Investigating the relationship between extended spectrum β-lactamase producing Escherichia coli in the environment and food chains with the presence of this infection in people suspected of septicemia: Using the fuzzy set qualitative comparative analysis

Eshrati, Babak; Baradaran, Hamid Reza; Motevalian, Seyed Abbas; Majidpour, Ali; Boustanshenas, Mina; Moghadam, Somayeh Soleymanzadeh; Moradi, Yousef

doi:10.1007/s40201-020-00567-9

Cited by 3 publications

(2 citation statements)

References 35 publications

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“…Each homogenized sample was enriched in 9 ml of Luria Bertani (LB) broth (Invitrogen 12795027, USA) and Rappaport Vassiliadis (RV) broth (Solus RVS001, UK), both broths supplemented with cefotaxime (Caisson C032-100G, USA) as 4 mg/L of the broth and incubated at 37°C for 18 hours. One loopful from LB and RV broth was streaked directly on Mac-Conkey agar (Oxoid CM0007, UK) supplemented with cefotaxime as 4 mg/L (Mac-Cef) (Eshrati et al 2020) as well as Xylose Lysine Deoxycholate (XLD; Oxoid CM0469, UK) agar supplemented with cefotaxime (4 mg/L) (XLD-Cef), respectively. The colonies of S. enterica and E. coli were identified by their typical colony characteristics and confirmed by biochemical tests including Gram staining, Catalase test, Oxidase test, Triple Sugar Iron Agar test (TSIAT), Indole test, Methyl red test, Voges Proskauer test and Citrate utilization test (Effendi et al 2020).…”

Section: Isolation and Phenotypic Identification Of Esbl S Enterica A...mentioning

confidence: 99%

Incidence and molecular characterization of extended-spectrum beta-lactamase (ESBL)-producing Salmonella enterica and Escherichia coli of avifauna origin in Pakistan

Saeed

Waheed

Ehtisham-ul-Haque

et al. 2023

Polish Journal of Veterinary Sciences

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Members of Enterobacteriaceae are known to produce extended-spectrum beta-lactamases (ESBL) which hydrolyze the beta-lactam group of antibiotics. The existence of ESBL-producing Salmonella enterica (S. enterica) and Escherichia coli (E. coli) harbored by urban avifauna was investigated in this study. Dropping samples (n= 180) were collected from six different bird species in the district Jhang, Punjab province, Pakistan. Isolation and identification of ESBL isolates were made by using cefotaxime-(4 mg/L) supplemented MacConkey agar and double disc synergy test (DDST). Polymerase chain reaction (PCR) was performed for the detection of four different ESBL genes including bla CTX-M , bla TEM , bla SHV and bla OXA . A total of 42.69% isolates were confirmed as ESBL via DDST including 30.64% S. enterica and 49.54% E. coli. The incidence of ESBL S. enterica and ESBL E. coli was found highest in egret (Ardea alba) and pigeon (Columba livia) as 64.28% and 78.95%, respectively. The bla CTX-M gene was detected in 57.89% and 64.81% of isolates of S. enterica and E. coli, respectively. Among other genes in S. enterica and E. coli, bla TEM (21.05%, 20.4%); bla SHV (15.78%, 9.26%), and bla OXA (5.26%, 5.56%) were detected, respectively. All of the tested isolates were found resistant to at least one of the thirteen antimicrobial agents except meropenem. To the best of our knowledge, this is the first study reporting the incidence and genetic diversity of ESBL bacteria associated with urban avifauna in Pakistan. The urban avifauna can serve as a potential subject of bio-surveillance to monitor the emergence of antimicrobial-resistant bacteria.

