Investigating the Genetics of Populations

Hansen, Michael Møller; Villanueva, B.; Nielsen, Einar Eg; Bekkevold, Dorte

doi:10.1002/9780470995846.ch4

Cited by 5 publications

(3 citation statements)

References 95 publications

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“…Maintaining high genetic variation, as measured by high heterozygosity and allelic diversity, is crucial for keeping a population viable and able to adapt to environmental changes [1][2][3] . Conserving genetic diversity is important in conservation of captively propagated species.…”

Section: Genassemblage 20 Software Facilitates Conservation Of Genetmentioning

confidence: 99%

“…Conserving genetic diversity is important in conservation of captively propagated species. Unfortunately, genetic variation within captively propagated populations may decrease [4][5][6] due to (1) obtaining large groups of juveniles from a few parental individuals, (2) pairing individuals without knowledge of the genetic differences between them, (3) possible inbreeding events 7 , or (4) domestication of the broodstock 8 . A decrease in population genetic variation may reduce that population's viability and its potential to adapt to environmental stresses 3 .…”

Section: Genassemblage 20 Software Facilitates Conservation Of Genetmentioning

confidence: 99%

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Genassemblage 2.0 software facilitates conservation of genetic variation of captively propagated species

Kaczmarczyk

Wolnicki

2020

Sci Rep

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In conservation of captively propagated species, conserving genetic diversity is important. Here, we present an example of the use of Genassemblage 2.0 software in conserving the genetic variation of the lake minnow (Eupallasella percnurus). This fish has low genetic variation and is at risk of extinction in the western edge of its range, which includes Poland. Fish from one Polish population were captured (23 males, 25 females). Fin clips were taken, and DNA was extracted. Polymorphic microsatellites (13) were used to prepare genetic profiles, assess genetic variation in the fish and estimate genetic diversity in their progeny. Alleles were scored using an automatic capillary sequencer. The four and eight best variants of spawning pairs, and the optimal sets for group volitional breeding (four males, four females; eight males, eight females) were identified using Genassemblage 2.0. In the sets of 8 and 16 fish for group breeding, the mean heterozygosity, the number of alleles, and the share of “weak” heterozygotes (0.493, 24, 0.239 and 0.479, 23, 0.257, respectively) were better than the mean values for the progeny of all potential breeding pairs. For group volitional breeding, one set of four males and four females, and numerous sets of eight males and eight females would enable transmission of all 33 alleles identified in the potential broodstock and an expected progeny heterozygosity of 0.441 and 0.414, respectively. These expected heterozygosity values are higher than those in the broodstock. For practical purposes, the larger sets would be preferable for avoiding a future inbreeding and genetic drift.

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Section: Genassemblage 20 Software Facilitates Conservation Of Genetmentioning

confidence: 99%

Section: Genassemblage 20 Software Facilitates Conservation Of Genetmentioning

confidence: 99%

Genassemblage 2.0 software facilitates conservation of genetic variation of captively propagated species

Kaczmarczyk

Wolnicki

2020

Sci Rep

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“…Low values of (Ho), (He) and (A) indicate low genetic variation in the population (Szczecińska et al, 2016), and a statistically significant difference between (Ho) and (He) indicates a departure from Hardy-Weinberg equilibrium. This departure can reflect a disturbance in the genetic balance of the population, possibly connected with changes in population size or with non-random assemblage of individuals in reproductive pairs (Hansen et al, 2008). By comparing these indicators of genetic variation with historical data and data from other populations, the genetic diversity in a population can be assessed, and actions can be taken to counter diversity loss, if necessary (Kim et al, 2004).…”

Section: Detection and Monitoring Of Changes In Intrapopulation Genetic Variabilitymentioning

confidence: 99%

Techniques Based on the Polymorphism of Microsatellite Dna as Tools for Conservation of Endangered Populations

Kaczmarczyk¹

2019

Appl. Ecol. Env. Res.

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The reduction of the size and range of populations, and the destruction of their breeding sites has led to situations in which the existence of a population depends on breeding performed by humans in controlled conditions. Such conservation and restoration work should be based on maintaining the ability of protected populations to adapt to environmental conditions. This ability is determined by genetic variation within populations as well as individual genetic characteristics specific to a given population. Managing and preserving the genetic variation of a conserved population is one of most important factors that determine the success of a conservation program. This paper reviews a method based on DNA microsatellite polymorphism and its associated techniques for detection, management and conservation of genetic variation. It covers the use of this method in detecting and monitoring changes in intrapopulation genetic variability. It also covers the usefulness of this technique in assembling breeding pairs based on their genetic profiles, evaluating genetic differences between populations, optimizing and managing genetic variation resources deposited in banks of cryopreserved gametes, and evaluating the effectiveness of programs for conservation and restoration of species. Finally, suggestions for further development of the method are included.

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Adaptive strategies and life history characteristics in a warming climate: Salmon in the Arctic?

Nielsen¹,

Ruggerone²,

Zimmerman

2012

Environ Biol Fish

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Investigating the Genetics of Populations

Cited by 5 publications

References 95 publications

Genassemblage 2.0 software facilitates conservation of genetic variation of captively propagated species

Genassemblage 2.0 software facilitates conservation of genetic variation of captively propagated species

Techniques Based on the Polymorphism of Microsatellite Dna as Tools for Conservation of Endangered Populations

Adaptive strategies and life history characteristics in a warming climate: Salmon in the Arctic?

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