Investigating the suitability of the Calgary Biofilm Device for assessing the antimicrobial efficacy of new agents

Ali, Laila; Khambaty, F. M.; Diachenko, Gregory W.

doi:10.1016/j.biortech.2005.08.025

Cited by 51 publications

(33 citation statements)

References 12 publications

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“…However, potency in terms of MIC values in the treatment of chronic and device-related infections due to bacterial biofilms are often found to be low (Costerton et al, 1995). Many studies have shown that biofilm-grown microorganisms have an inherent lack of susceptibility to antimicrobial agents as compared to planktonic cultures of the same organism (Costerton et al, 1995(Costerton et al, , 1999Wilson, 1996;Ceri et al, 1999;Ali et al, 2006). However, this resistance is lost once conditions are reverted to allow planktonic growth (Costerton et al, 1995).…”

Section: Discussionmentioning

confidence: 96%

“…Biofilms were formed on the lid of the device during incubation for 24 h at 37 o C and 48 hat 35 o C for bacteria and fungi, respectively. At the same conditions and inoculum, a modified microtitre plate test was applied to all strains and biofilm formation was confirmed (Abdi- Ali et al, 2006). After incubation, the peg lid was transferred to a 96-well plate in which dilutions of the antibacterial agents and the compounds were prepared in tryptic soy broth (TSB; Merck) and dilutions of amphothericin-B and the compounds were prepared in RPMI-1640 medium with L-glutamine buffered to pH 7 with MOPS.…”

Section: Microbiologymentioning

confidence: 99%

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Synthesis, antioxidant and antimicrobial evaluation of simple aromatic esters of ferulic acid

et al. 2011

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Aromatic ester derivatives of ferulic acid where the phenolic hydroxyl is free (6a-d) or acetylated (5a-d) were evaluated for their antioxidant and antimicrobial properties. The superoxide radical scavenging capacity of compounds 5d and 6d-e (IC(50) of 0.19, 0.27 and 0.20 mM, respectively) was found to be twice as active as α-tocopherol (IC(50) = 0.51 mM). DPPH radical scavenging capacity was moderate and only found in compounds bearing free phenolic hydroxyl groups (6a-e). With regard to antimicrobial properties, compounds 6b and 6c displayed significant activity against Enterococcus faecalis (MICs = 16 μg/mL) and vancomycin-resistant E. faecalis (MIC for 6b, 32 and for 6c, 16 μg/mL). Compound 6c also demonstrated prominent activity against planktonic Staphylococcus aureus with a MIC value of <8 μg/mL and it inhibited bacterial biofilm formation by S. aureus with a MBEC value of <8 μg/mL, which was 64 and 128 times more potent than ofloxacin and vancomycin, respectively.

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Section: Discussionmentioning

confidence: 96%

Section: Microbiologymentioning

confidence: 99%

Synthesis, antioxidant and antimicrobial evaluation of simple aromatic esters of ferulic acid

et al. 2011

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“…To study the efficacy of disinfectant biofilms strains, many researchers have used the Calgary Biofilms Device, MBEC TM assay, as it can support reproducible biofilms for liquid media (96). Most researchers also evaluate biofilms formation by standard plate count methods.…”

Section: Biofilmsmentioning

confidence: 99%

A review of microbial biofilms of produce: Future challenge to food safety

Jahid

2012

Food Sci Biotechnol

132

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Outbreaks of produce-related food-borne pathogens have undergone a sharp increase in last three decades because of high produce consumption. A paradigm of food safety for produce is important due to its susceptibility to microbial attack and biofilms formation. Greater attention should be paid to decontaminating the pathogens in biofilms as they pose a risk to public health. This review will focus on produce-related outbreaks, attachments, quorum sensing, biofilms formation, resistance to sanitizers and disinfectants, and current and emerging control strategies for fresh and minimally processed produce, providing new insight into food safety. The consequences of biofilms formation on produce include the formation of a protective environment that is resistant to cleaning and disinfection. Alternative means of controlling or inhibiting biofilms formation on produce will be explained briefly and we will identify where additional research is needed.

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“…After the incubation period, the lid is transferred to another 96-well plate containing antimicrobials in fresh culture media at various concentrations. The MBEC is defined as the concentration of antimicrobial in which no visible growth can be detected [88]. ASTM 2799 standard describes the operational parameters required to grow, treat, sample, and analyze Pseudomonas aeruginosa biofilms using MBEC assay.…”

Section: Methods To Study Antimicrobial Susceptibility Of Biofilmsmentioning

confidence: 99%

“…The assay is used to measure nonspecific esterase activity of viable microbial cells that converts colorless, nonfluorescent FDA to fluorescein, which is a green fluorescent compound that can be quantified fluorometrically [88]. The assay is not widely used because the dye rapidly leaks from the cells and is unstable.…”

Section: Fluorescein Diacetate (Fda) Assaymentioning

confidence: 99%

Current Approaches for Exploration of Nanoparticles as Antibacterial Agents

Karaman¹,

Manner²,

Fallarero³

et al. 2017

Antibacterial Agents

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The ascending anxiety regarding antimicrobial resistance as well as the recalcitrant nature of biofilm-associated infections call for the development of alternative strategies to treat bacterial diseases. Nanoparticles have been considered as one of the emerging and promising platforms in this respect. Their unique physical and chemical properties may lead to fine-tuned interactions between them and bacteria. In this chapter, we aim to provide an overview on the use of nanoparticles as antimicrobial agents. Both antibacterial and anti-biofilm activities of nanoparticles and their current approaches will be reviewed. The in vitro methods that are used to evaluate the potency of nanoparticles as antimicrobial agents will be discussed in detail.

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Investigating the suitability of the Calgary Biofilm Device for assessing the antimicrobial efficacy of new agents

Cited by 51 publications

References 12 publications

Synthesis, antioxidant and antimicrobial evaluation of simple aromatic esters of ferulic acid

Synthesis, antioxidant and antimicrobial evaluation of simple aromatic esters of ferulic acid

A review of microbial biofilms of produce: Future challenge to food safety

Current Approaches for Exploration of Nanoparticles as Antibacterial Agents

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