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Section: Isolation and Phenotypic Identification Of Esbl S Enterica A...mentioning

confidence: 99%

Incidence and molecular characterization of extended-spectrum beta-lactamase (ESBL)-producing Salmonella enterica and Escherichia coli of avifauna origin in Pakistan

Saeed

Waheed

Ehtisham-ul-Haque

et al. 2023

Polish Journal of Veterinary Sciences

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“…The samples were enriched by inoculating 500 µL of the buffered peptone water containing the cloacal swab in 10 mL of Luria Bertani (LB) broth (Invitrogen, Fisherscientific, Leicestershire, UK) supplemented with cefotaxime (4 mg/L) (Oxoid, UK) and incubated at 37 • C for 24 h. A loopful of LB broth was streaked directly onto MacConkey agar (Oxoid, Hampshire, UK) and supplemented with 4 mg/L cefotaxime as previously described [46]. Based on colony morphology, typical discrete colonies were further subcultured in nutrient broth to obtain a pure culture.…”

Section: Isolation and Identification Of Esbl Producing E Colimentioning

confidence: 99%

Cross-Sectional Study for Detection and Risk Factor Analysis of ESBL-Producing Avian Pathogenic Escherichia coli Associated with Backyard Chickens in Pakistan

et al. 2023

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The increasing incidence of extended-spectrum β-lactamase (ESBL)-producing Escherichia (E.) coli in backyard chicken farming in Pakistan is of serious concern. This study aimed to assess the prevalence, antimicrobial resistance patterns and risk factors associated with ESBL avian pathogenic E. coli (APEC) isolated from backyard chickens in the Jhang district, Punjab, Pakistan. In total, 320 cloacal swabs were collected from four breeds of backyard chicken (Aseel, Golden, Misri and Necked Neck). ESBL E. coli were phenotypically identified using double disc synergy test (DDST) and corresponding genes were confirmed by multiplex polymerase chain reaction (mPCR). Out of the 320 samples, 164 (51.3%) were confirmed as E. coli, while 74 (45.1%) were characterized as ESBL E. coli. The frequency of isolation of ESBL E. coli was highest in Aseel chickens (35.1%). Of the 164 confirmed E. coli, 95.1%, 78.6%, 76.8%, 71.3%, 70.1%, 68.9%, 60.4% and 57.3% were resistant against tylosin, doxycycline, cefotaxime, enrofloxacin, colistin, trimethoprim/sulfamethoxazole, chloramphenicol and gentamicin, respectively. The ESBL gene types detected and their corresponding proportions were blaCTX-M (54.1 %, 40/74), blaTEM, (12.2%, 9/74) and co-existence (blaCTX-M and blaTEM) were shown in 33.8% (25/74). The blaCTX-M gene sequence showed homology to blaCTX-M-15 from clinical isolates. The mean multiple antibiotic resistance index (MARI) was found to be higher among ESBL E. coli (0.25) when compared to non-ESBL E. coli (0.17). Both free-range husbandry management system (p = 0.02, OR: 30.00, 95% CI = 1.47–611.79) and high antimicrobial usage in the last 6 months (p = 0.01, OR: 25.17, 95% CI = 1.81–348.71) were found significantly associated with isolation of ESBL-producing E. coli in the tested samples using binary logistic regression analysis. This study confirmed the potential of backyard chickens as a reservoir for ESBL E. coli in the Jhang district, Punjab, Pakistan.

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Antibiotic resistance of Escherichia coli isolated from food and clinical environment in China from 2001 to 2020

Liu,

Sun,

Sun

et al. 2024

Science of The Total Environment

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Investigating the relationship between extended spectrum β-lactamase producing Escherichia coli in the environment and food chains with the presence of this infection in people suspected of septicemia: Using the fuzzy set qualitative comparative analysis

Cited by 3 publications

References 35 publications

Incidence and molecular characterization of extended-spectrum beta-lactamase (ESBL)-producing Salmonella enterica and Escherichia coli of avifauna origin in Pakistan

Incidence and molecular characterization of extended-spectrum beta-lactamase (ESBL)-producing Salmonella enterica and Escherichia coli of avifauna origin in Pakistan

Cross-Sectional Study for Detection and Risk Factor Analysis of ESBL-Producing Avian Pathogenic Escherichia coli Associated with Backyard Chickens in Pakistan

Antibiotic resistance of Escherichia coli isolated from food and clinical environment in China from 2001 to 2020

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