Investigating the photo-thermo-radiosensitization effects of folate-conjugated gold nanorods on KB nasopharyngeal carcinoma cells

Movahedi, Mohammad Mehdi; Mehdizadeh, Alireza; Koosha, Fereshteh; Eslahi, Neda; Mahabadi, Vahid Pirhajati; Ghaznavi, Habib; Shakeri‐Zadeh, Ali

doi:10.1016/j.pdpdt.2018.10.016

Cited by 40 publications

(27 citation statements)

References 31 publications

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“…regimen (Movahedi et al, 2018). We observed that the cells treated with a combination of gold nanorods, laser and X-rays experience significant damages, such as plasma membrane blebbing and chromatin condensation as the typical morphological characteristics of apoptosis.…”

Section: Discussionmentioning

confidence: 97%

Combinatorial effects of radiofrequency hyperthermia and radiotherapy in the presence of magneto‐plasmonic nanoparticles on MCF‐7 breast cancer cells

Hadi

Tavakkol

Laurent

et al. 2019

Journal Cellular Physiology

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Here, the effects of combinatorial cancer therapy including radiotherapy (RT) and radiofrequency (RF) hyperthermia in the presence of gold-coated iron oxide nanoparticles (Au@IONPs), as a thermo-radio-sensitizer, are reported. The level of cell death and the ratio of Bax/Bcl2 genes, involved in the pathway of apoptosis, were measured to evaluate the synergistic effect of Au@IONPs-mediated RF hyperthermia and RT. MCF-7 human breast adenocarcinoma cells were treated with different concentrations of Au@IONPs. After incubation with NPs, the cells were exposed to RF waves (13.56 MHz; 100 W; 15 min). At the same time, thermometry was performed with an infrared (IR) camera. Then, the cells were exposed to 6 MV X-ray at various doses of 2 and 4 Gy. MTT (3-[4,5-dimethylthiazol-2-y1]-2, 5-diphenyltetrazolium bromide) assay was performed to evaluate cell viability and quantitative real-time polymerase chain reaction (qRT-PCR) was used to determine the expression ratio of Bax/Bcl2. Cellular uptake of nanoparticles was confirmed qualitatively and quantitatively. The results obtained from MTT assay and qRT-PCR studies showed that NPs and RF hyperthermia had no significant effect when applied separately, while their combination had synergistic effects on cell viability percentage and the level of apoptosis induction. A synergistic effect was also observed when the cancer cells were treated with a combination of NPs, RF hyperthermia, and RT. On the basis of the obtained results, it may be concluded that the use of magnetoplasmonic NPs in the process of hyperthermia and RT of cancer holds a great promise to develop a new combinatorial cancer therapy strategy. K E Y W O R D S cancer, combination therapy, magneto-plasmonic nanoparticles, radiofrequency hyperthermia, radiotherapy

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Section: Discussionmentioning

confidence: 97%

Combinatorial effects of radiofrequency hyperthermia and radiotherapy in the presence of magneto‐plasmonic nanoparticles on MCF‐7 breast cancer cells

Hadi

Tavakkol

Laurent

et al. 2019

Journal Cellular Physiology

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“…This displacement is referred to as Polarization in atomic physics. Density of electron cloud, physical parameters of electron cloud around nucleus, and the effective electron mass can change the frequency of this oscillation [12]. Considering nano-particles with scales much smaller than the wavelength of the incident electromagnetic wave, the atomic polarization characterized by the dipole mode of oscillation is the dominant factor prescribing SPR frequency.…”

Section: Introductionmentioning

confidence: 99%

Simulation and In Vitro Experimental Studies on Targeted Photothermal Therapy of Cancer using Folate-PEG-Gold Nanorods

