Investigating the Life Cycle of HIV with Fluorescent Proteins

Baumgärtel, Viola; Ivanchenko, Sergey; Müller, Bárbara; Lamb, Don C.

doi:10.1007/4243_2011_32

Search citation statements

Order By: Relevance

Paper Sections

Select...

Applications Of Quantitative Fluorescence Microscopy In E1

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2012

Publication Types

Select...

Article1

Relationship

Self Cite1

Independent0

Authors

Journals

Cited by 1 publication

(1 citation statement)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The majority of VPS4A activity took place during phase II of the assembly process before the actual release of the virus [ 20 ]. Figure adapted from Baumgärtel et al [ 92 ].…”

Section: Applications Of Quantitative Fluorescence Microscopy In Ementioning

confidence: 99%

Quantitative Live-Cell Imaging of Human Immunodeficiency Virus (HIV-1) Assembly

Baumgärtel

Müller

Lamb

2012

Viruses

Self Cite

View full text Add to dashboard Cite

Advances in fluorescence methodologies make it possible to investigate biological systems in unprecedented detail. Over the last few years, quantitative live-cell imaging has increasingly been used to study the dynamic interactions of viruses with cells and is expected to become even more indispensable in the future. Here, we describe different fluorescence labeling strategies that have been used to label HIV-1 for live cell imaging and the fluorescence based methods used to visualize individual aspects of virus-cell interactions. This review presents an overview of experimental methods and recent experiments that have employed quantitative microscopy in order to elucidate the dynamics of late stages in the HIV-1 replication cycle. This includes cytosolic interactions of the main structural protein, Gag, with itself and the viral RNA genome, the recruitment of Gag and RNA to the plasma membrane, virion assembly at the membrane and the recruitment of cellular proteins involved in HIV-1 release to the nascent budding site.

show abstract

“…The majority of VPS4A activity took place during phase II of the assembly process before the actual release of the virus [ 20 ]. Figure adapted from Baumgärtel et al [ 92 ].…”

Section: Applications Of Quantitative Fluorescence Microscopy In Ementioning

confidence: 99%