within the limitations of the present study, a slight correlation was empirically detected between gene expression profiles of microbial populations and history of NIDDM, which however remained below the statistical significance. Further well-designed clinical studies may be useful to conclusively clarify the impact of subgingival microflora on the increased susceptibility of diabetic patients to periimplantitis.

show abstract

Periodontal and microbiological data in patients with mucous membrane pemphigoid in a French population in 2021–2022: A pilot cross‐sectional study

Ejeil,

Gaultier,

Catherine

et al. 2024

Health Science Reports

View full text Add to dashboard Cite

Background and AimsIn the case of mucous membrane pemphigoid with gingival expression (gMMP), the complete healing of the gingiva is generally not achieved despite medical treatment. Therefore, patients' oral comfort is impaired. The dysbiotic periodontal microbiota, generated by a lack of oral hygiene associated with persistent gingival pain, could the immunopathological mechanism to persist. The main objective of this study was to characterize the subgingival microbiota of the gMMP patients, and to highlight a potential link between this microbiological data and the clinical data.MethodsSubgingival biofilm was collected from 15 gMMP patients, medically treated or not, but not receiving periodontal treatment. The usual clinical periodontal parameters were recorded. The biofilm was analyzed by polymerase chain reaction quantitative. The risk factors of severe erosive gingivitis and severe periodontitis were assessed using Chi‐square or Fischer's exact test were used.ResultsWhatever the medical and periodontal conditions of the patients, the results showed the existence of three main communities of periodontopathic, dysbiotic bacteria. The first including Tannnerella forsythia, Peptostreptococcus micros, Fusobacterium nucleatum, and Campylobacter rectus, was found in 100% of the patients, the second enriched with Treponema denticola in 60% and the third enriched with Porphyromonas gingivalis and Prevotella intermedia in 26%. Furthermore, there was a significant positive link between the duration of gMMP and the severity of erosive gingivitis (p = 0.009), and the loss of deep periodontal tissue (p = 0.04).ConclusionThis pilot study suggests a high periodontal risk in gMMP patients. The pathological processes, autoimmune on the one hand and plaque‐induced on the other, may amplify each other. The application of periodontal therapy is therefore necessary in parallel with medical treatment. Nevertheless, further controlled studies are required to validate and complement these preliminary results.

show abstract

Investigation and quantification of key periodontal pathogens in patients with type 2 diabetes

Cited by 63 publications

References 43 publications

Salivary and microbiological parameters of chronic periodontitis subjects with and without type 2 diabetes mellitus: a case-control study

Salivary and microbiological parameters of chronic periodontitis subjects with and without type 2 diabetes mellitus: a case-control study

Dental Implant Sites in Healthy versus Diabetic Subjects: A Two-Year Clinical and Bacteriological Assessment

Periodontal and microbiological data in patients with mucous membrane pemphigoid in a French population in 2021–2022: A pilot cross‐sectional study

Contact Info

Product

Resources

About