Investigating the feasibility of using DNA from sloughed skin for individual identification and kinship analysis in humpback whales (<i>Megaptera novaeangliae</i>)

Pierszalowski, Sophie; Ferrari, M. J.; Glockner-Ferrari, Debbie; Mizroch, Sally A.; Clapham, Phillip J.; Dickerson, Bobette

doi:10.1111/j.1748-7692.2012.00580.x

Cited by 5 publications

(5 citation statements)

References 25 publications

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“…Interestingly, combined approaches that monitor eDNA and/or eRNA of species of interest in combination with the pathogens, that affect it, were found to be key in implementing most appropriate mitigation strategies [51,56]. eDNA has also been shown to have utility as a population genetics tool [57] with mitochondrial DNA (mtDNA) and nuclear DNA (nuDNA) markers used to measure allelic diversity, kinship, sex ratios, abundance, population size patterns of dispersal and density [57][58][59][60][61]. Although the use of eDNA in population genetics is still in its beginning, a recent landmark study [62] showed its utility in examining shark population structure and their trophic interactions with the use mtDNA haplotype variations collected from seawater.…”

Section: Environmental Dna Monitoringmentioning

confidence: 99%

Emerging use of air eDNA and its application to forensic investigations – A review

Goray,

Taylor,

Bibbo

et al. 2024

Electrophoresis

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Biological material is routinely collected at crime scenes and from exhibits and is a key type of evidence during criminal investigations. Improvements in DNA technologies allow collection and profiling of trace samples, comprised of few cells, significantly expanding the types of exhibits targeted for DNA analysis to include touched surfaces. However, success rates from trace and touch DNA samples tend to be poorer compared to other biological materials such as blood. Simultaneously, there have been recent advances in the utility of environmental DNA collection (eDNA) in identification and tracking of different biological organisms and species from bacteria to naked mole rats in different environments, including, soil, ice, snow, air and aquatic. This paper examines the emerging methods and research into eDNA collection, with a special emphasis on the potential forensic applications of human DNA collection from air including challenges and further studies required to progress implementation.

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Section: Environmental Dna Monitoringmentioning

confidence: 99%

Emerging use of air eDNA and its application to forensic investigations – A review

Goray,

Taylor,

Bibbo

et al. 2024

Electrophoresis

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“…Additionally, it might be feasible to collect sloughed skin samples at the water surface after vigorous whale activities, such as mating, diving (e. g. sperm whales) or parturition. Such samples have been successfully used before ( Whitehead et al., 1990 , Valsecchi et al., 1998 , Pierszalowski et al., 2013 ), but their potential for parasitological analyses has not been clarified so far.…”

Section: Skin Biopsies and Blubber Samplesmentioning

confidence: 99%

Endo- and ectoparasites of large whales (Cetartiodactyla: Balaenopteridae, Physeteridae): Overcoming difficulties in obtaining appropriate samples by non- and minimally-invasive methods

Hermosilla

Silva

Prieto

et al. 2015

International Journal for Parasitology: Parasites and Wildlife

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Baleen and sperm whales, belonging to the Order Cetartiodactyla, are the largest and heaviest existent mammals in the world, collectively known as large whales. Large whales have been subjected to a variety of conservation means, which could be better monitored and managed if physiological and pathophysiological information, such as pathogen infections, could already be gathered from free-swimming animals instead of carcasses. Parasitic diseases are increasingly recognized for their profound influences on individual, population, and even ecosystem health. Furthermore, a number of parasite species have gained importance as opportunistic neozoan infections in the marine environment. Nonetheless, traditional approaches to study parasitic diseases have been impractical for large whales, since there is no current routine method for the capture and handling of these large animals and there is presently no practical method to obtain blood samples remotely from free-ranging whales. Therefore, we here not only intend to review the endo- and ectoparasite fauna of large whales but also to provide new insights in current available methods for gathering parasitological data by using non- or minimally invasive sampling techniques. We focus on methods, which will allow detailed parasitological studies to gain a broader knowledge on parasitoses affecting wild, free-swimming large whale populations.

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“…Microsatellites are an effective diversity analysis tool, they are widely distributed in the genomes of eukaryotes ( Brace et al, 1994 ; Pierszalowski et al, 2013 ) and have a high degree of conservation and generality, easy identification, wide distribution, high polymorphism, good reproducibility, can distinguish heterozygotes, and are very easy to mark and detect genetic material and traits between individuals and populations ( Liu et al, 2006 ; Muchadeyi et al, 2007 ; Wilkinson et al, 2012 ). Many studies have shown that they play an important role in analyzing the genetic diversity of poultry, and studying their origin, evolutionary direction, and existing crises and causes ( Koshiishi et al, 2020 ; Soglia et al, 2021 ; Zhang et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and breed identification of Chinese and Vietnamese local chicken breeds based on microsatellite analysis

Zhuang

Zhao

Zong

et al. 2023

Journal of Animal Science

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South Asia and Southeast Asia are the origins of domestic chickens and are rich in poultry genetic resources, resulting in many unique local chicken breeds. However, with the rapid intensification of poultry farming worldwide, many local chicken breeds are threatened with extinction. In response to China's "One Belt, One Road" policy, it is imperative to strengthen the conservation and breeding of local chicken breeds in China and Vietnam. This study characterized 18 microsatellite molecular genetic markers to analyze the genetic diversity of 21 local chicken populations in southern China (Yunnan and Guangxi Provinces) and Vietnam, Breed identification tags for microsatellite loci were constructed. The results showed that a total of 377 alleles were detected in all breeds, and the most alleles (44) and highest polymorphic information content (0.7820) were detected at the LEI0094 locus. The average polymorphic information content (PIC) content of the whole population was 0.65, indicating moderate polymorphism. The genetic diversity of the whole population was rich, except for two loci MCW0111 and MCW0016, that showed heterozygote excess at microsatellite loci, and the population had high genetic differentiation. The Vietnamese breeds showed low pairwise fixation coefficient (FST) and Nei's standard genetic distance (DS) between them. According to the neighbor-joining dendrogram constructed by DS and the analysis of population genetic structure using the Structure program, Longshengfeng chicken, Yunlong dwarf chicken, Tengchong white chicken, Xiayan chicken, and Daweishan mini chicken are similar, and Xishuangbanna game fowl, Wuding chicken, and Lanping silky chicken are similar to Yanjin black-bone chicken. In addition, excluding Dongtao chicken, other Vietnamese breeds are clustered together, indicating that the southern chicken breeds are closely related and have experienced better breeding. Overall, the whole population is rich in genetic resources, and the chicken breeds in the three regions are genetically close because of geographical factors and human activities. Dongtao chicken in Vietnamese, Chinese Yunnan local chicken breeds (Gallus gallus spadiceus), and red jungle fowl chickens (Gallus gallus) may have the same origin. We also constructed unique microsatellite molecular markers for 20 cultivars using 15 microsatellite loci. This study provides valuable insights to facilitate breed identification, and improve cultivar protection, and new germplasm construction.

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Investigating the feasibility of using DNA from sloughed skin for individual identification and kinship analysis in humpback whales (Megaptera novaeangliae)

Cited by 5 publications

References 25 publications

Emerging use of air eDNA and its application to forensic investigations – A review

Emerging use of air eDNA and its application to forensic investigations – A review

Endo- and ectoparasites of large whales (Cetartiodactyla: Balaenopteridae, Physeteridae): Overcoming difficulties in obtaining appropriate samples by non- and minimally-invasive methods

Genetic diversity and breed identification of Chinese and Vietnamese local chicken breeds based on microsatellite analysis

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