2021

j Biomed Phys Eng

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Background: Selective targeting of malignant cells is the ultimate goal of anticancer studies around the world. There are some modalities for cancer therapy devastating tumor size and growth rate, meanwhile attacking normal cells. Utilizing appropriate ligands, like folate, allow the delivery of therapeutic molecules to cancer cells selectively. There are a variety of photosensitizers, like gold nanorods (GNRs), capable of absorbing the energy of light and converting it to heat, evidently build a photothermal procedure for cancer therapy.Objective: To develop a one-step approach for calculating the temperature distribution by solving the heat transfer equation with multiple heat sources originating from NIR laser-exposed GNRs. Material and Methods:In this experimental study, we simulated NIR laser heating process in a single cancer cell, with and without incubation with folate conjugated PEG-GNRs. This simulation was based on a real TEM image from an experiment with the same setup. An in vitro experiment based on aforesaid scenario was performed to validate the simulated model in practice.Results: According to the simplifications due to computational resource limits, the resulting outcome of simulation showed significant compatibility to the supporting experiment. Both simulation and experimental studies showed a similar trend for heating and cooling of the cells incubated with GNRs and irradiated by NIR laser (5 min, 1.8 W/cm 2 ). It was observed that temperature of the cells in microplate reached 53.6 °C when irradiated by laser. Conclusion:This new method can be of great application in developing a planning technique for treating tumors utilizing GNP-mediated thermal therapy.

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“…Therefore, irradiating nanorods in the longitudinal plasmon resonance with near-infrared (NIR) laser makes tumor cells more sensitive to photothermal damage [29][30][31][32]. Overall, AuNRs can have more cellular uptake, depending on the surface charge and type of the functional group [33][34][35][36]. Some studies have reported that both AuNPs and AuNRs can effectively sensitize tumor cells to radiation in different cancer cell lines [18].…”

Section: Introductionmentioning

confidence: 99%

Investigation of the Dose-Enhancement Effects of Spherical and Rod-Shaped Gold Nanoparticles on the HeLa Cell Line

Amani

Mehdizadeh

Movahedi

et al. 2020

GMJ

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Background: Cervical cancer cells are known as radioresistant cells. Current treatment methods have not improved the patients’ survival efficiently; thus, new therapeutic strategies are needed to enhance the efficacy of radiotherapy. Gold nanomaterials with different shapes and sizes have been explored as radiosensitizers. The present study compared the radiosensitizing effects of gold nanorods (AuNRs) with spherical gold nanoparticles (AuNPs) on the HeLa cell line irradiated with megavoltage X-rays. Materials and Methods: The cytotoxicity of AuNRs and AuNPs on HeLa cells in the presence and absence of 6-MV X-ray was investigated using the MTT assay. For this aim, HeLa cells were incubated with and AuNPs and AuNRs at various concentrations (5, 10, and 15 µg/mL) for 6 hours. Afterward, HeLa cells were irradiated with 6-MV X-ray at a single dose of 2 Gy. Results: The results showed that the addition of AuNRs and AuNPs could enhance the radiosensitivity of HeLa cells. Both AuNRs and AuNPs showed low toxicity on HeLa cells, while AuNRs were more toxic than AuNPs at the examined concentrations. Moreover, it was found that AuNRs could enhance the radiosensitivity of HeLa cells more than spherical-shaped AuNPs. Conclusion: This study revealed that the shape of nanoparticles is an effective factor when they are used as radiosensitizing agents during radiotherapy. [GMJ.2020;9:e1581]

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Investigating the photo-thermo-radiosensitization effects of folate-conjugated gold nanorods on KB nasopharyngeal carcinoma cells

Cited by 40 publications

References 31 publications

Combinatorial effects of radiofrequency hyperthermia and radiotherapy in the presence of magneto‐plasmonic nanoparticles on MCF‐7 breast cancer cells

Combinatorial effects of radiofrequency hyperthermia and radiotherapy in the presence of magneto‐plasmonic nanoparticles on MCF‐7 breast cancer cells

Simulation and In Vitro Experimental Studies on Targeted Photothermal Therapy of Cancer using Folate-PEG-Gold Nanorods

Investigation of the Dose-Enhancement Effects of Spherical and Rod-Shaped Gold Nanoparticles on the HeLa Cell Line

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