Investigating the Mechanism of Action of Schisandra chinensis Combined with Coenzyme Q10 in the Treatment of Heart Failure Based on PI3K-AKT Pathway

Abstract

To study the active components, drug targets and mechanism of Schisandra chinensis (S.chinensis) combined with coenzyme Q10 (CQ10) in the treatment of heart failure (HF). Methods: Network pharmacology combined with the gene expression omnibus chip method to analyze the main pathways by which S.chinensis combined with CQ10 functioned to treat heart failure. Subsequently, the biological activities of the major pathway key proteins and their corresponding compounds were verified by molecular docking techniques. Finally, the molecular mechanism of S. chinensis combined with CQ10 for the treatment of heart failure was verified using a rat heart failure model induced by isoproterenol hydrochloride and using hematoxylin-eosin staining, TUNEL, immunohistochemistry and Western blot. Results: Network pharmacology combined with experimental validation suggests that the mechanism of action of S.chinensis combined with CQ10 in the treatment of heart failure may involve CQ10, Citral, Schisandrone, Schisanhenol B, Gomisin O, Schisandrin C and other components, which may synergistically inhibit the PI3K-AKT signaling pathway and affect the expression of AKT1, PIK3CG and other targets on this pathway. In addition, S. chinensis combined with CQ10 could effectively improve the cardiac coefficients of rats with heart failure, reduce the area of myocardial fibrosis and lowered the serum levels of IL-1β and TNF-α in heart failure rats, as well as reduced cardiac myocyte apoptosis, increased Bcl-2 expression and decreased p-PI3K/PI3K, p-AKT/ AKT, P65 and Bax expression in cardiac tissue. Comparison of the results showed that the combination of S.chinensis and CQ10 was more effective compared with CQ10 alone, ie, the ability of S.chinensis combined with CQ10 in improving cardiac function, inhibiting cardiomyocyte apoptosis and reducing inflammatory response lies in the synergistic effect of PI3K/AKT signaling pathway. Conclusion:The therapeutic effect of S.chinensis combined with CQ10 on heart failure, which may occur through the inhibition of PI3K/AKT signaling pathway.

A Review of Extraction and Purification, Biological Properties, Structure–Activity Relationships and Future Prospects of Schisandrin C: A Major Active Constituent of Schisandra Chinensis

“…Further exploring its anti‐atherosclerotic molecular mechanism, it was hypothesized that Sch C may alleviate arterial inflammation by inhibiting the phosphorylation and degradation of IκB α [68] . Network pharmacology combined with experimental validation showed that Sch C may be one of the active components in the inhibition of heart failure, and its combination with CQ10 could effectively improve cardiac coefficients and reduce serum levels of IL‐1 β and TNF‐ α in rats with heart failure, as well as reduce apoptosis of cardiomyocytes, increase the expression of Bcl‐2, and reduce p‐AKT/AKT, P65, and Bax in heart tissue expression in heart tissue [69] …”

Сок Лимонника: Полезные Свойства И Особенности Употребления Уникального Напитка

“…Research shows that schizandra juice, especially in combination with coenzyme Q10, helps reduce the area of myocardial fibrosis in patients with heart failure, improves heart function [15] and protects against the development of cardiovascular diseases. [16]…”