Investigating the gut microbiome and metabolome following treatment with artificial sweeteners acesulfame potassium and saccharin in young adult Wistar rats

Murali, Aishwarya; Giri, Varun; Cameron, Hunter James; Sperber, Saskia; Zickgraf, Franziska Maria; Haake, Volker; Driemert, Peter; Walk, T.; Kamp, Hennicke; Rietjens, I.M.C.M.; Ravenzwaay, Bennard van

doi:10.1016/j.fct.2022.113123

Cited by 9 publications

(20 citation statements)

References 35 publications

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“…This animal study was performed in an AAALAC-approved (Association for Assessment and Accreditation of Laboratory Animal Care International) laboratory in compliance with the German Animal Welfare Act and the effective European Council Directive. This study was approved by the BASF Animal Welfare Body, with the permission of the local authority, the Landesuntersuchungsamt Koblenz, Germany (approval number 23 177-07/G 18-3-098) [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…Wistar rats of both sexes (CrI:WI(Han)) of the age group 70 ± 1 days were purchased from Charles River, Germany, prior to the start of the planned study. The handling of the animals, acclimatization, conditions of the animal test facility, and other related information regarding the study have been presented and published by Murali et al 2022 [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…Standard operating procedures following the Organization for Economic Co-operation and Development (OECD) 407 guidelines were used for all clinical and pathological examinations. Details related to regular examinations have been followed based on our previous publication, Murali et al 2022 [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…Dry weights of stored fecal samples collected on days 1, 7, 28, 35, and 42/43 from all controls and antibiotic-treated rats were measured and then transferred to labeled pre-cooled (dry-ice) DNA/RNA Shield-Lysis collection tubes (BIOZOL Diagnostica Vertrieb GmbH, Eching, Germany) avoiding excess thawing of the frozen fecal pellets. The processing of fecal slurries for further DNA isolation, quantification, sequencing, and the final report produced was based on the process previously published by Murali et al 2022 [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

“…This makes antibiotics ideal compounds to induce a temporary shift in the gut composition and to determine the changes in metabolic output. In previous studies, we have shown how different classes of antibiotics, including aminoglycosides, fluoroquinolones, lincosamides, and polymyxin E, induce selective changes in the gut microbiome of experimental animals and how these are reflected by antibiotic class-specific changes in the fecal and plasma metabolomes [ 1 , 11 , 12 , 13 ]. Several antibiotics belonging to classes including aminoglycosides, fluoroquinolones, lincosamides, and polymyxin E have been studied to understand their effects on the gut microbiota composition as well as the related metabolic outputs [ 1 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Gut Microbiota as Well as Metabolomes of Wistar Rats Recover within Two Weeks after Doripenem Antibiotic Treatment

et al. 2023

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An understanding of the changes in gut microbiome composition and its associated metabolic functions is important to assess the potential implications thereof on host health. Thus, to elucidate the connection between the gut microbiome and the fecal and plasma metabolomes, two poorly bioavailable carbapenem antibiotics (doripenem and meropenem), were administered in a 28-day oral study to male and female Wistar rats. Additionally, the recovery of the gut microbiome and metabolomes in doripenem-exposed rats were studied one and two weeks after antibiotic treatment (i.e., doripenem-recovery groups). The 16S bacterial community analysis revealed an altered microbial population in all antibiotic treatments and a recovery of bacterial diversity in the doripenem-recovery groups. A similar pattern was observed in the fecal metabolomes of treated animals. In the recovery group, particularly after one week, an over-compensation was observed in fecal metabolites, as they were significantly changed in the opposite direction compared to previously changed metabolites upon 28 days of antibiotic exposure. Key plasma metabolites known to be diagnostic of antibiotic-induced microbial shifts, including indole derivatives, hippuric acid, and bile acids were also affected by the two carbapenems. Moreover, a unique increase in the levels of indole-3-acetic acid in plasma following meropenem treatment was observed. As was observed for the fecal metabolome, an overcompensation of plasma metabolites was observed in the recovery group. The data from this study provides insights into the connectivity of the microbiome and fecal and plasma metabolomes and demonstrates restoration post-antibiotic treatment not only for the microbiome but also for the metabolomes. The importance of overcompensation reactions for health needs further studies.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%