Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes

Bogusiewicz, Joanna; Kupcewicz, Bogumiła; Goryńska, Paulina Zofia; Jaroch, Karol; Goryński, Krzysztof; Birski, Marcin; Furtak, Jacek; Pa̧czkowski, Dariusz; Harat, Marek; Bojko, Barbara

doi:10.20944/preprints202203.0018.v1

Cited by 3 publications

(1 citation statement)

References 56 publications

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“…Although the relative concentration obtained from the intact tissue corresponded to the concentration measured in the homogenate (calculated based on the standard addition method), several issues should be discussed prior to further attempts to develop the calibration strategy for determining endogenous compounds in cancer tissue with CBS-MS. First, since brain tumors are characterized by high heterogeneity, the homogenization of a small section may only result in the selection of a portion of the tumor's phenotype [22]. Conversely, SPME probes enable the extraction of analytes along the sorbent from various parts of the tumor [30]. Secondly, the CBS-MS device has a bigger extraction surface than SPME fibers, which means that it can be influenced more easily by the composition of a changed region (e.g., the calcified part of the tumor where extraction is altered at a specific part of the blade).…”

Section: Resultsmentioning

confidence: 99%

Coated Blade Spray-Mass Spectrometry as a New Approach for the Rapid Characterization of Brain Tumors

Bogusiewicz¹,

Gaca-Tabaszewska²,

Olszówka³

et al. 2022

Preprint

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Brain tumors are neoplasms with one of the highest mortality rates. Therefore, the availability of methods that allow the quick and effective diagnosis of brain tumors and selection of appropriate treatments is of critical importance for patient outcomes. In this study, coated blade spray-mass spectrometry (CBS-MS), which combines the features of microextraction and fast ionization methods, is applied for the analysis of brain tumors. In this approach, a sword-shaped probe is coated with a sorptive material to enable the extraction of analytes from biological samples. The analytes are then desorbed using only a few microliters of solvent, followed by the insertion of the CBS device into the interface on the mass spectrometer source. The results of this proof-of-concept experiment confirmed that CBS coupled to high-resolution mass spectrometry (HRMS) enables the rapid differentiation of two histologically different lesions: meningiomas and gliomas. Moreover, quantitative CBS-HRMS/MS analysis of carnitine, the endogenous compound, previously identified as discriminating metabolite showed good reproducibility with the variation below 10% when using a standard addition calibration strategy and deuterated internal standards for correction. The resultant data show that the proposed CBS-MS technique can be useful for on-site qualitative and quantitative assessments of brain tumor metabolite profiles.

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Section: Resultsmentioning

confidence: 99%

Coated Blade Spray-Mass Spectrometry as a New Approach for the Rapid Characterization of Brain Tumors

Bogusiewicz¹,

Gaca-Tabaszewska²,

Olszówka³

et al. 2022

Preprint

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Solid-phase microextraction: a fit-for-purpose technique in biomedical analysis

Bojko

2022

Anal Bioanal Chem

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Solid-phase microextraction (SPME) possesses unique features that allow it to be used in analyses that would not be possible with traditional sample-preparation methods. The simplicity of SPME protocols and extraction devices makes it a uniform platform for analyzing biological samples, either via the headspace or in direct immersion mode. Furthermore, flexible probe design enables SPME to be applied to target objects of different sizes, offering analysis on a scale ranging “from single cell to living organs”. SPME microfibers are minimally invasive, which enables them to be applied for the spatial and temporal monitoring of target analytes or to assess changes in the entire metabolome or lipidome. Furthermore, SPME permits the capture of the elusive portion of the metabolome, thus complementing exhaustive methods that are biased towards highly abundant and stable species. Significantly, SPME can be interfaced with analytical instrumentation to create a rapid diagnostic tool. However, despite these advantages, SPME has some limitations that must be well-understood and addressed. This paper presents examples of up-to-date applications of SPME, challenges related to particular studies, and future perspectives regarding the application of SPME in biomedical analysis.

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Biliary Metabolome Profiling for Evaluation of Liver Metabolism and Biliary Tract Function Related to Organ Preservation Method and Degree of Ischemia in a Porcine Model

Łuczykowski

Warmuzińska

Kollmann

et al. 2023

IJMS

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The development of surgical techniques, immunosuppressive strategies and new organ preservation methods have meant that transplant centers have to face the problem of an insufficient number of organs for transplantation concerning the constantly growing demand. Therefore, using organs from expanded criteria donors and developing new analytical solutions to find parameters or compounds that would allow a more efficient assessment of organ quality before transplantation are options for meeting this challenge. This study proposed bile metabolomic analysis to evaluate liver metabolism and biliary tract function depending on the organ preservation method and degree of warm ischemia time. The analyses were performed on solid-phase microextraction-prepared bile samples from porcine model donors with mild (heart beating donor [HBD]) and moderate warm ischemia (donation after circulatory death [DCD]) grafts subjected to static cold storage (SCS) or normothermic ex vivo liver perfusion (NEVLP) before transplantation. Bile produced in the SCS-preserved livers was characterized by increased levels of metabolites such as chenodeoxycholic acid, arachidonic acid and 5S-hydroxyeicosatetraeonic acid, as well as saturated and monounsaturated lysophosphatidylcholines (LPC). Such changes may be associated with differences in the bile acid synthesis pathways and organ inflammation. Moreover, it has been shown that NEVLP reduced the negative effect of ischemia on organ function. A linear relationship was observed between levels of lipids from the LPC group and the time of organ ischemia. This study identified metabolites worth considering as potential markers of changes occurring in preserved grafts.

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Investigating the Potential Use of Chemical Biopsy Devices to Characterize Brain Tumor Lipidomes

Cited by 3 publications

References 56 publications

Coated Blade Spray-Mass Spectrometry as a New Approach for the Rapid Characterization of Brain Tumors

Coated Blade Spray-Mass Spectrometry as a New Approach for the Rapid Characterization of Brain Tumors

Solid-phase microextraction: a fit-for-purpose technique in biomedical analysis

Biliary Metabolome Profiling for Evaluation of Liver Metabolism and Biliary Tract Function Related to Organ Preservation Method and Degree of Ischemia in a Porcine Model

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