Investigating the Link Between Imipenem Resistance and Biofilm Formation by Pseudomonas aeruginosa

Musafer, Hadeel Kareem; Kuchma, Sherry L.; Naimie, Amanda A.; Schwartzman, Joseph D.; Al-Mathkhury, Harith Jabbar Fahad; O’Toole, George A.

doi:10.1007/s00248-013-0361-6

Cited by 26 publications

(13 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This study found that out of 24 strains of P. aeruginosa studied, 16 were biofilm producers and most of the biofilm forming strains were resistant to gentamicin and ciprofloxacin. This strongly supports the earlier studies that there may be an unidentified mechanism between planktonic antibiotic resistance and biofilm formation because there is a need for higher concentration of antibiotics to kill biofilm cells than planktonic cells [15]. It was also noted in this study that antibiotics such as gentamicin and ciprofloxacin which are thought to be a first choice of antibiotics for Pseudomonas infections is not true anymore because both the planktonic and biofilm-forming cells showed extreme resistance to gentamicin and ciprofloxacin.…”

Section: Discussionsupporting

confidence: 90%

“…The rate of biofilm-forming multi-drug resistant P. aeruginosa is increasing in India and the developing antibiotic-resistant biofilm forming P. aeruginosa is worrisome [14]. Recent studies showed that biofilm producing P. aeruginosa isolated from clinical samples were resistant to amikacin, imipenem, ciprofloxacin and gentamicin [14][15][16]. This study found that out of 24 strains of P. aeruginosa studied, 16 were biofilm producers and most of the biofilm forming strains were resistant to gentamicin and ciprofloxacin.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Role of biofilm-specific antibiotic resistance genes PA0756-0757, PA5033 and PA2070 inPseudomonas aeruginosa

Manohar

Shanthini

et al. 2018

Preprint

View full text Add to dashboard Cite

To evaluate the presence of biofilm-specific antibiotic-resistant genes, PA0756-0757, PA5033 and PA2070 in Pseudomonas aeruginosa isolated from clinical samples in Tamil Nadu. For this cross-sectional study, 24 clinical isolates (included pus, urine, wound, and blood) were collected from two diagnostic centers in Chennai from May 2015 to February 2016. Biofilm formation was assessed using microtiter dish biofilm formation assay and minimal inhibitory concentration (MIC) and minimal bactericidal concentrations (MBC) were determined for planktonic and biofilm cells (MBC assay). Further, PCR amplification of biofilm-specific antibiotic resistance genes PA0756-0757, PA5033 and PA2070 were performed. Biofilm formation was found to be moderate/strong in 16 strains. MBC for planktonic cells showed that 4, 7, 10 and 14 strains were susceptible to gentamicin, ciprofloxacin, meropenem and colistin respectively. In MBC assay for biofilm cells (MBC-B), all the 16 biofilm producing strains were resistant to ciprofloxacin and gentamicin whereas nine and four were resistant to meropenem, and colistin respectively. The biofilm-specific antibiotic-resistant genes PA0756-0757 was found in 10 strains, 6 strains with PA5033 and 9 strains with PA2070 that were found to be resistant phenotypically. This study highlighted the importance of biofilm-specific antibiotic resistance genes PA0756-0757, PA5033, and PA2070 in biofilm-forming P. aeruginosa.

show abstract

Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Role of biofilm-specific antibiotic resistance genes PA0756-0757, PA5033 and PA2070 inPseudomonas aeruginosa

Manohar

Shanthini

et al. 2018

Preprint

View full text Add to dashboard Cite

show abstract

“…Further analysis of possible changes in membrane protein profiles, gene expression or production of reactive oxygen species could help to understand the observed differences in these strains. As a consequence, and as has been observed for other organisms (Musafer et al, 2014), in vitro analysis of K. pneumoniae biofilm formation capacity does not necessarily correlate with drug resistance -an aspect that merits more detailed studies using clinical isolates given the importance of this pathogen in HAI infections.…”

