Investigating virus–host cell interactions: Comparative binding forces between hepatitis C virus-like particles and host cell receptors in 2D and 3D cell culture models

Collett, Simon; Torresi, Joseph; Silveira, Linda Earnest; Truong, Vi Khanh; Christiansen, Dale; Tran, Bang Manh; Vincan, Elizabeth; Ramsland, Paul A.; Elbourne, Aaron

doi:10.1016/j.jcis.2021.02.067

Cited by 17 publications

(11 citation statements)

References 59 publications

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“…Images and force curve measurements were obtained using a Cypher ES Atomic Force Microscope (Oxford Instrument, Asylum Research, Santa Barbara, CA, USA) at room temperature (25 °C). Protocols were adapted from Collett et al , For imaging, the instrument was operated in amplitude-modulated AFM (AM-AFM), while force measurements were obtained in the contact mode. Biolever BL-AC40TS cantilevers (Oxford Instruments, Asylum Research, Santa Barbara, CA, USA, nominal spring constant k c = 0.09 N/m) were used for all measurements.…”

Section: Materials and Methodsmentioning

confidence: 99%

Characterizing the Dynamic Disassembly/Reassembly Mechanisms of Encapsulin Protein Nanocages

et al. 2021

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Encapsulins, self-assembling icosahedral protein nanocages derived from prokaryotes, represent a versatile set of tools for nanobiotechnology. However, a comprehensive understanding of the mechanisms underlying encapsulin self-assembly, disassembly, and reassembly is lacking. Here, we characterize the disassembly/reassembly properties of three encapsulin nanocages that possess different structural architectures: T = 1 (24 nm), T = 3 (32 nm), and T = 4 (42 nm). Using spectroscopic techniques and electron microscopy, encapsulin architectures were found to exhibit varying sensitivities to the denaturant guanidine hydrochloride (GuHCl), extreme pH, and elevated temperature. While all three encapsulins showed the capacity to reassemble following GuHCl-induced disassembly (within 75 min), only the smallest T = 1 nanocage reassembled after disassembly in basic pH (within 15 min). Furthermore, atomic force microscopy revealed that all encapsulins showed a significant loss of structural integrity after undergoing sequential disassembly/reassembly steps. These findings provide insights into encapsulins’ disassembly/reassembly dynamics, thus informing their future design, modification, and application.

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Section: Materials and Methodsmentioning

confidence: 99%

Characterizing the Dynamic Disassembly/Reassembly Mechanisms of Encapsulin Protein Nanocages

et al. 2021

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Section: Methodsmentioning

confidence: 99%

“…Furthermore, Tm-Enc was shown to spontaneously reassemble upon returning to the initial conditions (i.e., neutral pH or absence of denaturant). 27 Interestingly, Tm-Enc can be reassembled in the presence of ESig-tagged cargo (e.g., proteins, nanomaterials), resulting in their selective encapsulation in vitro , and thus further expanding encapsulins’ utility. 8, 27, 28 29 Despite these promising findings, key questions concerning the biophysical mechanisms and physicochemical factors that underlie encapsulin disassembly/reassembly, and how they might be controlled, remain unanswered, especially for the T = 3 and T = 4 nanocages.…”

Section: Introductionmentioning

confidence: 99%

Exploring the Self-Assembly of Encapsulin Protein Nanocages from Different Structural Classes

Boyton

Goodchild

Diaz

et al. 2021

Preprint

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Encapsulins, self-assembling icosahedral protein nanocages derived from prokaryotes, represent a versatile set of tools for nanobiotechnology. However, a comprehensive understanding of the mechanisms underlying encapsulin self-assembly, disassembly, and reassembly is lacking. Here, we characterise the disassembly/reassembly properties of three encapsulin nanocages that possess different structural architectures: T = 1 (24 nm), T = 3 (32 nm), and T = 4 (42 nm). Using spectroscopic techniques and electron microscopy, encapsulin architectures were found to exhibit varying sensitivities to the denaturant guanidine hydrochloride (GuHCl), extreme pH, and elevated temperature. While all encapsulins showed the capacity to reassemble following GuHCl-induced disassembly (within 75 min), only the smallest T = 1 nanocage reassembled after disassembly in basic pH (within 15 min). Furthermore, atomic force microscopy revealed that all encapsulins show a significant loss of structural integrity after undergoing sequential disassembly/reassembly steps. These findings provide insights into encapsulins disassembly/reassembly dynamics, thus informing their future design, modification, and application.

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“…Furthermore, liver organoids can be used for the study of hepatocarcinogenesis based on HBV-related liver injury [ 81 , 82 ]. While, the researches using 3D cell culture systems to model HCV infection were more focused on HCV entry and revealed novel findings which were not seen in 2D cell lines [ 83 , 84 ]. A recent study successful recapitulated the hepatitis E virus (HEV) infection in liver organoids and further found two potent HEV inhibitors [ 85 ].…”

Section: Exploring Liver Injury Using Organoidsmentioning

confidence: 99%

Liver Organoids, Novel and Promising Modalities for Exploring and Repairing Liver Injury

Lin

et al. 2022

Stem Cell Rev and Rep

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The past decades have witnessed great advances in organoid technology. Liver is the biggest solid organ, performing multifaceted physiological functions. Nowadays, liver organoids have been applied in many fields including pharmaceutical research, precision medicine and disease models. Compared to traditional 2-dimensional cell line cultures and animal models, liver organoids showed the unique advantages. More importantly, liver organoids can well model the features of the liver and tend to be novel and promising modalities for exploring liver injury, thus finding potential treatment targets and repairing liver injury. In this review, we reviewed the history of the development of liver organoids and summarized the application of liver organoids and recent studies using organoids to explore and further repair the liver injury. These novel modalities could provide new insights about the process of liver injury. Graphical abstract

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Investigating virus–host cell interactions: Comparative binding forces between hepatitis C virus-like particles and host cell receptors in 2D and 3D cell culture models

Cited by 17 publications

References 59 publications

Characterizing the Dynamic Disassembly/Reassembly Mechanisms of Encapsulin Protein Nanocages

Characterizing the Dynamic Disassembly/Reassembly Mechanisms of Encapsulin Protein Nanocages

Exploring the Self-Assembly of Encapsulin Protein Nanocages from Different Structural Classes

Liver Organoids, Novel and Promising Modalities for Exploring and Repairing Liver Injury

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