Investigating the Isolation and Amplification of microRNAs for Forensic Body Fluid Identification

Leary, Kelsie R O; Glynn, Claire L.

doi:10.2174/2211536607666180430153821

Cited by 21 publications

(13 citation statements)

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“…Previous published studies reported promising results identifying differentially expressed miRNAs with great potential as novel biomarkers for forensic BFID (Hanson et al 2009;Zubakov et al 2010;Courts and Madea 2011;Wang et al 2013;Sauer et al 2016;Seashols-Williams et al 2016;Sirker et al 2017;Mayes et al 2018;O'Leary and Glynn 2018;Tian et al 2018). In the small number of studies published in this area, however, there is little agreement between them, with only some miRNAs suggested as potential biomarkers for specific fluids overlapping between studies (see Table 1).…”

Section: Body Fluid Identificationmentioning

confidence: 99%

Potential applications of microRNA profiling to forensic investigations

Glynn

2019

RNA

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Within the forensic science community, there is a continued push to develop novel tools to aid in criminal investigations. microRNA (miRNA) analysis has been the focus of many researcher's attention in the biomedical field since its discovery in 1993; however, the forensic application of miRNA analysis has only been suggested within the last 10 years and has been gaining considerable traction recently. The primary focus of the forensic application of miRNA analysis has been on body fluid identification to provide confirmatory universal analysis of unknown biological stains obtained from crime scenes or evidence items. There are, however, other forensic applications of miRNA profiling that have shown potential, yet are largely understudied, and warrant further investigation such as organ tissue identification, donor age estimation, and more. This review paper aims to evaluate the current literature and future potential of miRNA analysis within the forensic science field.

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Section: Body Fluid Identificationmentioning

confidence: 99%

Potential applications of microRNA profiling to forensic investigations

Glynn

2019

RNA

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“…Other biomarkers, such as RNAs, have also been proposed and employed in forensic science [29][30][31] and, in addition, with advances in molecular biological techniques, novel approaches based on miRNA profi ling have been developed for a possible forensic application [32]. For example, the circulating miRNAs have been considered as potential tool for identifi cation of sex [33], body fl uids [34,35] or organ tissue types [36].…”

Section: Discussionmentioning

confidence: 99%

Long-term persistence of Saliva-derived microRNA in human dental calculus for Forensic investigations

Lippolis¹,

Cavallini²,

Panzarino³

et al. 2019

Forensic Sci Today

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Introduction:Human identifi cation is one of the major fi elds in forensic odontology especially when the dental remains are the only available evidence to individual identifi cation. In this context DNA analysis in teeth pulp remains the most accurate and reliable method for identifi cation, whereas RNA is less used for forensic purpose because it is generally considered a quickly degradable molecule. Recently some studies report that another excellent source of nucleic acids is the dental calculus or tartar.Method: Therefore to assess the RNA stability over time, we investigated 40 saliva-derived microRNAs (miRNAs), in archeological and modern tartar samples by classic PCR followed by quantitative PCR.Result: Three miRNAs were not present in both ancient and modern tartar samples, while 10 out of 37 miRNAs had signifi cant different levels in ancient than modern samples. Nine miRNAs were down-regulated and only one was up-regulated. Since these different levels were not induced by gum infl ammation and were independent to guanine-cytosine content in miRNA sequences, used as degradation biomarker, we hypothesized as a possible cause the different eating habits between ancient and modern populations. This hypothesis is also supported by recent studies that demonstrate an close relationship between nutrients and changes in miRNA expression in healthy individuals. Conclusion:Overall data show that miRNAs are more stable than other RNA types in ancient tartar and this could enlarge the possibility of their practical use for forensic purpose and bioarchaeological researches although to better understand the variability of some miRNA levels between ancient and modern populations requires further investigation.

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“…As most compounds present in blood can also be found in saliva, saliva is suggested to mirror the physiological state of the body [ 18 ]. In this regard, the use of saliva miRs has been extended to exercise evaluation [ 11 ] and forensic body fluid identification [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

A Traditional Korean Diet Alters the Expression of Circulating MicroRNAs Linked to Diabetes Mellitus in a Pilot Trial

et al. 2020

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The traditional Korean diet (K-diet) is considered to be healthy and circulating microRNAs (miRs) have been proposed as useful markers or targets in diet therapy. We, therefore, investigated the metabolic influence of the K-diet by evaluating the expression of plasma and salivary miRs. Ten women aged 50 to 60 years were divided into either a K-diet or control diet (a Westernized Korean diet) group. Subjects were housed in a metabolic unit-like condition during the two-week dietary intervention. Blood and saliva samples were collected before and after the intervention, and changes in circulating miRs were screened by an miR array and validated by individual RT-qPCRs. In the K-diet group, eight plasma miRs were down-regulated by array (p < 0.05), out of which two miRs linked to diabetes mellitus, hsa-miR26a-5p and hsa-miR126-3p, were validated (p < 0.05). Among five down-regulated salivary miRs, hsa-miR-92-3p and hsa-miR-122a-5p were validated, which are associated with diabetes mellitus, acute coronary syndrome and non-alcoholic fatty liver disease. In the control diet group, validated were down-regulated plasma hsa-miR-25-3p and salivary hsa-miR-31-5p, which are associated with diabetes mellitus, adipogenesis and obesity. The K-diet may influence the metabolic conditions associated with diabetes mellitus, as evidenced by changes in circulating miRs, putative biomarkers for K-diet.

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Investigating the Isolation and Amplification of microRNAs for Forensic Body Fluid Identification

Abstract: This research highlights the potential of miRNAs as novel markers for the identification of forensically relevant body fluids.

Cited by 21 publications

References 0 publications

Potential applications of microRNA profiling to forensic investigations

Potential applications of microRNA profiling to forensic investigations

Long-term persistence of Saliva-derived microRNA in human dental calculus for Forensic investigations

A Traditional Korean Diet Alters the Expression of Circulating MicroRNAs Linked to Diabetes Mellitus in a Pilot Trial

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