Investigating the Glycating Effects of Glucose, Glyoxal and Methylglyoxal on Human Sperm

Nevin, Clare; McNeil, Lauren; Ahmed, Nessar; Murgatroyd, Chris; Brison, Daniel R.; Carroll, Michael A.

doi:10.1038/s41598-018-27108-7

Cited by 31 publications

(17 citation statements)

References 75 publications

(72 reference statements)

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“…In all cases, including the control, there was a time-dependent increase in crosslinking throughout the assay. These results are consistent with previous reports showing that MGO is a faster AGE generator than glucose (Nevin et al 2018). Overall, glucose seems to have a milder, more time-dependent effect, while MGO treatment appears to mimic the process of chemical crosslinking via external agents (Akhshabi et al 2018).…”

Section: Resultssupporting

confidence: 94%

Modulatory Effect of Glycated Collagen on Oral Streptococcal Nanoadhesion

et al. 2020

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Biofilm-mediated oral diseases such as dental caries and periodontal disease remain highly prevalent in populations worldwide. Biofilm formation initiates with the attachment of primary colonizers onto surfaces, and in the context of caries, the adhesion of oral streptococci to dentinal collagen is crucial for biofilm progression. It is known that dentinal collagen suffers from glucose-associated crosslinking as a function of aging or disease; however, the effect of collagen crosslinking on the early adhesion and subsequent biofilm formation of relevant oral streptococci remains unknown. Therefore, the aim of this work was to determine the impact of collagen glycation on the initial adhesion of primary colonizers such as Streptococcus mutans UA159 and Streptococcus sanguinis SK 36, as well as its effect on the early stages of streptococcal biofilm formation in vitro. Type I collagen matrices were crosslinked with either glucose or methylglyoxal. Atomic force microscopy nanocharacterization revealed morphologic and mechanical changes within the collagen matrix as a function of crosslinking, such as a significantly increased elastic modulus in crosslinked fibrils. Increased nanoadhesion forces were observed for S. mutans on crosslinked collagen surfaces as compared with the control, and retraction curves obtained for both streptococcal strains demonstrated nanoscale unbinding behavior consistent with bacterial adhesin-substrate coupling. Overall, glucose-crosslinked substrates specifically promoted the initial adhesion, biofilm formation, and insoluble extracellular polysaccharide production of S. mutans, while methylglyoxal treatment reduced biofilm formation for both strains. Changes in the adhesion behavior and biofilm formation of oral streptococci as a function of collagen glycation could help explain the biofilm dysbiosis seen in older people and patients with diabetes. Further studies are necessary to determine the influence of collagen crosslinking on the balance between acidogenic and nonacidogenic streptococci to aid in the development of novel preventive and therapeutic treatment against dental caries in these patients.

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Section: Resultssupporting

confidence: 94%

Modulatory Effect of Glycated Collagen on Oral Streptococcal Nanoadhesion

et al. 2020

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“…In this particular aspect, current extenders in use for stallion spermatozoa contain high concentrations of glucose, around 270–300 mM, these concentrations are far from being physiological, and may preclude long term preservation of liquid semen. It is well known that supraphysiological concentrations of glucose may lead to cell death [145] due to accumulation of advanced glycation end products (AGEs) [146,147,148,149]. The discovery of endocrine features in the spermatozoa also underlines the complex metabolism of these cells that represent an area of great interest for research in the coming decade [138,150].…”

Section: Redox Regulation and Sperm Metabolismmentioning

confidence: 99%

Redox Regulation and Oxidative Stress: The Particular Case of the Stallion Spermatozoa

et al. 2019

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Redox regulation and oxidative stress have become areas of major interest in spermatology. Alteration of redox homeostasis is recognized as a significant cause of male factor infertility and is behind the damage that spermatozoa experience after freezing and thawing or conservation in a liquid state. While for a long time, oxidative stress was just considered an overproduction of reactive oxygen species, nowadays it is considered as a consequence of redox deregulation. Many essential aspects of spermatozoa functionality are redox regulated, with reversible oxidation of thiols in cysteine residues of key proteins acting as an “on–off” switch controlling sperm function. However, if deregulation occurs, these residues may experience irreversible oxidation and oxidative stress, leading to malfunction and ultimately death of the spermatozoa. Stallion spermatozoa are “professional producers” of reactive oxygen species due to their intense mitochondrial activity, and thus sophisticated systems to control redox homeostasis are also characteristic of the spermatozoa in the horse. As a result, and combined with the fact that embryos can easily be collected in this species, horses are a good model for the study of redox biology in the spermatozoa and its impact on the embryo.

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“…Most studies use methylglyoxal or 3-DG to analyze the in vitro formation of AGEs. 24 25 But glyoxal is known to produce CML, 6 which is found in large quantities in the urine and serum of patients with diabetes. 3 26 27 As CML is a good biomarker of diabetes, glyoxal is one of the best glycating agents for in vitro studies of glycation.…”

Section: Discussionmentioning

confidence: 99%

Glycation by glyoxal leads to profound changes in the behavior of dermal fibroblasts

Guillon

Ferraro

Clément

et al. 2021

BMJ Open Diab Res Care

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IntroductionDiabetes is a worldwide health problem that is associated with severe complications. Advanced Glycation End products (AGEs) such as Nε-(carboxymethyl)lysine, which result from chronic hyperglycemia, accumulate in the skin of patients with diabetes. The effect of AGEs on fibroblast functionality and their impact on wound healing are still poorly understood.Research design and methodsTo investigate this, we treated cultured human fibroblasts with 0.6 mM glyoxal to induce acute glycation. The behavior of fibroblasts was analyzed by time-lapse monolayer wounding healing assay, seahorse technology and atomic force microscopy. Production of extracellular matrix was studied by transmission electronic microscopy and western blot. Lipid metabolism was investigated by staining of lipid droplets (LDs) with BODIPY 493/503.ResultsWe found that the proliferative and migratory capacities of the cells were greatly reduced by glycation, which could be explained by an increase in fibroblast tensile strength. Measurement of the cellular energy balance did not indicate that there was a change in the rate of oxygen consumption of the fibroblasts. Assessment of collagen I revealed that glyoxal did not influence type I collagen secretion although it did disrupt collagen I maturation and it prevented its deposition in the extracellular matrix. We noted a pronounced increase in the number of LDs after glyoxal treatment. AMPK phosphorylation was reduced by glyoxal treatment but it was not responsible for the accumulation of LDs.ConclusionGlyoxal promotes a change in fibroblast behavior in favor of lipogenic activity that could be involved in delaying wound healing.

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Investigating the Glycating Effects of Glucose, Glyoxal and Methylglyoxal on Human Sperm

Cited by 31 publications

References 75 publications

Modulatory Effect of Glycated Collagen on Oral Streptococcal Nanoadhesion

Modulatory Effect of Glycated Collagen on Oral Streptococcal Nanoadhesion

Redox Regulation and Oxidative Stress: The Particular Case of the Stallion Spermatozoa

Glycation by glyoxal leads to profound changes in the behavior of dermal fibroblasts

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