Investigating the Effects of Onion Juice on Male Fertility Factors and Pregnancy Rate After Testicular Torsion/Detorsion by Intrauterine Insemination Method

Abstract

have mentioned that infertility in 30% to 80% of men was related to the high level of ROS (4,6,7). Oxidative stress (OS) was found to damage the reproductive system and sperm, and therefore result in reducing sperm motility, lipid peroxidation, and oocyte-sperm fusion in addition to increasing the DNA damage (4). ROS, as stated by several studies, had considerable effects on spermatogenesis and sperm function. They reported that overproduction of ROS had harmful effects on motility, morphology, and concentration of sperm and that it caused sperm DNA damage and apoptosis (4,7-9). Testicular torsion, which occurs due to rotation of testicles, is a pathologic condition that leads to acute scrotal pain. According to the reports, although testicular torsion can be observed in any age group, its peak incidence usually occurs in young males aged 13-16 years. Moreover, it is estimated that 4.5 per 100 000 males within the age range of 1-25 years suffer from spermatic cord torsion (10). Furthermore, as pointed out by some studies, the degree and duration of Abstract Objectives: In this experimental study, a testicular torsion/detorsion model was used in male rats to investigate the effect of onion juice on male fertility factors and rate of pregnancy by intrauterine insemination (IUI). Materials and Methods: A total of 56 Wistar rats (28 males vs. 28 females) were included in this study. Male rats were randomly divided into 4 groups including sham, testicular torsion/detorsion, testicular torsion/detorsion treated with onion juice (40 mg/kg, orally) 30 minutes before detorsion, and those rats that were only treated with onion juice. Treatment with onion juice was continued for 14 days after detorsion. At the end of the study, following anesthesia with ketamine/xylazine (5/1 mg/kg), levels of testosterone hormone as well as some oxidative stress markers in the blood serum of the male rats were analyzed. Furthermore, sperm parameters were assayed, and then fertility power of the male rats was investigated in the adult female rats using IUI method. Results: The results of the study showed that there was a significantly negative change in TD group in terms of histological parameters while they were decreased in treated groups. On the one hand, although serum level of malondialdehyde (MDA) significantly increased in TD group, other oxidative markers' (i.e., glutathione peroxidase and superoxide dismutase) serum levels and also testosterone hormone significantly decreased, on the other as compared to the other groups. In addition, the rate of sperm parameters and pregnancy presence were lower in TD group than those of other groups. However, onion juice as a treatment factor could improve the sperm quality and fertility power. Conclusions: The obtained results revealed that using onion juice could enhance the quality of sperm and fertility power after testicular torsion/detorsion.

Protective effects ofStevia rebaudianaaqueous extract on experimental unilateral testicular ischaemia/reperfusion injury in rats

“…These changes of MDA, GPX and SOD levels in T/D group were also observed in similar studies that treatment with some antioxidant substances like verapamil, onion juice, Matricaria chamomilla, thymoquinone and quercetin could improve this biochemical condition to approximately healthy condition (Aktoz, Kanter, & Aktas, 2010;Ameli et al, 2018;Ayan et al, 2016;Shokoohi, Madarek, et al, 2018;Soltani et al, 2018). There are two major limitations in this study.…”

“…Superfluous amount of ROS is produced during this conversion. This excessive generation of ROS can even have harmful effects on sperm quality; these effects include reduction in normal morphology, normal motility, sperm count, and concentration of spermatozoa and even increase in abnormal spermatozoa (Shokoohi, Madarek, et al, 2018;. DNA damage, disorganisation in cell structure and function (due to cell membrane lipid peroxidation), and protein denaturation are outcomes of excessive generation of ROS (Moghimian et al, 2017;Wilhelm Filho et al, 2004).…”

“…DNA damage, disorganisation in cell structure and function (due to cell membrane lipid peroxidation), and protein denaturation are outcomes of excessive generation of ROS (Moghimian et al, 2017;Wilhelm Filho et al, 2004). This excessive generation of ROS can even have harmful effects on sperm quality; these effects include reduction in normal morphology, normal motility, sperm count, and concentration of spermatozoa and even increase in abnormal spermatozoa (Shokoohi, Madarek, et al, 2018;. In fact, oxidative stress is the main factor, which plays the key role in this injury (Ayan et al, 2016).…”

“…(Chatsudthipong & Muanprasat, 2009;Jayaraman et al, 2008;Shivanna et al, 2013;Shukla et al, 2013Shukla et al, , 2009Tadhani et al, 2007;Tadhani & Subhash, 2006;Takahashi et al, 2001). (2018), Shokoohi, Madarek, et al (2018) and Soltani et al (2018) in separated studies indicated that I/R injury is accompanied by destruction in tissue morphology and reduction of MTBS, and this situation can be reversed by use of a material with antioxidant features (Ameli et al, 2018;Shokoohi, Madarek, et al, 2018;Soltani et al, 2018).…”

Aflatoxin B1 induces oxidative stress in rats: possible protective effects of onion juice (Allium cepa L.)

“…Vitamin C was dissolved in water and administered in a dose of 10 mg/kg Nabzdyk and Bittner, (2018). Onion Juice (OJ) was administered in a dose of 40 ml/kg (Shokoohi et al, 2018). Both vitamin C and OJ were given by gastric intubation 2 hours before administration of AFB 1 .…”