Section: Discussionmentioning

confidence: 99%

Klebsiella pneumoniae yfiRNB operon affects biofilm formation, polysaccharide production and drug susceptibility

et al. 2014

View full text Add to dashboard Cite

Klebsiella pneumoniae is an opportunistic pathogen important in hospital-acquired infections, which are complicated by the rise of drug-resistant strains and the capacity of cells to adhere to surfaces and form biofilms. In this work, we carried out an analysis of the genes in the K. pneumoniae yfiRNB operon, previously implicated in biofilm formation. The results indicated that in addition to the previously reported effect on type 3 fimbriae expression, this operon also affected biofilm formation due to changes in cellulose as part of the extracellular matrix. Deletion of yfiR resulted in enhanced biofilm formation and an altered colony phenotype indicative of cellulose overproduction when grown on solid indicator media. Extraction of polysaccharides and treatment with cellulase were consistent with the presence of cellulose in biofilms. The enhanced cellulose production did not, however, correlate with virulence as assessed using a Caenorhabditis elegans assay. In addition, cells bearing mutations in genes of the yfiRNB operon varied with respect to the WT control in terms of susceptibility to the antibiotics amikacin, ciprofloxacin, imipenem and meropenem. These results indicated that the yfiRNB operon is implicated in the production of exopolysaccharides that alter cell surface characteristics and the capacity to form biofilms -a phenotype that does not necessarily correlate with properties related with survival, such as resistance to antibiotics.

show abstract

“…Interestingly, our findings showed that carbapenemase-producing isolates formed less biofilm than ESBL-producing and non-producing isolates. Recent studies demonstrate different results, carbapenemase producers P.aureginosa and A. baumannii formed stronger biofilms than carbapenemase negative isolates [33][34][35] and an inverse relationship between imipenem-meropenem resistance and biofilm formation [36,37]. These different results can be explained by the clonality (or hetrogeneity) of the carbapenemase-producing isolates [33].…”

Section: Discussionmentioning

confidence: 99%

Comparison of biofilm formation and efflux pumps in ESBL and carbapenemase producing Klebsiella pneumoniae

Yazgan

Türkel

Güçkan

et al. 2018

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Klebsiella pneumoniae is an opportunistic pathogen that causes a range of diseases. The appearance of extended-spectrum β-lactamase -and carbapenemase-producing strains, in addition to the biofilm-forming phenotype, is a major problem in the clinical environment. Methodology: A total of 33 clinical K. pneumoniae isolates were used in this study. Antimicrobial susceptibilities were assessed by a disc diffusion assay. Biofilm formation was determined by a microtiter plate assay, staining with 1% crystal violet and measuring absorbance after destaining. Moreover, expression of acrA, kdeA, ketM, kpnEF, and kexD efflux associated genes was measured by qRT-PCR. Results: Isolates displayed high resistance to β-lactams such as cefazolin, cefuroxime, ceftriaxone, cefepime, piperacillin-tazobactam, imipenem, and meropenem and decreased resistance to gentamicin, amikacin, ciprofloxacin, and levofloxacin. ESBL-producing isolates formed more biofilm than carbapenemase-producing isolates. The mRNA expression levels in KPC isolates for acrA (2-fold), kdeA (2.7-fold), ketM (2.2-fold), and kpnEF (3.4-fold) were significantly increased compared to ESBL-producing isolates. There was no significant difference in kexD expression level. Conclusions: Under the conditions used here ESBL-producing isolates formed more biofilm than KPC postive isolates; this was associated with virulence determinants which were also transferred by plasmids together with ESBLs enzymes. Moreover, the upregulation of acrA, kdeA, ketM, and kpnEF efflux pumps was seen in carbapenemase-producing isolates demonstrating that high expression of efflux pumps alone could not confer resistance but may act as a physiological determinant such as bacterial pathogenicity and virulence, and cell-to-cell communication for bacteria.

show abstract

Investigating the Link Between Imipenem Resistance and Biofilm Formation by Pseudomonas aeruginosa

Cited by 26 publications

References 53 publications

Role of biofilm-specific antibiotic resistance genes PA0756-0757, PA5033 and PA2070 inPseudomonas aeruginosa

Role of biofilm-specific antibiotic resistance genes PA0756-0757, PA5033 and PA2070 inPseudomonas aeruginosa

Klebsiella pneumoniae yfiRNB operon affects biofilm formation, polysaccharide production and drug susceptibility

Comparison of biofilm formation and efflux pumps in ESBL and carbapenemase producing Klebsiella pneumoniae

Contact Info

Product

Resources

About